CDS Information : AO090003000257

Display	1000bp 10000bp 100000bp Clone

Aspergillus oryzae RIB40 (= NBRC 100959) chromosome2	Legend Jump
Contig Overview  Genome Proteome  Clone

Center position	SC001SC003	change
ID		change
Gene name		change

Organism	Aspergillus oryzae RIB40 (= NBRC 100959)
Replicon	chromosome2
Contig	SC003
Start / Stop / Direction	682,684 / 684,869 / +
Location	join(682684..682865, 682925..683089, 683143..684248, 684304..684869)
Type	CDS
Length	2,019 bp (672 aa)
Intron	682866..682924, 683090..683142, 684249..684303

Product	molecular chaperones GRP78/BiP/KAR2, HSP70 superfamily
Gene name
Functional category	Cellular processes and signaling - [O] Posttranslational modification, protein turnover, chaperones
EC number
Note
KEGG pathway
Sequence feature
References

Proteome results	2D-PAGE YPD_liquid (1) 2D-PAGE YPD_filter (1) 1D-PAGE-LC YPD_liquid 1D-PAGE-LC YPD_filter MDLC YPD_liquide MDLC YPD_filter Show sequence

BLASTP Database:UniProtKB:2010_04 show BLAST table	1 E=0 Q9UWE3_ASPOR Molecular chaperones GRP78/BiP/KAR2 ER chaperone BiP Alignment 2 E=0 B8N4E9_ASPFN Hsp70 chaperone BiP/Kar2, putative Alignment 3 E=0 Q0CJU4_ASPTN 78 kDa glucose-regulated protein Alignment 4 E=0 A1DFN8_NEOFI ER Hsp70 chaperone BiP, putative Alignment 5 E=0 Q4WHP9_ASPFU Hsp70 chaperone BiP/Kar2, putative Alignment
HAMAP	No significant hit
InterPro	No significant hit
SignalP	Bacteria, Gram-positive    Eukaryota    Bacteria, Gram-negative
TMHMM	Transmembrane 1
SOSUI	Transmembrane 1

size of protein	672 amino acids
molecular mass	73,449.64 Da
pI	4.56
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 8.3 (56aa) 8.9 (60aa) 7.6 (51aa) 5.4 (36aa) 4.2 (28aa) 0.3 (2aa) 3.6 (24aa) 1.6 (11aa) 7.7 (52aa) 5.2 (35aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 7.4 (50aa) 0.1 (1aa) 5.1 (34aa) 3.3 (22aa) 2.4 (16aa) 8.3 (56aa) 0.9 (6aa) 3.4 (23aa) 8 (54aa) 8.2 (55aa)

>AO090003000257 molecular chaperones GRP78/BiP/KAR2, HSP70 superfamily
ATGGCGCGACTATCGAGTCGCAACGGTGCGGCCAAGCCGTTCACTGCTTGGACTACCATC
TTCTACCTTCTCCTTGTTTTCATCGCGCCCCTGGCATTCTTCGGTACCGCACACGCTGAG
GAGGACTCTGTCCAAGACAACTATGGAACTGTAATTGGTATTGATTTGGGAACAACCTAC
TCTTGTGTTGGTGTGATGCAGAATGGAAAGGTCGAGATTCTCGTCAACGACCAAGGAAAC
CGTATCACTCCTTCCTACGTCGCTTTCACCGATGAGGAACGCCTGGTCGGTGACGCCGCT
AAGAACCAATACGCCGCCAACCCCGTCAGGACCATCTTTGACATCAAGCGTCTGATTGGT
CGCAAGTACGATGACAAGGATGTCACCAAGGACACCAAGAACTTCCCCTTCAAGGTTGTC
AACAAGGATGGCAAGCCTGTCGTGAAGGTTGACGTGAACAAGACCCCCAAGACCTTCACT
CCTGAGGAGGTTTCCGCCATGGTCCTCGGAAAGATGAAGGAGATCGCTGAGGGCTACCTC
GGAAAGTCTGTCACCCACGCCGTCGTCACCGTCCCCGCCTACTTCAACGACGCCCAGAGA
CAGGCTACCAAGGACGCTGGTACCATCGCCGGTCTGAACGTTCTCCGTGTTGTCAACGAA
CCTACCGCCGCCGCTATCGCCTACGGATTGGACAAGACTGGTGATGAGCGCCAGGTCATC
GTCTACGATCTTGGTGGTGGTACTTTCGATGTCTCCCTTCTCTCCATTGACAACGGTGTC
TTCGAGGTTTTGGCTACCGCTGGTGACACTCACCTTGGTGGTGAGGACTTTGACCACCGT
GTCATGGACTACTTCGTCAAGCAGTACAACAAGAAGAACAACGTTGACATCACCAAGGAC
CTCAAGTCCATGGGTAAGCTCAAGCGCGAAGTCGAGAAGGCCAAGCGTACTCTCTCTTCC
CAGATGTCTACTCGCATTGAAATCGAGTCTTTCCACAACGGCGAGGACTTCTCTGAGACC
CTCACCCGTGCTAAGTTCGAAGAGCTGAACATGGATCTGTTCAAGAAGACCCTCAAGCCC
GTTGAGCAGGTGCTCAAGGACGCCAAGGTTAAGAAGTCCGAGGTTGACGACATTGTCCTT
GTTGGTGGATCTACCCGTATTCCCAAGGTCCAGGCTCTTCTCGAGGAGTTCTTCGGTGGC
AAGAAGGCCAGCAAGGGTATTAACCCTGATGAGGCTGTTGCTTTCGGTGCTGCCGTCCAG
GGTGGTGTCCTTTCCGGTGAGGCCGGTACCGAGGATGTCGTTCTGATGGACGTCAACCCT
CTTACCCTCGGTATCGAGACCACTGGCGGTGTCATGACTAAGCTCATCCCCCGTAACACC
GTTATCCCTACTCGCAAGTCCCAGATCTTCTCCACCGCCGCTGATAATCAGCCTACCGTT
CTTATCCAAGTTTATGAGGGCGAGCGTTCCTTGACCAAGGATAACAACCTCCTCGGCAAG
TTCGAGCTTACCAGCATTCCCCCCGCTCCCCGTGGTGTTCCCCAGATTGAGGTCTCCTTC
GATTTGGACGCCAACGGTATCCTGAAGGTCAGCGCCAGCGACAAGGGCACTGGCAAGGCT
GAGTCCATCACCATCACCAACGACAAGGGTCGTCTCTCCCAGGAGGAGATCGACCGCATG
GTCGCTGAGGCCGAAGAGTTCGCTGAGGAGGACAAGGCCATCAAGAGCAAGATCGAGGCC
CGCAACTCTCTGGAGAATTATGCCTTCAGCTTGAAGAACCAGGTCAACGATGAGAACGGC
TTGGGTGGCCAGATTGATGAGGACGACAAGCAGACCATCCTGGACGCCGTCAAGGAGGTC
ACTGACTGGCTCGAGGACAACGCTGCCGAAGCCACCACCGAGGACTTCGAGGAGCAGAAG
GAGCAGCTGTCCAACGTGGCCTACCCCATCACCAGCAAGCTCTATGGCTCTGCTCCGGCT
GATGAGGATGACGAGCCCTCTGGGCATGACGAACTGTAA


>AO090003000257 molecular chaperones GRP78/BiP/KAR2, HSP70 superfamily
MARLSSRNGAAKPFTAWTTIFYLLLVFIAPLAFFGTAHAEEDSVQDNYGTVIGIDLGTTY
SCVGVMQNGKVEILVNDQGNRITPSYVAFTDEERLVGDAAKNQYAANPVRTIFDIKRLIG
RKYDDKDVTKDTKNFPFKVVNKDGKPVVKVDVNKTPKTFTPEEVSAMVLGKMKEIAEGYL
GKSVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVLRVVNEPTAAAIAYGLDKTGDERQVI
VYDLGGGTFDVSLLSIDNGVFEVLATAGDTHLGGEDFDHRVMDYFVKQYNKKNNVDITKD
LKSMGKLKREVEKAKRTLSSQMSTRIEIESFHNGEDFSETLTRAKFEELNMDLFKKTLKP
VEQVLKDAKVKKSEVDDIVLVGGSTRIPKVQALLEEFFGGKKASKGINPDEAVAFGAAVQ
GGVLSGEAGTEDVVLMDVNPLTLGIETTGGVMTKLIPRNTVIPTRKSQIFSTAADNQPTV
LIQVYEGERSLTKDNNLLGKFELTSIPPAPRGVPQIEVSFDLDANGILKVSASDKGTGKA
ESITITNDKGRLSQEEIDRMVAEAEEFAEEDKAIKSKIEARNSLENYAFSLKNQVNDENG
LGGQIDEDDKQTILDAVKEVTDWLEDNAAEATTEDFEEQKEQLSNVAYPITSKLYGSAPA
DEDDEPSGHDEL


>AO090003000257 with intron
ATGGCGCGACTATCGAGTCGCAACGGTGCGGCCAAGCCGTTCACTGCTTGGACTACCATC
TTCTACCTTCTCCTTGTTTTCATCGCGCCCCTGGCATTCTTCGGTACCGCACACGCTGAG
GAGGACTCTGTCCAAGACAACTATGGAACTGTAATTGGTATTGATTTGGGAACAACCTAC
TCGTATGTTCTACAGCTGTGAGACAAGATAAAGGCGTTGCAATGACTAACTCCATCAACA
GTTGTGTTGGTGTGATGCAGAATGGAAAGGTCGAGATTCTCGTCAACGACCAAGGAAACC
GTATCACTCCTTCCTACGTCGCTTTCACCGATGAGGAACGCCTGGTCGGTGACGCCGCTA
AGAACCAATACGCCGCCAACCCCGTCAGGACCATCTTTGACATCAAGTGAGTTTCTCGGG
TTGGTTCGTATCATGAATCGGTGTGCTAATGTTTCCCAGGCGTCTGATTGGTCGCAAGTA
CGATGACAAGGATGTCACCAAGGACACCAAGAACTTCCCCTTCAAGGTTGTCAACAAGGA
TGGCAAGCCTGTCGTGAAGGTTGACGTGAACAAGACCCCCAAGACCTTCACTCCTGAGGA
GGTTTCCGCCATGGTCCTCGGAAAGATGAAGGAGATCGCTGAGGGCTACCTCGGAAAGTC
TGTCACCCACGCCGTCGTCACCGTCCCCGCCTACTTCAACGACGCCCAGAGACAGGCTAC
CAAGGACGCTGGTACCATCGCCGGTCTGAACGTTCTCCGTGTTGTCAACGAACCTACCGC
CGCCGCTATCGCCTACGGATTGGACAAGACTGGTGATGAGCGCCAGGTCATCGTCTACGA
TCTTGGTGGTGGTACTTTCGATGTCTCCCTTCTCTCCATTGACAACGGTGTCTTCGAGGT
TTTGGCTACCGCTGGTGACACTCACCTTGGTGGTGAGGACTTTGACCACCGTGTCATGGA
CTACTTCGTCAAGCAGTACAACAAGAAGAACAACGTTGACATCACCAAGGACCTCAAGTC
CATGGGTAAGCTCAAGCGCGAAGTCGAGAAGGCCAAGCGTACTCTCTCTTCCCAGATGTC
TACTCGCATTGAAATCGAGTCTTTCCACAACGGCGAGGACTTCTCTGAGACCCTCACCCG
TGCTAAGTTCGAAGAGCTGAACATGGATCTGTTCAAGAAGACCCTCAAGCCCGTTGAGCA
GGTGCTCAAGGACGCCAAGGTTAAGAAGTCCGAGGTTGACGACATTGTCCTTGTTGGTGG
ATCTACCCGTATTCCCAAGGTCCAGGCTCTTCTCGAGGAGTTCTTCGGTGGCAAGAAGGC
CAGCAAGGGTATTAACCCTGATGAGGCTGTTGCTTTCGGTGCTGCCGTCCAGGGTGGTGT
CCTTTCCGGTGAGGCCGGTACCGAGGATGTCGTTCTGATGGACGTCAACCCTCTTACCCT
CGGTATCGAGACCACTGGCGGTGTCATGACTAAGCTCATCCCCCGTAACACCGTTATCCC
TACTCGCAAGTCCCAGATCTTCTCCACCGCCGCTGATAATCAGCCTACCGTTCTTATCCA
AGTTTGTAAGTTTACCTTCTGGACGAAAGCCTTCTAGCAACCCACACTAACTGATCATAG
ATGAGGGCGAGCGTTCCTTGACCAAGGATAACAACCTCCTCGGCAAGTTCGAGCTTACCA
GCATTCCCCCCGCTCCCCGTGGTGTTCCCCAGATTGAGGTCTCCTTCGATTTGGACGCCA
ACGGTATCCTGAAGGTCAGCGCCAGCGACAAGGGCACTGGCAAGGCTGAGTCCATCACCA
TCACCAACGACAAGGGTCGTCTCTCCCAGGAGGAGATCGACCGCATGGTCGCTGAGGCCG
AAGAGTTCGCTGAGGAGGACAAGGCCATCAAGAGCAAGATCGAGGCCCGCAACTCTCTGG
AGAATTATGCCTTCAGCTTGAAGAACCAGGTCAACGATGAGAACGGCTTGGGTGGCCAGA
TTGATGAGGACGACAAGCAGACCATCCTGGACGCCGTCAAGGAGGTCACTGACTGGCTCG
AGGACAACGCTGCCGAAGCCACCACCGAGGACTTCGAGGAGCAGAAGGAGCAGCTGTCCA
ACGTGGCCTACCCCATCACCAGCAAGCTCTATGGCTCTGCTCCGGCTGATGAGGATGACG
AGCCCTCTGGGCATGACGAACTGTAA

NBRC No.	clone name	start	stop	length(bp)
G07-003-071	B068B01	504,330	703,713	199,384
G07-003-072	B048E09	506,579	692,844	186,266
G07-003-074	B060G12	529,485	722,148	192,664
G07-003-079	B033A04	576,263	696,172	119,910
G07-003-082	B033G10	597,573	716,562	118,990
G07-003-083	B030A08	613,236	778,508	165,273
G07-003-084	B041B12	626,941	694,464	67,524
G07-003-085	B018B10	629,723	701,354	71,632
G07-003-086	B039H03	629,727	694,903	65,177
G07-003-087	B087H12	641,837	830,562	188,726
G07-003-088	B047E02	641,838	740,140	98,303
G07-003-089	B053C06	649,882	774,350	124,469
G07-003-090	B037A04	671,686	846,176	174,491
G07-003-091	B076G02	673,853	806,550	132,698
G07-003-092	B030A07	675,446	774,360	98,915
G07-003-093	B011A12	680,134	805,669	125,536
G07-003-094	B052F01	680,171	768,790	88,620

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.