CDS Information : APA32_09550

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Acetobacter pasteurianus IFO 3283-32 (= NBRC 105190) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Acetobacter pasteurianus IFO 3283-32 (= NBRC 105190)
Replicon	chromosome
Start / Stop / Direction	988,494 / 989,513 / +
Location	988494..989513
Type	CDS
Length	1,020 bp (339 aa)

Product	DNA-directed RNA polymerase alpha subunit
Gene name	rpoA
Functional category	Information Pathways - Transcription
EC number
Note	Chromosome variant locus=SNP01 no-mutation Leu(CTG) at 249 a.a. was mutated to Gln(CAG) in the IFO 3283-26 and -03 strains.
KEGG pathway	map:00230 KO:K03040 map:00240 KO:K03040
Sequence feature	249 (a.a.) OTHER_PEP
References

BLASTP Database:UniProtKB:15.0 show BLAST table	1 E=1e-180 RPOA_GLUDA DNA-directed RNA polymerase subunit alpha Transcriptase subunit alpha RNA polymerase subunit alpha Alignment 2 E=1e-175 RPOA_GLUOX DNA-directed RNA polymerase subunit alpha Transcriptase subunit alpha RNA polymerase subunit alpha Alignment 3 E=1e-172 RPOA_GRABC DNA-directed RNA polymerase subunit alpha Transcriptase subunit alpha RNA polymerase subunit alpha Alignment 4 E=1e-158 RPOA_ACICJ DNA-directed RNA polymerase subunit alpha Transcriptase subunit alpha RNA polymerase subunit alpha Alignment 5 E=1e-154 A4U4G5_9PROT DNA-directed RNA polymerase alpha chain (RNAP alpha subunit) Alignment
HAMAP	No significant hit
InterPro Database:interpro:18.0	IPR009025 RNA polymerase, RBP11-like (Domain) SSF55257   RBP11-like subunits of RNA polymerase IPR011260 RNA polymerase, alpha subunit, C-terminal (Domain) SSF47789   C-terminal domain of RNA polymerase alpha subunit PF03118   RNA_pol_A_CTD PD001179   RPOA_AGRT5_Q9R710; IPR011261 RNA polymerase, dimerisation (Domain) PF01193   RNA_pol_L IPR011262 RNA polymerase, insert (Domain) PF01000   RNA_pol_A_bac SSF56553   Insert subdomain of RNA polymerase alpha subunit G3DSA:2.170.120.12   no description IPR011263 RNA polymerase, RpoA/D/Rpb3-type (Domain) SM00662   no description IPR011773 DNA-directed RNA polymerase, alpha subunit (Family) TIGR02027   rpoA: DNA-directed RNA polymerase, alpha su
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	339 amino acids
molecular mass	37,437.10 Da
pI	4.58
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 7.4 (25aa) 9.7 (33aa) 11.5 (39aa) 5.9 (20aa) 2.4 (8aa) 0.6 (2aa) 6.5 (22aa) 3.2 (11aa) 6.8 (23aa) 4.1 (14aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 4.4 (15aa) 0.6 (2aa) 5 (17aa) 4.1 (14aa) 1.5 (5aa) 4.7 (16aa) 0.9 (3aa) 5.9 (20aa) 5.9 (20aa) 8.8 (30aa)

>APA32_09550 DNA-directed RNA polymerase alpha subunit
TTGGTCCTTCAGAAAAACTGGCAATCTCTGATCAAGCCTGAAAAGCTTGAGGTCGAATCC
GGTGTGGAGCCAGCACGCATCGCAACCGTGGTTGCAGAACCTCTGGAACGCGGGTTCGGG
ATGACACTGGGGAATGCGCTGCGGCGTGTTCTGCTGTCCTCGCTTCAGGGTGCGGCTGTT
ACTGCTGTGCAGATTGACGGCGTGCTGCATGAGTTCTCGTCTGTTGCAGGCGTTCGGGAA
GATGTCACGGACATCGTGCTGAACATCAAGCAGCTGGCATTGCGTATGCATGGCGAAGGG
CCAAAGCGTATGATGCTGACGGCCACAGGCCCCGGCGAAGTGCGTGCCCGTCAGATCCAG
ACCGGCCATGATATCGAGATCATGAACCCCGATCTTGTTATCTGCACGCTGGACGATGGC
GTTAAGCTGGGCATGGAATTTATTGTAAATATGGGTAAAGGCTATGTGCCTGCCGCGGCT
AACCGCCCGGAAGATGCACCAATTGGCCTGATTCCGGTGGATGCAATTTATTCCCCCGTA
AAGCGCGTGTCTTATAAAGTTGAGCCTACCCGCGTTGGACAGGTGACCGACTATGATAAG
CTGCTGCTGACGGTTGAAACCAATGGTGCTGTAACACCTGAAGATGCTGTGGCTCTGGCT
GCACGTATTCTGCAGGATCAGCTCCAGCTGTTCATCAACTTTGATGAACCGCGCCCTGTG
CAGGCTGAAGAGCCTGAAGATGATCTGCCGTTCAACCGTAATCTGCTGCGTAAGGTGGAT
GAGCTTGAACTGTCTGTTCGGAGTGCAAACTGCCTGAAGAACGACAACATCATCTACATT
GGGGATCTGGTGCAGAAGAGCGAGCAGGAAATGCTCCGTACTCCGAACTTTGGCCGGAAA
TCCCTGAACGAAATCAAGGAAGTTCTCACCTCTATGGGGCTGTCCTTGGGTATGAATGTG
CCGGCTTGGCCGCCGGAAAATATTGAAGAGCTGGCAAAGCGGCTTGATGAGCCGTTTTGA


>APA32_09550 DNA-directed RNA polymerase alpha subunit
MVLQKNWQSLIKPEKLEVESGVEPARIATVVAEPLERGFGMTLGNALRRVLLSSLQGAAV
TAVQIDGVLHEFSSVAGVREDVTDIVLNIKQLALRMHGEGPKRMMLTATGPGEVRARQIQ
TGHDIEIMNPDLVICTLDDGVKLGMEFIVNMGKGYVPAAANRPEDAPIGLIPVDAIYSPV
KRVSYKVEPTRVGQVTDYDKLLLTVETNGAVTPEDAVALAARILQDQLQLFINFDEPRPV
QAEEPEDDLPFNRNLLRKVDELELSVRSANCLKNDNIIYIGDLVQKSEQEMLRTPNFGRK
SLNEIKEVLTSMGLSLGMNVPAWPPENIEELAKRLDEPF

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.