CDS Information : BBR47_02930

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Brevibacillus brevis NBRC 100599 chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Brevibacillus brevis NBRC 100599
Replicon	chromosome
Start / Stop / Direction	292,923 / 294,449 / +
Location	292923..294449
Type	CDS
Length	1,527 bp (508 aa)

Product	putative chitinase D precursor
Gene name	chiD
Functional category	Other functions - Miscellaneous
EC number	3.2.1.14
Note	chiD in Bacillus circulans.
KEGG pathway
Sequence feature
References	Uniprot:P27050 Pubmed:8168626,1729234

BLASTP Database:UniProtKB:13.0 show BLAST table	1 E=1e-164 CHID_BACCI Chitinase D precursor Alignment 2 E=1e-159 Q099U8_STIAU Chitinase D Alignment 3 E=1e-156 Q1CZN0_MYXXD Chitinase, class II Alignment 4 E=1e-125 Q092X1_STIAU Chitinase D Alignment 5 E=1e-117 Q81A65_BACCR Exochitinase Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR001223 Glycoside hydrolase, family 18, catalytic domain (Domain) PF00704   Glyco_hydro_18 IPR001223 Glycoside hydrolase, family 18 (Family) PF00704   Glyco_hydro_18 IPR001579 Glycoside hydrolase, chitinase active site (Active_site) PS01095   CHITINASE_18 IPR003610 Carbohydrate-binding family V/XII (Domain) SM00495   no description G3DSA:2.10.10.20   no description SSF51055   Carbohydrate binding domain PF02839   CBM_5_12 IPR003961 Fibronectin, type III (Domain) PS50853   FN3 PF00041   fn3 SM00060   no description IPR008957 Fibronectin, type III-like fold (Domain) G3DSA:2.60.40.30   no description SSF49265   Fibronectin type III IPR011583 Chitinase II (Domain) SM00636   no description IPR011583 Chitinase II (Family) SM00636   no description IPR013781 Glycoside hydrolase, catalytic core (Domain) G3DSA:3.20.20.80   no description IPR017853 Glycoside hydrolase, catalytic core (Domain) SSF51445   (Trans)glycosidases
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit
SOSUI	Transmembrane 1

size of protein	508 amino acids
molecular mass	54,819.78 Da
pI	5.99
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 8.7 (44aa) 6.7 (34aa) 8.3 (42aa) 4.9 (25aa) 4.1 (21aa) 1.6 (8aa) 6.1 (31aa) 2.8 (14aa) 8.3 (42aa) 9.6 (49aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 9.3 (47aa) 0.2 (1aa) 5.3 (27aa) 3.9 (20aa) 4.5 (23aa) 3.7 (19aa) 2 (10aa) 2.4 (12aa) 4.7 (24aa) 3 (15aa)

>BBR47_02930 putative chitinase D precursor
TTGATGTATTTTTTGATGAGAAAAGCAGGAGTTTTGGTAGTACTATCGCTTCTCTTAATT
TTTTCGCTATTCCCGCATTCTTCTCACGCCATGACACCGTGGACTCCCAACACCGTATAC
AAGGTAGGAGACATGGTTACGTATCAAGAGCAAGATTACAAATGTACATTTGCTCATACA
TCGCTTGTCGGGTGGGAACCGCCTAACGTACCGACACTGTGGCAGCCGCAAGGTCCCTCT
GCTCCCGATACCATTCCACCTACGTCTCCCACAAATCTTAGTGAAGTAGGGAAAACGAGT
AGTTCAGTTAGCTTGTCTTGGTCAGCTTCTACGGATGATAGAGCTGTTAAGGAGTATCTC
GTCTACAACGGAGATACACTCGCTGGTATATCGAGTACACCATCCTATACCGTGACAGGG
CTTCTAGCGAATACGACATATTCATTCACGATCCAGGCAAAAGATGCCGCAGGTAATGTA
TCCCCACCCAGCGCCTCGATAACGGTAACCACTTTATCTACATCCCCGAATGAACCGGTG
TCCAAACGAGTTTTGATTGGCTACTGGCATAATTTTGACAATGGTTCAACAGTACTCAAG
CTAAGAGATGTCTCAGATAAATACGATGTAATCAATGTTGCGTTCGCTGAACCAGTCGGT
GGCGATCATGCGACAATGGGATTTGTGCCGTTCAATGCATCCGTTGAGGAATTTAAGTCG
GATATCGCCTTGTTACAGTCAAAGGGGAAGAAGGTCCTCATCTCGATTGGCGGAGCGAAT
GGTACAGTCGAGCTGACGACGGAAGCAGCCAAACAAAGTTTTATTACCTCGATGACCTCG
ATTATTCAAACGTACGGGTTTGATGGTATGGATATTGATCTGGAAGGAAGCTCCTTGTCG
CTAAATGCGGGGGACACTGATTTTAGAAATCCGACAACGCCCAAAATTATAAACTTGATC
GCAGCGACTCAGACGATCACGAATACATTTGGCCCATCTTTCATTTTGACAATGGCGCCA
GAAACGGCTTATGTCCAAGGAGGTTACGCTGCCTATGGTGGGCCGTGGGGCGCTTATCTG
CCTGTGATTCATGCCCTTCGCGACCGGATGAATTATATCCACGTTCAGCACTATAATTCT
GGAGCGCTAGAAGCATTGGATGGCCGTACGTATCAACAAGGAACGGCTGATTTTCAAGTA
GCGATGGCAGAAATGCTATTGAAAGGATTCCCTATCGGGCGGAATCCAAACAACATGTTT
CCAGCCTTGAGGGAAAACCAAGTCGCGATCGGGCTGCCTTCTGCTTCAAGTGCTGCCGGG
GGAGGCTATACGTCTCCTGCCGATATCCAGAAAGCCCTTCACTATTTGGTAAAGGGTACG
TCTTTTGGGGGAACCTACAAACTCCAAAATCTAGCTGGTTATCCTCAGTTCCAAGGAGTT
ATGACCTGGTCTATCAACTGGGATGCTAGAAATAACTATTCGTTTGCCAATCATGTTCGC
ACTTCGCTTGATTCGTTGAGCCAGTAA


>BBR47_02930 putative chitinase D precursor
MMYFLMRKAGVLVVLSLLLIFSLFPHSSHAMTPWTPNTVYKVGDMVTYQEQDYKCTFAHT
SLVGWEPPNVPTLWQPQGPSAPDTIPPTSPTNLSEVGKTSSSVSLSWSASTDDRAVKEYL
VYNGDTLAGISSTPSYTVTGLLANTTYSFTIQAKDAAGNVSPPSASITVTTLSTSPNEPV
SKRVLIGYWHNFDNGSTVLKLRDVSDKYDVINVAFAEPVGGDHATMGFVPFNASVEEFKS
DIALLQSKGKKVLISIGGANGTVELTTEAAKQSFITSMTSIIQTYGFDGMDIDLEGSSLS
LNAGDTDFRNPTTPKIINLIAATQTITNTFGPSFILTMAPETAYVQGGYAAYGGPWGAYL
PVIHALRDRMNYIHVQHYNSGALEALDGRTYQQGTADFQVAMAEMLLKGFPIGRNPNNMF
PALRENQVAIGLPSASSAAGGGYTSPADIQKALHYLVKGTSFGGTYKLQNLAGYPQFQGV
MTWSINWDARNNYSFANHVRTSLDSLSQ

NBRC No.	clone name	start	stop	length(bp)
G10-001-009	BB1FE1L10029E04	265,703	304,114	38,412
G10-001-010	BB1FE1L10033D08	292,603	334,912	42,310

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.