CDS Information : APA32_19020

Display	1000bp 10000bp 100000bp Clone

Acetobacter pasteurianus IFO 3283-32 (= NBRC 105190) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Acetobacter pasteurianus IFO 3283-32 (= NBRC 105190)
Replicon	chromosome
Start / Stop / Direction	2,068,824 / 2,069,762 / +
Location	2068824..2069762
Type	CDS
Length	939 bp (312 aa)

Product	D-alanine--D-alanine ligase
Gene name
Functional category	Metabolic
EC number
Note
KEGG pathway	map:00473 KO:K01921 map:00550 KO:K01921
Sequence feature
References

BLASTP Database:UniProtKB:15.0 show BLAST table	1 E=1e-115 A9H0J6_GLUDA UDP-N-acetylenolpyruvoylglucosamine reductase Alignment 2 E=1e-115 B5ZJU5_GLUDA D-alanine/D-alanine ligase Alignment 3 E=1e-107 DDL_GLUOX D-alanine--D-alanine ligase D-alanylalanine synthetase D-Ala-D-Ala ligase Alignment 4 E=1e-106 DDL_GRABC D-alanine--D-alanine ligase D-alanylalanine synthetase D-Ala-D-Ala ligase Alignment 5 E=1e-101 DDL_ACICJ D-alanine--D-alanine ligase D-alanylalanine synthetase D-Ala-D-Ala ligase Alignment
HAMAP	No significant hit
InterPro Database:interpro:18.0	IPR000291 D-alanine--D-alanine ligase/VANA/B/C, conserved site (Conserved_site) PS00844   DALA_DALA_LIGASE_2 PS00843   DALA_DALA_LIGASE_1 IPR000291 D-alanine--D-alanine ligase/VANA/B/C (Family) PS00843   DALA_DALA_LIGASE_1 PS00844   DALA_DALA_LIGASE_2 IPR005905 D-alanine--D-alanine ligase (Family) TIGR01205   D_ala_D_alaTIGR: D-alanine--D-alanine ligas PTHR23132   D-ALANINE--D-ALANINE LIGASE IPR011095 D-alanine--D-alanine ligase, C-terminal (Domain) PF07478   Dala_Dala_lig_C IPR011127 D-alanine--D-alanine ligase, N-terminal (Domain) PF01820   Dala_Dala_lig_N IPR011761 ATP-grasp fold (Domain) PS50975   ATP_GRASP IPR013816 ATP-grasp fold, subdomain 2 (Domain) G3DSA:3.30.470.20   no description IPR013817 Pre-ATP-grasp fold (Domain) G3DSA:3.40.50.20   no description IPR016185 PreATP-grasp-like fold (Domain) SSF52440   PreATP-grasp domain
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	312 amino acids
molecular mass	32,944.36 Da
pI	4.84
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 12.5 (39aa) 8.7 (27aa) 8.7 (27aa) 4.8 (15aa) 1.9 (6aa) 1 (3aa) 5.4 (17aa) 2.6 (8aa) 9 (28aa) 6.4 (20aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 7.4 (23aa) 1.9 (6aa) 2.6 (8aa) 4.5 (14aa) 2.9 (9aa) 2.9 (9aa) 2.2 (7aa) 4.2 (13aa) 6.4 (20aa) 4.2 (13aa)

>APA32_19020 D-alanine--D-alanine ligase
ATGAATGCACCAAAACGCGTAACCGTGCTGATGGGTGGCATGTCCTCCGAACGGGTGGTC
AGCCTTTCATCCGGTCAAGGCGTGGCGGAAGCCCTGCGCAGCAAAGGGTATGATGTCTGC
ACGCTGGATGCTCAAGCTGATCTGGCTACGTTAGTTGAAGCGTTAAACACCCAAAAACCG
GATGTGGTGTTTAACGCCCTGCATGGTCGTTTTGGTGAGGATGGCTGTATTCAGGGTATA
CTGGACTGGATGGGTATTCCCTACACCCATTCGGGCATTCGGGCCTCTGCTACTGCTATG
GATAAAGCCGCTGCCCGCGCTATTTTCCAAACAGCCGGGCTGCCCGTGGCAGAAGGCATG
GTGGTAGATATTGCCACCCTTGCTCATGCAGACCCCCTGCCTACACCTTACGTGGTGAAG
CCGCTTAATGAAGGCTCATCCGTTGGGGTTTCCATTGTGCGTACGGGATCCAACACGCGC
GCTGATATTGTTAAAAACTGGACTTACGGCCCGCAAGCGCTTGTAGAAGCCTTTATTCCC
GGTCGTGAAATTACCGTAGGTGTTATGGGTGATCGGGCGCTCACCATTACGGATATTACC
CCTACCGGCACCGGGCATGATTTTTACGACTATGATTCCAAATATCAGGTCGGCGGCTCT
GCCCATGTTCTGCCTGCACAAATTGATGCCGCCAGCGCCAAACTTGCTCTGGAAGTTGCC
GTAAAAGCGCATCAGGCATTGGGGTGTTCTGGCGCATCGCGCTCTGATTTCCGGTTGGAT
GATACCAACCCGGATCAGCCACACCTTATTCTGCTGGAGGTCAACACGCAGCCGGGTATG
ACGGCCACATCCCTGCTGCCCGAACAGGCCGCCTATTGTGGTATCAGCTACCCCGAACTT
TGCGCGTGGTTGGTTGAACAGGCTACCTGCCGGCAATGA


>APA32_19020 D-alanine--D-alanine ligase
MNAPKRVTVLMGGMSSERVVSLSSGQGVAEALRSKGYDVCTLDAQADLATLVEALNTQKP
DVVFNALHGRFGEDGCIQGILDWMGIPYTHSGIRASATAMDKAAARAIFQTAGLPVAEGM
VVDIATLAHADPLPTPYVVKPLNEGSSVGVSIVRTGSNTRADIVKNWTYGPQALVEAFIP
GREITVGVMGDRALTITDITPTGTGHDFYDYDSKYQVGGSAHVLPAQIDAASAKLALEVA
VKAHQALGCSGASRSDFRLDDTNPDQPHLILLEVNTQPGMTATSLLPEQAAYCGISYPEL
CAWLVEQATCRQ

NBRC No.	clone name	start	stop	length(bp)
G15-001-101	AS1FE12L00021F05	2,040,596	2,075,526	34,931
G15-001-102	AS1FE12L00002H08	2,062,267	2,099,891	37,625

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.