CDS Information : APA32_18746

Display	1000bp 10000bp 100000bp Clone

Acetobacter pasteurianus IFO 3283-32 (= NBRC 105190) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Acetobacter pasteurianus IFO 3283-32 (= NBRC 105190)
Replicon	chromosome
Start / Stop / Direction	2,034,834 / 2,038,922 / +
Location	2034834..2038922
Type	CDS
Length	4,089 bp (1,362 aa)

Product	DNA helicase restriction enzyme Type III R subunit
Gene name
Functional category	Information Pathways - Replication
EC number
Note	Probably pseudogene, N-terminal domain of a gene truncated by transposon Contains hyper-mutable tandem repeats AP-TR04. The repetitive number in the IFO 3283-22, IFO 3283-03, IFO 3283-12 are 30, 33, 35, respectively.
KEGG pathway
Sequence feature	1290..1357 (a.a.) REPEAT
References

BLASTP Database:UniProtKB:15.0 show BLAST table	1 E=0 Q4J601_AZOVI DEAD/DEAH box helicase:Helicase, C-terminal:Type III restriction enzyme, res subunit Alignment 2 E=0 Q1DBB9_MYXXD Putative restriction/modification enzyme Alignment 3 E=0 Q88BH4_PSESM Helicase domain protein Alignment 4 E=0 B0SIT9_LEPBA Helicase Alignment 5 E=0 B0SUG3_LEPBP Putative uncharacterized protein Alignment
HAMAP	No significant hit
InterPro Database:interpro:18.0	IPR001650 Helicase, C-terminal (Domain) PF00271   Helicase_C PS51194   HELICASE_CTER SM00490   no description IPR006935 Type III restriction enzyme, res subunit (Family) PF04851   ResIII IPR011545 DEAD/DEAH box helicase, N-terminal (Domain) SM00487   no description IPR014021 Helicase, superfamily 1 and 2, ATP-binding (Domain) PS51192   HELICASE_ATP_BIND_1
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	1362 amino acids
molecular mass	153,313.79 Da
pI	6.68
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 7.3 (99aa) 6.6 (90aa) 7.6 (104aa) 5.5 (75aa) 3.8 (52aa) 1.1 (15aa) 3.6 (49aa) 2.1 (28aa) 6.9 (94aa) 5.8 (79aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 6.7 (91aa) 1 (14aa) 4.3 (58aa) 5.7 (78aa) 2.9 (39aa) 6.5 (89aa) 2.6 (35aa) 6.2 (85aa) 7.4 (101aa) 6.4 (87aa)

>APA32_18746 DNA helicase restriction enzyme Type III R subunit
GTGGCGACCACAAACCACTGGAACAGTAACGCCGAAGACACACTAGCCAACCAGCACCCG
CCAGTTAGCAAGATTGACCTGCTCGACCTTGAAACCAGCATCATTGACTGGTCGCAATAT
CAGCCAAAACAGAAACCTGTCCTGCGGGAGAAAAAGAAGCCCCGCGAACATCAGGAAACA
GCCATCGGAAATGTGCTGGCGGGTTTCAAGACCCATAACCGTGGCCGTCTCATCATGGCT
TGTGGCACAGGTAAGACCTTCACAAGCCTGAAACTGGCAGAACAACTGGTTGGCCCTGGT
GGTCGCGTCCTGTTTCTGGTTCCCAGCCTGTCCCTACTCTCCCAATCGCTCACGGAATGG
ACCCAGGAAAGTCAGGTTCCCCTGCATAGCTTTGCCGTCTGTTCAGACAGTGATGTGGGA
AAGAAAAAGGCGTCCAACGATGACGAAATCAAGGTCAAGGTTCACGAGCTTCGCTACCCT
GCGACCACAAATCCTGAACGACTGGCGACCGAATACCAGAAACGTCATGATGCCACCCAT
ATGACGGTGGTTTTCTCCACCTATCATTCCATTGATGTCATCAGTCAGGCCCAGAAAGAA
TACGGCTTCCCGGAATTCGATCTAATTGTCTGCGATGAAGCTCACCGGACAACAGGCGTG
ACATTTGGTGGAGAAGAGAACGACAGCGCGTTTGTGAAAGTCCACAATCAGGATTATCTG
CACGGCAAACGTCGTCTCTACATGACAGCCACACCCCGTATCTATGGCGATGTGGCTCAG
GAGAAGGCCGAAAAGGAAGGAGCCATCGTCTATGGTATGAACAATGCCGAGATTTTTGGC
CCAGAGTTCCATGTCATCACCTTCTCTGAAGCGGTCAGACGCAAACTGCTGGTGGATTAT
AAGGTCATTGTGCTGGCTGTAGATGAAGGAACCGTCAGTGCAAGGCTTCAGAAGCTTCTG
GATGATCCAGACAATGGCCTGAAGGTCGATGATGCGTCCAAGATTGTCGGATGCTGGAAG
GCTCTCGCCAAAATCGGTCTGTCACAGGATGGTGTCGAAGACCCGGAACCTATGAAGCGG
GCTGTAGCGTTCTGCCAGGTTATCTCTCCCGATTACAAAGGACGCGCCCACAAGGTCAGT
TCCATCCAGATTGCCGAGATGTTCCAGAAAGTTGTTGAGGAATATCAGCAACAGGACGGT
ATCGAGCCAGAGGCACGGCTGACCTGCGAAGCGGAACACGTTGATGGCGGGATGAATGCC
AGTGAAAAGGAAGGCAAACTCTCCTGGCTGAAAGAGGAGACGCCAGACAAAACTTGCCGG
GTGCTGTCCAATGTCCGTTGTTTGTCTGAAGGTGTGGATGTTCCGGCTCTGGATGCCGTC
CTGTTCCTGACACCCCGCAACTCTCAGGTCGATGTGGTGCAGTCGGTCGGTCGCGTGATG
CGAAATGCTCCTGGCAAGAAACGGGGTTATGTGGTTCTGCCTGTTGTCATCCCTGCGGGA
ACACCTCCCGAACAGTCACTGGATAATAATCAGGCTTACAAGGTGGTCTGGCAGGTTCTT
CAGGCTCTGCGGTCTCACGATGACCGCTTCGACAACATGGTCAACAAAATGGACCTTCAG
GCGAAACCTGACACGTCCCGCATGGAAGTCGTCGCCATCACCCAGAAGATTACCCAGAAG
ACGATGGCTCTGACGACTGGAACGGCTCAGAAAGCTCATTCGTCATACTCCCTTGGCAAG
AAAGCCTCTCGTCCCAGCGAAGATCAATTCAAAATGGAGTTCGAAGTCGGGGAAATCGAA
CGTGCTATCTATGCGAAAATCGTCAAGAAAGTCGGCAATCGCCACCATTGGGAGGACTGG
GCTGGGGATATTGCGAAAATTGCCCAGACCCACATTACCCGTATCACCACCATTCTTGAG
AACCCGGAGAATGTTGGTGCCCGTAAAGCCTTTGACCAGTTTGCAGGAGACCTGCGTTCA
GAACTGAATGACAGCATCACGGATGAGGAAATCATCGAGATGCTGGCACAGCATCTGATT
ACCCGTCCGGTCTTCGAAGCCCTATTCTCAGGCCACAGTTTTGTTTCCGATAATCCCATG
TCGAAAGCCATGCAGTCCGTTCTGGATGCGTTGGACAAACACAGCCTGCACAAGGAAGCA
GACACCTTGGAAGCGTTCTATGCCAATGTGCGGGAACGTGCTTCCGGAATTGATACGGCC
TATGGTCGTCAGAAGGTCATCAAGGAACTGTATGACGGTTTTTTCCAGACGGCGTTTCCA
AAACTCAAGGAACGGCTTGGCATCGTCTATACCCCTCTTGAAGTGGTAGACTTCATCATC
CGCTCCATCAACGATGTCTTGGAAAATGAATTTGGCCAGACACTTGGAAGCAAAGGCGTC
CACATCATGGACCCCTTTACCGGGACCGGAACCTTCATCACCCGGCTGTTGCAAAGTGGT
CTGATTACGAAGGAACAACTTCGTCACAAATACAAACAGGAACTGCATGCCAATGAAATC
GTCCTGCTAGCTTACTACATCGCTTCCATCAACATCGAAGCCACATTCTCCGACCTGATG
GACGGAGCCTATGAGCCGTTTGAGGGCATCTGTTTAACCGACACGTTCCGCCTCAGTGAA
CCACATGATCTGATCGGGTCCACGCTGGAAGATAACAATAAGCGTATCCGAAAGCAGAAA
AAGCTCGATATACGTGTCATCATGGGGAACCCGCCCTATTCGGTAGGACAGGAAAGCGGA
AACGACAACAATCAGAACGTATCCTATCCTGCCCTTGATGAACGTATTGCCAAAACCTAT
GCGGAACGTTCAACGGCAACGAACAAACGTGCTCTGTACGACAGCTACATCCGGGCAATC
CGCTGGGCTTCTGATCGTATTGGAAACTGTGGCGTTCTCGGATTTGTGACCAATGCGGGC
TTTCTGGATGCCAATACAGCCAATGGTCTGCGTCAGTGTCTGGCAGAAGAGTTCTCCTCC
ATCCATGTGTTCCATCTGCGGGGAAACCAGCGGACTTCAGGTGAAACGTCCCGCAAGGAA
GGAGGCAAGATTTTTGATGCAGGAAGTCGCGCTCCGATTGCCATTTCCATCATGGTCAAG
AACCCGGATGCAAAGGAACAGGGACGGATCCTGTTCCATGACATCGGAGATTATCTGACC
CGTGAGCAGAAGCTGAAACATATTGAAGCACTGGCCAGTGTCAACGGTCTGACGGATGCA
GGGCTGTGGCAGAACATCACGCCTGATGAGCACGGAGACTGGCTCAGACAGAGAGATGAC
AACTTCGGGCAGTTCATTGCGATTGGAAGCAAGGACAAGGATGCTGGGACAGTTCTATTT
TCTACATATTCAGCGGGAGTCAAAACCCAGCGGGACGCATGGTGCTGGAATGCTGGGAAA
AAGGCTCTGGCAAACAACATGCAAACCACCATTTCGGCCTATAATCAGGAACTGATGCGA
TTTAATGAAGCATTCCCGGTCTTGCACAGGCAGACACGAGACAAAGAGGTCGATAAGTTT
ATTGATACCAACCCAACAAAAATTAGTTGGACCCGCGCTCTAAAGCAGGATTTGGTCAAA
GGAAAAGACCTTCTTTATTCCTCTAATTGTCTTGTGAAGGGTGTTTACAGACCATTCACG
TTCCAATGGCTCTATTTCAACAGGCGCATGAACGAAATGGTCTACCAGATGCCTCGGCTG
TTTCCTGATGCGAAGGCAGATAATAGAATAATTATGATCAAGCAACGCCAGCCTGATGAC
AGTCAATTCGTACTGATGAGTAAGCACGTCCCAGACCTGCAAAGCGATGGGGGAACGCAA
TGCTTTTCTGAATATCTATACGAAGAGGACAGGACAGGACAGGACAGGACAGGACAGGAC
AGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGAC
AGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGAC
AGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGACAGGACAATTGTACTC
GCTTACTAA


>APA32_18746 DNA helicase restriction enzyme Type III R subunit
MATTNHWNSNAEDTLANQHPPVSKIDLLDLETSIIDWSQYQPKQKPVLREKKKPREHQET
AIGNVLAGFKTHNRGRLIMACGTGKTFTSLKLAEQLVGPGGRVLFLVPSLSLLSQSLTEW
TQESQVPLHSFAVCSDSDVGKKKASNDDEIKVKVHELRYPATTNPERLATEYQKRHDATH
MTVVFSTYHSIDVISQAQKEYGFPEFDLIVCDEAHRTTGVTFGGEENDSAFVKVHNQDYL
HGKRRLYMTATPRIYGDVAQEKAEKEGAIVYGMNNAEIFGPEFHVITFSEAVRRKLLVDY
KVIVLAVDEGTVSARLQKLLDDPDNGLKVDDASKIVGCWKALAKIGLSQDGVEDPEPMKR
AVAFCQVISPDYKGRAHKVSSIQIAEMFQKVVEEYQQQDGIEPEARLTCEAEHVDGGMNA
SEKEGKLSWLKEETPDKTCRVLSNVRCLSEGVDVPALDAVLFLTPRNSQVDVVQSVGRVM
RNAPGKKRGYVVLPVVIPAGTPPEQSLDNNQAYKVVWQVLQALRSHDDRFDNMVNKMDLQ
AKPDTSRMEVVAITQKITQKTMALTTGTAQKAHSSYSLGKKASRPSEDQFKMEFEVGEIE
RAIYAKIVKKVGNRHHWEDWAGDIAKIAQTHITRITTILENPENVGARKAFDQFAGDLRS
ELNDSITDEEIIEMLAQHLITRPVFEALFSGHSFVSDNPMSKAMQSVLDALDKHSLHKEA
DTLEAFYANVRERASGIDTAYGRQKVIKELYDGFFQTAFPKLKERLGIVYTPLEVVDFII
RSINDVLENEFGQTLGSKGVHIMDPFTGTGTFITRLLQSGLITKEQLRHKYKQELHANEI
VLLAYYIASINIEATFSDLMDGAYEPFEGICLTDTFRLSEPHDLIGSTLEDNNKRIRKQK
KLDIRVIMGNPPYSVGQESGNDNNQNVSYPALDERIAKTYAERSTATNKRALYDSYIRAI
RWASDRIGNCGVLGFVTNAGFLDANTANGLRQCLAEEFSSIHVFHLRGNQRTSGETSRKE
GGKIFDAGSRAPIAISIMVKNPDAKEQGRILFHDIGDYLTREQKLKHIEALASVNGLTDA
GLWQNITPDEHGDWLRQRDDNFGQFIAIGSKDKDAGTVLFSTYSAGVKTQRDAWCWNAGK
KALANNMQTTISAYNQELMRFNEAFPVLHRQTRDKEVDKFIDTNPTKISWTRALKQDLVK
GKDLLYSSNCLVKGVYRPFTFQWLYFNRRMNEMVYQMPRLFPDAKADNRIIMIKQRQPDD
SQFVLMSKHVPDLQSDGGTQCFSEYLYEEDRTGQDRTGQDRTGQDRTGQDRTGQDRTGQD
RTGQDRTGQDRTGQDRTGQDRTGQDRTGQDRTGQDRTIVLAY

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.