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CDS Information : ANT_06070

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close this sectionGenomic map

Display Clone

Anaerolinea thermophila UNI-1T (= NBRC 100420T) chromosome

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Overview 
Genome
Proteome 
Clone

close this sectionLocation

Organism Anaerolinea thermophila UNI-1T (= NBRC 100420T)
Replicon chromosome
Start / Stop / Direction 653,209 / 652,007 / -
Location complement(652007..653209)
Type CDS
Length 1,203 bp (400 aa)

close this sectionAnnotation

Product putative D-alanine--D-alanine ligase
Synonym: D-alanylalanine synthetase
Synonym: D-Ala-D-Ala ligase
Gene name
Functional category Environmental Information and Cellular Processing
- Cellular Processes
EC number 6.3.2.4
Note This ORF product lacks an active site (glutamic acid residue). This ORF product lacks an active site (serine residue).
KEGG pathway map:00473 KO:K01921
map:00550 KO:K01921
Sequence feature
References Uniprot:P07862 Pubmed:1554356,9054558
Uniprot:P0A6J8 Pubmed:1993184

close this sectionComputational search results

BLASTP
Database:UniProtKB:2010_04
1E=1e-56A9ETX0_SORC5D-alanine-D-alanine ligase / aminopeptidaseAlignment
2E=8e-55A6TSD6_ALKMQD-alanine--D-alanine ligaseAlignment
3E=2e-54A6GC53_9DELTD-alanine--D-alanine ligaseAlignment
4E=2e-54Q1D4Z8_MYXXDD-alanine-D-alanine ligase family proteinAlignment
5E=9e-53A9KRE2_CLOPHD-alanine--D-alanine ligase domain proteinAlignment
HAMAP No significant hit
InterPro
Database:interpro:26.0
IPR000291 D-alanine--D-alanine ligase/VANA/B/C, conserved site (Conserved_site)
 [309-337]  PS00844
PS00844   DALA_DALA_LIGASE_2
IPR000291 D-alanine--D-alanine ligase/VANA/B/C (Family)
 [309-337]  PS00844
PS00844   DALA_DALA_LIGASE_2
IPR011095 D-alanine--D-alanine ligase, C-terminal (Domain)
 [143-364]  1e-30 PF07478
PF07478   Dala_Dala_lig_C
IPR011761 ATP-grasp fold (Domain)
 [141-365]  36.599 PS50975
PS50975   ATP_GRASP
IPR013816 ATP-grasp fold, subdomain 2 (Domain)
 [192-368]  1.2e-25 G3DSA:3.30.470.20
G3DSA:3.30.470.20   no description
IPR016185 PreATP-grasp-like fold (Domain)
 [60-136]  1.4e-10 SSF52440
SSF52440   PreATP-grasp domain
SignalP No significant hit
TMHMM No significant hit
SOSUI No significant hit

close this sectionCalculated information (Amino acid sequence)

size of protein 400 amino acids
molecular mass 44,278.43 Da
pI 5.08
aa composition
AlaValLeuIlePheTrpProMetGlySer
10.3
(41aa)
5.5
(22aa)
10.3
(41aa)
6.8
(27aa)
3.8
(15aa)
1.5
(6aa)
8.5
(34aa)
1.5
(6aa)
7.3
(29aa)
4
(16aa)
ThrCysAsnGlnTyrLysHisArgAspGlu
5.5
(22aa)
0.8
(3aa)
2.8
(11aa)
3.3
(13aa)
2.5
(10aa)
2.8
(11aa)
3
(12aa)
6.8
(27aa)
5.8
(23aa)
7.8
(31aa)

open this sectionSequence

close this sectionCovered clones

NBRC No. clone name start stop length(bp)
G21-001-027 AT1FE70L00013A11 636,816 672,554 35,739

close this sectionDistribution of Our Microbial Genomic DNA clones

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.