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BLAST Search
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Query
Program
&
Database type
ORF DB
clone DB
genome DB
(contig DB)
protein
nucleotide
nucleotide
nucleotide
protein query
blastp
tblastn
tblastn
tblastn
nucleotide query
blastx
blastn
blastn
blastn
tblastx
tblastx
tblastx
Database
Eukaryota
A. oryzae
S. cerevisiae
Archaea
A. pernix
S. tokodaii
M. paludicola
P. horikoshii
Bacteria
K. setae
R. opacus
R. erythropolis
K. rhizophila
M. phosphovorus
C. efficiens
S. avermitilis
C. exile
A. thermophila
A. platensis
D. desulfuricans
S. haemolyticus
S. aureus N315
S. aureus MW2
B. brevis
O. valericigenes
G. aurantiaca
A. pasteurianus
A. multivorum
S. japonicum
Sphingobium sp.
D. magneticus
S. enterica
BLAST options
E-value
Filter
T
F
Additional parameters
Cost to open a gap
[Integer]
Cost to extend a gap
[Integer]
X dropoff value for gapped alignmentM
[Integer]
Show GI's in deflines
T
F
Penalty for a nucleotide mismatch (blastn only)
[Integer]
Reward for a nucleotide match (blastn only)
[Integer]
Number of one-line descriptions
[Integer: Max 100]
Number of alignments to show
[Integer: Max 50]
Threshold for extending hits
[Integer]
Perfom gapped alignment (not available with tblastx)
T
F
Query Genetic code to use
[Integer]
DB Genetic code (tblast[nx] only)
[Integer]
Believe the query defline
T
F
Matrix
BLOSUM45
BLOSUM62
BLOSUM80
PAM30
PAM70
MegaBlast search
T
F
Length of the largest intron allow in tblastn for linking HSPs
(0 disables linking)
[Integer]