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CDS Information : ANT_00050

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close this sectionGenomic map

Display Clone

Anaerolinea thermophila UNI-1T (= NBRC 100420T) chromosome

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Overview 
Genome
Proteome 
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close this sectionLocation

Organism Anaerolinea thermophila UNI-1T (= NBRC 100420T)
Replicon chromosome
Start / Stop / Direction 3,697 / 2,300 / -
Location complement(2300..3697)
Type CDS
Length 1,398 bp (465 aa)

close this sectionAnnotation

Product dihydrolipoamide dehydrogenase
Synonym: dihydrolipoyl dehydrogenase
Gene name lpd
Functional category Metabolism
- Carbohydrate Metabolism
EC number 1.8.1.4
Note The N-terminus of this ORF was identified by Edman sequencing.
KEGG pathway map:00010 KO:K00382
map:00020 KO:K00382
map:00252 KO:K00382
map:00260 KO:K00382
map:00620 KO:K00382
Sequence feature

41..46 (a.a.)DISULFID
444 (a.a.)ACT_SITE


References Family/Domain:IPR006258
Uniprot:P66004 Pubmed:11560483,16045627

close this sectionProteome

Proteome results
2D-PAGE (Protein Sequencer)
Show sequence

close this sectionComputational search results

BLASTP
Database:UniProtKB:2010_04
1E=1e-145D2B4F3_STRRDDihydrolipoyl dehydrogenaseAlignment
2E=1e-143D1AD37_THECDDihydrolipoyl dehydrogenaseAlignment
3E=1e-143C1YUH9_NOCDADihydrolipoyl dehydrogenaseAlignment
4E=1e-143D2PMS6_9ACTODihydrolipoamide dehydrogenaseAlignment
5E=1e-142C6WHK6_ACTMDDihydrolipoyl dehydrogenaseAlignment
HAMAP No significant hit
InterPro
Database:interpro:26.0
IPR000815 Mercuric reductase (Family)
 [15-33]  3.5e-12 PR00945 [50-69]  3.5e-12 PR00945 [140-157]  3.5e-12 PR00945 [175-192]  3.5e-12 PR00945 [195-210]  3.5e-12 PR00945 [422-441]  3.5e-12 PR00945
PR00945   HGRDTASE
IPR001100 Pyridine nucleotide-disulphide oxidoreductase, class I (Family)
 [5-27]  3.1e-69 PR00411 [37-52]  3.1e-69 PR00411 [139-148]  3.1e-69 PR00411 [175-200]  3.1e-69 PR00411 [263-277]  3.1e-69 PR00411 [306-313]  3.1e-69 PR00411 [342-363]  3.1e-69 PR00411 [407-422]  3.1e-69 PR00411 [429-449]  3.1e-69 PR00411
PR00411   PNDRDTASEI
IPR001327 Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region (Domain)
 [175-252]  9.6e-22 PF00070
PF00070   Pyr_redox
IPR004099 Pyridine nucleotide-disulphide oxidoreductase dimerisation region (Domain)
 [345-464]  3.7e-40 G3DSA:3.30.390.30
G3DSA:3.30.390.30   no description
 [346-455]  7.8e-36 PF02852
PF02852   Pyr_redox_dim
IPR006258 Dihydrolipoamide dehydrogenase (Family)
 [3-465]  1.5e-245 TIGR01350
TIGR01350   lipoamide_DH: dihydrolipoyl dehydrogenas
IPR012999 Pyridine nucleotide-disulphide oxidoreductase, class I, active site (Active_site)
 [38-48]  PS00076
PS00076   PYRIDINE_REDOX_1
IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase (Domain)
 [5-27]  1.7e-33 PR00368 [139-148]  1.7e-33 PR00368 [175-200]  1.7e-33 PR00368 [263-277]  1.7e-33 PR00368 [306-313]  1.7e-33 PR00368
PR00368   FADPNR
 [5-314]  1.9e-46 PF07992
PF07992   Pyr_redox_2
IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase (Family)
 [5-27]  1.7e-33 PR00368 [139-148]  1.7e-33 PR00368 [175-200]  1.7e-33 PR00368 [263-277]  1.7e-33 PR00368 [306-313]  1.7e-33 PR00368
PR00368   FADPNR
IPR016156 FAD/NAD-linked reductase, dimerisation (Domain)
 [343-464]  5.4e-41 SSF55424
SSF55424   FAD/NAD-linked reductases, dimerisation (C-terminal) domain
SignalP No significant hit
TMHMM No significant hit
SOSUI No significant hit

close this sectionCalculated information (Amino acid sequence)

size of protein 465 amino acids
molecular mass 49,791.32 Da
pI 6.2
aa composition
AlaValLeuIlePheTrpProMetGlySer
10.5
(49aa)
10.3
(48aa)
8.6
(40aa)
7.5
(35aa)
2.6
(12aa)
0.9
(4aa)
3.7
(17aa)
1.7
(8aa)
10.1
(47aa)
5.2
(24aa)
ThrCysAsnGlnTyrLysHisArgAspGlu
5.8
(27aa)
0.9
(4aa)
2.6
(12aa)
3
(14aa)
1.9
(9aa)
5.6
(26aa)
2.2
(10aa)
4.9
(23aa)
3.7
(17aa)
8.4
(39aa)

open this sectionSequence

close this sectionCovered clones

NBRC No. clone name start stop length(bp)
G21-001-001 AT1FE70L00014B05 712 39,427 38,716

close this sectionDistribution of Our Microbial Genomic DNA clones

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.