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CDS Information : ANT_27350

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close this sectionGenomic map

Display Clone

Anaerolinea thermophila UNI-1T (= NBRC 100420T) chromosome

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Overview 
Genome
Proteome 
Clone

close this sectionLocation

Organism Anaerolinea thermophila UNI-1T (= NBRC 100420T)
Replicon chromosome
Start / Stop / Direction 3,017,156 / 3,016,701 / -
Location complement(3016701..3017156)
Type CDS
Length 456 bp (151 aa)

close this sectionAnnotation

Product methylglyoxal synthase
Gene name mgsA
Functional category Metabolism
- Carbohydrate Metabolism
EC number 4.2.3.3
Note The N-terminus of this ORF was identified by Edman sequencing.
KEGG pathway map:00620 KO:K01734
Sequence feature

71 (a.a.)ACT_SITE


References HAMAP:MF_00549

close this sectionProteome

Proteome results
2D-PAGE (Protein Sequencer)
Show sequence

close this sectionComputational search results

BLASTP
Database:UniProtKB:2010_04
1E=1e-52B8E059_DICTDMethylglyoxal synthaseAlignment
2E=2e-50B5YDH1_DICT6Methylglyoxal synthaseAlignment
3E=5e-48B0SS27_LEPBPMethylglyoxal synthase (MGS)Alignment
4E=5e-48B0S9D8_LEPBAMethylglyoxal synthaseAlignment
5E=8e-47D2C4K7_THENRMethylglyoxal synthaseAlignment
HAMAP No significant hit
InterPro
Database:interpro:26.0
IPR004363 Methylglyoxal synthase (Family)
 [12-131]  33.913 MF_00549
MF_00549   MGSA_MF_00549
 [65-73]  PS01335
PS01335   METHYLGLYOXAL_SYNTH
 [1-150]  3e-82 PIRSF006614
PIRSF006614   Methylglyoxal synthase
 [10-150]  8e-58 TIGR00160
TIGR00160   MGSA: methylglyoxal synthase
IPR011607 MGS-like (Domain)
 [1-148]  2.9e-57 SSF52335
SSF52335   Methylglyoxal synthase-like
 [40-117]  5.7e-14 PF02142
PF02142   MGS
 [1-150]  7.1e-55 G3DSA:3.40.50.1380
G3DSA:3.40.50.1380   no description
 [25-118]  1.7e-19 SM00851
SM00851   no description
IPR018148 Methylglyoxal synthase, active site (Active_site)
 [65-73]  PS01335
PS01335   METHYLGLYOXAL_SYNTH
SignalP No significant hit
TMHMM No significant hit
SOSUI No significant hit

close this sectionCalculated information (Amino acid sequence)

size of protein 151 amino acids
molecular mass 16,799.44 Da
pI 6.9
aa composition
AlaValLeuIlePheTrpProMetGlySer
7.9
(12aa)
6
(9aa)
12.6
(19aa)
7.3
(11aa)
4
(6aa)
1.3
(2aa)
6.6
(10aa)
2.6
(4aa)
6.6
(10aa)
6.6
(10aa)
ThrCysAsnGlnTyrLysHisArgAspGlu
4.6
(7aa)
0
(0aa)
2.6
(4aa)
2.6
(4aa)
2.6
(4aa)
6
(9aa)
4.6
(7aa)
4
(6aa)
6
(9aa)
5.3
(8aa)

open this sectionSequence

close this sectionCovered clones

NBRC No. clone name start stop length(bp)
G21-001-117 AT1FE70L00020D07 2,994,847 3,031,802 36,956

close this sectionDistribution of Our Microbial Genomic DNA clones

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.