CDS Information : BBR47_00270

Display	1000bp 10000bp 100000bp Clone

Brevibacillus brevis NBRC 100599 chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Brevibacillus brevis NBRC 100599
Replicon	chromosome
Start / Stop / Direction	42,657 / 43,661 / +
Location	42657..43661
Type	CDS
Length	1,005 bp (334 aa)

Product	putative histidinol-phosphatase
Gene name	hisK
Functional category	Intermediary metabolism - Metabolism of amino acids and related molecules
EC number	3.1.3.15
Note	This ORF product does not have significant similarity to HisK in Bacillus subtilis.
KEGG pathway	map:00340 KO:K01089 ec:3.1.3.15
Sequence feature
References	Uniprot:O34411 Pubmed:10322033

BLASTP Database:UniProtKB:13.0 show BLAST table	1 E=1e-117 Q4MSP0_BACCE Histidinol-phosphatase Alignment 2 E=1e-117 Q73B87_BACC1 Histidinol phosphatase, putative Alignment 3 E=1e-117 Q81T57_BACAN Histidinol phosphatase, putative Alignment 4 E=1e-117 B0AQK6_BACAN Putative histidinol phosphatase Alignment 5 E=1e-117 A0RBM5_BACAH Histidinol-phosphatase Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR004013 PHP, C-terminal (Domain) PF02811   PHP IPR010140 Histidinol phosphate phosphatase, HisJ (Family) TIGR01856   hisJ_fam: histidinol phosphate phosphatase PTHR21039   HISTIDINOL PHOSPHATASE-RELATED IPR016195 Polymerase/histidinol phosphatase-like (Domain) SSF89550   PHP domain-like
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	334 amino acids
molecular mass	38,752.91 Da
pI	4.79
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 7.2 (24aa) 5.4 (18aa) 13.2 (44aa) 4.2 (14aa) 5.4 (18aa) 2.1 (7aa) 4.2 (14aa) 2.4 (8aa) 5.7 (19aa) 5.7 (19aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 4.8 (16aa) 1.2 (4aa) 3 (10aa) 2.4 (8aa) 5.4 (18aa) 1.8 (6aa) 3.9 (13aa) 7.2 (24aa) 6.6 (22aa) 8.4 (28aa)

>BBR47_00270 putative histidinol-phosphatase
ATGTTGATCGACTATCATCTTCATCTAGAAGAAGGGCCTTTTTCATTGCGTTGGCTCGAC
CGTACCAATATGGCTCTCGACCATTTTTACCCGTTGACTGAACCTCGACATACCCGTGCC
TGGCTGCTGGACAGTCTTGCGCGATTAAATAACCGCATGTCTTTGGGGGCATACGACCCA
TCCTGGATCGATCTGTATTTACGTGAAGCGTTAAACAAAGGCTTGAAGGAAGTGGGGATC
GTCGATCATTTATACCGCTTTCGTGAAGCCCGTCCTTACTTTGAACGATATATGGAGCTG
GGGGACACCGAGTTGGGTCGTTTGCAGCGAACGTGGCTTAATCAAGTATGCACTGAGAGC
CTCAGTGACTTTTGTGTCGCCATTGAGGAAGCAAAGCAGCGTTGGTCGGCAAGCGGTGTG
GAGTTGCGGTTGGGGCTGGAAGCGGATTACTTTATTGGCGGGGAAGCAGAATTGGAAAGC
TTGCTCGCAGGTGCTTCGTGGGATTATGTGATCGGTTCTGTTCATTTTTTGCAAGGATGG
GGCTTTGACAATCCCGAGACTCGCCATCTGTTTGAGCAGCATGACCTGAAACAGCTATAC
ACGGACTTTTTCCACACCGTTGAGTCCATGATCAGGAGCAACTTGTTTGATTTTGTTGCC
CATCTGGACAATTTGAAAGTGTTCTCCTATCGTCCAGAGGAATCGGAGCTTGTTCCCTAT
TACCACCGAATCGCAACTGCTTTGAAGGAAACGGATACAGCGACCGAAATCAATGCGGGC
TTGTACTACCGCTATCCCGTTCAGGAAATGTGCCCCAGTCCCGCTTTCCTCGATGTTTTG
GTGGCGCATGGTGTTCCACTGACACTCTCGTCTGACGCGCACTTTCCTGATGATATTGGC
AGATATGTGGCGGTGAATCTGGAGATCCTCGATTCAATGGGGGTAACGGAGATTGCCACT
TTTTTCGGTCGTCAACGGATCATGAGGCCAATCTGCTATGCTTGA


>BBR47_00270 putative histidinol-phosphatase
MLIDYHLHLEEGPFSLRWLDRTNMALDHFYPLTEPRHTRAWLLDSLARLNNRMSLGAYDP
SWIDLYLREALNKGLKEVGIVDHLYRFREARPYFERYMELGDTELGRLQRTWLNQVCTES
LSDFCVAIEEAKQRWSASGVELRLGLEADYFIGGEAELESLLAGASWDYVIGSVHFLQGW
GFDNPETRHLFEQHDLKQLYTDFFHTVESMIRSNLFDFVAHLDNLKVFSYRPEESELVPY
YHRIATALKETDTATEINAGLYYRYPVQEMCPSPAFLDVLVAHGVPLTLSSDAHFPDDIG
RYVAVNLEILDSMGVTEIATFFGRQRIMRPICYA

NBRC No.	clone name	start	stop	length(bp)
G10-001-002	BB1FE1L10023G09	26,882	59,887	33,006
G10-001-003	BB1FE1L10009G06	40,439	77,360	36,922

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.