CDS Information : BBR47_18040

Display	1000bp 10000bp 100000bp Clone

Brevibacillus brevis NBRC 100599 chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Brevibacillus brevis NBRC 100599
Replicon	chromosome
Start / Stop / Direction	1,896,847 / 1,897,659 / +
Location	1896847..1897659
Type	CDS
Length	813 bp (270 aa)

Product	uroporphyrinogen-III synthase
Gene name	hemD
Functional category	Intermediary metabolism - Metabolism of coenzymes and prosthetic groups
EC number	4.2.1.75
Note	hemD in Bacillus subtilis.
KEGG pathway
Sequence feature
References	Uniprot:P21248 Pubmed:1672867

BLASTP Database:UniProtKB:13.0 show BLAST table	1 E=6e-56 A0LN72_SYNFM Uroporphyrinogen-III synthase / uroporphyrinogen-III C-methyltransferase Alignment 2 E=1e-53 A5D3L6_PELTS Uroporphyrinogen-III methylase and Uroporphyrinogen-III synthase Alignment 3 E=1e-52 Q1AUK4_RUBXD Uroporphyrinogen-III C-methyltransferase / uroporphyrinogen-III synthase Alignment 4 E=2e-52 A4J6H6_DESRM Uroporphyrinogen-III C-methyltransferase / uroporphyrinogen-III synthase Alignment 5 E=6e-51 A1HSI5_9FIRM Uroporphyrin-III C-methyltransferase Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR003754 Uroporphyrinogen III synthase HEM4 (Domain) SSF69618   Uroporphyrinogen III synthase (U3S, HemD) PF02602   HEM4
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	270 amino acids
molecular mass	29,279.90 Da
pI	6.18
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 11.9 (32aa) 8.9 (24aa) 11.5 (31aa) 6.3 (17aa) 3 (8aa) 0.7 (2aa) 4.8 (13aa) 2.6 (7aa) 7.4 (20aa) 4.4 (12aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 6.3 (17aa) 0.7 (2aa) 1.9 (5aa) 3.3 (9aa) 2.2 (6aa) 4.4 (12aa) 3.7 (10aa) 4.4 (12aa) 4.1 (11aa) 7.4 (20aa)

>BBR47_18040 uroporphyrinogen-III synthase
ATGATTGTCCACGGATCGCACAAGCCGCTGACTGGCAAGTGCATCATGGTCACCCGGGCA
AGAAGTCAAGTTCGCGAGCTCGTTGAACAGATCGAGCGGTTGGGTGGAGAAGCATACGCA
TTTCCCCTGCTAAAAATGATGCCGCCGACGGACACTGCCAAGCTCGACGAAGCCATCACC
CAGTTGCCAGCATTCGACTGGGTGGTCTTCACGAGTGTGAATGGCGTGCGCTTTTTCTTG
GAGCGCATGCGCGAGGTCGGGGTTGGCCTTGAAGCGTTCACTGGCAAAATTGCTGCGGTC
GGTCCGAAAACGGCCAAGGCGCTTGAGCATCACGGGCTCGAAGTAGCTGTCATCCCGTCT
GATTACGTCGCGGAGGGCTTGCTGTCGAGCTTGTATGATCAGTTGCTTCCTGGTCAGCGA
GTTCTGCTGCCTCGTGCAGATATTGCGCGCAAGGCATTGCCAAAGGAGTTGGCTCGCTTG
GGGCTTGCTGTGACAGAGGTAGACGTGTATCACACTGTCATTGATGCAGAGCACGCTCCA
GAAGCAGCGGAAAAACTACAGCAAGGTCTCATTGATATCATCTTGTTTACCAGCTCTTCA
ACGGTGACGCATTTCATGGCAGCGATGGAGCCCTATGCATCGTTAGACTGGCTCAAGCAC
GTTCAGATAGCCTGTATCGGACCGATAACGGCAGATACAGCAAAGCAAAACGGACTTTCT
GTTCATGCAGTAGCAAGTGAGTACACCGTGGAAGGATTACTAGCAGCAATCATAGAGAAT
CTGGGAGGGAATCGACATGGCACAAACATTTGA


>BBR47_18040 uroporphyrinogen-III synthase
MIVHGSHKPLTGKCIMVTRARSQVRELVEQIERLGGEAYAFPLLKMMPPTDTAKLDEAIT
QLPAFDWVVFTSVNGVRFFLERMREVGVGLEAFTGKIAAVGPKTAKALEHHGLEVAVIPS
DYVAEGLLSSLYDQLLPGQRVLLPRADIARKALPKELARLGLAVTEVDVYHTVIDAEHAP
EAAEKLQQGLIDIILFTSSSTVTHFMAAMEPYASLDWLKHVQIACIGPITADTAKQNGLS
VHAVASEYTVEGLLAAIIENLGGNRHGTNI

NBRC No.	clone name	start	stop	length(bp)
G10-001-068	BB1FE1L10034H01	1,863,299	1,901,579	38,281
G10-001-069	BB1FE1L10028B03	1,891,723	1,927,120	35,398

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.