CDS Information : BBR47_18030

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Brevibacillus brevis NBRC 100599 chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Brevibacillus brevis NBRC 100599
Replicon	chromosome
Start / Stop / Direction	1,895,921 / 1,896,850 / +
Location	1895921..1896850
Type	CDS
Length	930 bp (309 aa)

Product	porphobilinogen deaminase
Gene name	hemC
Functional category	Intermediary metabolism - Metabolism of coenzymes and prosthetic groups
EC number	2.5.1.61
Note	hemC in Bacillus subtilis.
KEGG pathway
Sequence feature
References	HAMAP:MF_00260 Uniprot:P16616 Pubmed:1672867 Uniprot:O69104 Pubmed:10217486 Uniprot:O69110 Pubmed:10217486

BLASTP Database:UniProtKB:13.0 show BLAST table	1 E=1e-98 Q2B6L0_9BACI Porphobilinogen deaminase Alignment 2 E=3e-98 HEM3_BACHD Porphobilinogen deaminase PBG Hydroxymethylbilane synthase HMBS Pre-uroporphyrinogen synthase Alignment 3 E=1e-97 HEM3_BACA2 Porphobilinogen deaminase PBG Hydroxymethylbilane synthase HMBS Pre-uroporphyrinogen synthase Alignment 4 E=5e-97 B0AUZ3_BACAN Porphobilinogen deaminase Alignment 5 E=5e-97 A7GTE5_BACCN Porphobilinogen deaminase Alignment
HAMAP Database:HAMAP:20080521	MF_00260   HEM3
InterPro Database:interpro:16.0	IPR000860 Porphobilinogen deaminase (Family) SSF54782   Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain PR00151   PORPHBDMNASE PD002745   HEM3_PAEMA_O69110; PF01379   Porphobil_deam TIGR00212   hemC: porphobilinogen deaminase PS00533   PORPHOBILINOGEN_DEAM G3DSA:3.30.160.40   no description PF03900   Porphobil_deamC PTHR11557   PORPHOBILINOGEN DEAMINASE IPR022417 Porphobilinogen deaminase, N-terminal (Domain) PF01379   Porphobil_deam IPR022418 Porphobilinogen deaminase, C-terminal domain (Domain) PF03900   Porphobil_deamC G3DSA:3.30.160.40   no description SSF54782   Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain IPR022419 Porphobilinogen deaminase, dipyrromethane cofactor binding site (Binding_site) PS00533   PORPHOBILINOGEN_DEAM
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	309 amino acids
molecular mass	33,387.15 Da
pI	4.69
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 9.7 (30aa) 7.1 (22aa) 12.3 (38aa) 5.5 (17aa) 3.6 (11aa) 0.6 (2aa) 4.2 (13aa) 1.6 (5aa) 9.4 (29aa) 4.9 (15aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 5.2 (16aa) 0.6 (2aa) 2.6 (8aa) 4.9 (15aa) 0.6 (2aa) 6.1 (19aa) 1 (3aa) 5.2 (16aa) 7.1 (22aa) 7.8 (24aa)

>BBR47_18030 porphobilinogen deaminase
ATGGGAAAATGGAAAGTGGGAACGCGTCGCAGCAAATTGGCGCTCACTCAAACGAATTGG
GTCGTTGATAAACTGAAGGGGTTCGCACCTGAGGCTGATTTTGAATTGCATGAAATCGTG
ACGAAAGGCGATCGAATCCTCGATGTGACCTTGTCCAAAGTTGGCGGAAAAGGATTGTTT
GTCAAAGAAATCGAGCAATCGTTGTTCGATAAGGAAACCGATTTTGCGGTACACAGCTTG
AAGGATATGCCTGCTGAATTGCCAGATGGCTTGGTAATCGGAGCGATTCCGAAGCGTGTT
GATCCTCGAGATGTCCTGCTTTCCAAGGACGGCAAGACGTTGGATGAATTACCACAAGGC
GCTTTGGTAGGGACCAGTAGCTTGCGCCGTTCTTCACAGATTCTCGCATATCGCCCTGAT
ATTCAGATCGAATCCTTGCGTGGAAATATTGATACGCGCATGCGTAAGCTTGCGGAAGGA
AATTTTGATGCGATCATCCTTGCTGCGGCTGGACTTGAGCGCGTGAATTTTGAAGGGGAA
ATCTCGCAGTTCCTGCCTGTTGAAATCAGCTTGCCAGCAGTCGGTCAAGGTGCGCTTGCC
ATCGAATGCCGAGCAGACGATGAGGAGACACTCGCTCTCTTGAAGCAGTTTGATGACGCA
CCGACGCGTTTGGCGGTATCGGCAGAGCGGAGCTTCCTGCACAAGCTCCAAGGGGGCTGT
CAGGTGCCGATTGGCGCATATGCGACAGTTGGAGAGAACAATGAAATTACGTTGACCGGA
ATGGTTGGCTCCCCAGATGGGAAGCAAATGTTCAAAAACACGGCGACTGGCCAGGATCCG
TTAGCGCTGGGTATTCAAGTAGCAGAAGCACTTCTGGCGCAAGGAGCGGGAGACGTACTT
GCGGAAGTATTGCGGGAGAATGAGCAATGA


>BBR47_18030 porphobilinogen deaminase
MGKWKVGTRRSKLALTQTNWVVDKLKGFAPEADFELHEIVTKGDRILDVTLSKVGGKGLF
VKEIEQSLFDKETDFAVHSLKDMPAELPDGLVIGAIPKRVDPRDVLLSKDGKTLDELPQG
ALVGTSSLRRSSQILAYRPDIQIESLRGNIDTRMRKLAEGNFDAIILAAAGLERVNFEGE
ISQFLPVEISLPAVGQGALAIECRADDEETLALLKQFDDAPTRLAVSAERSFLHKLQGGC
QVPIGAYATVGENNEITLTGMVGSPDGKQMFKNTATGQDPLALGIQVAEALLAQGAGDVL
AEVLRENEQ

NBRC No.	clone name	start	stop	length(bp)
G10-001-068	BB1FE1L10034H01	1,863,299	1,901,579	38,281
G10-001-069	BB1FE1L10028B03	1,891,723	1,927,120	35,398

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.