CDS Information : BBR47_22040

Display	1000bp 10000bp 100000bp Clone

Brevibacillus brevis NBRC 100599 chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Brevibacillus brevis NBRC 100599
Replicon	chromosome
Start / Stop / Direction	2,301,583 / 2,303,448 / +
Location	2301583..2303448
Type	CDS
Length	1,866 bp (621 aa)

Product	putative 5,10-methylenetetrahydrofolate reductase
Gene name
Functional category	Intermediary metabolism - Metabolism of amino acids and related molecules
EC number	1.5.1.20
Note
KEGG pathway
Sequence feature
References	Uniprot:O06745 Pubmed:10094622,15215334,18205390

BLASTP Database:UniProtKB:13.0 show BLAST table	1 E=1e-172 Q9KCE2_BACHD Methylenetetrahydrofolate reductase Alignment 2 E=1e-170 Q5L228_GEOKA Methylenetetrahydrofolate reductase Alignment 3 E=1e-170 A2UAD0_BACCO Methylenetetrahydrofolate reductase Alignment 4 E=1e-169 A3I969_9BACI Bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein Alignment 5 E=1e-168 A4IL00_GEOTN Methylenetetrahydrofolate reductase Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR003171 Methylenetetrahydrofolate reductase (Family) PF02219   MTHFR IPR003726 Homocysteine S-methyltransferase (Domain) PF02574   S-methyl_trans G3DSA:3.20.20.330   no description PS50970   HCY SSF82282   Homocysteine S-methyltransferase
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	621 amino acids
molecular mass	67,918.75 Da
pI	6.05
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 11.3 (70aa) 7.4 (46aa) 11.1 (69aa) 6 (37aa) 3.2 (20aa) 0 (0aa) 4 (25aa) 2.3 (14aa) 7.4 (46aa) 5.2 (32aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 5.3 (33aa) 1.1 (7aa) 3.5 (22aa) 3.9 (24aa) 3.1 (19aa) 3.9 (24aa) 2.3 (14aa) 6.8 (42aa) 5.6 (35aa) 6.8 (42aa)

>BBR47_22040 putative 5,10-methylenetetrahydrofolate reductase
TTGACGACGAGAAAGAAAGATTTACGAGCCTACTTGAAAGACCATCTCCTAGTAGGAGAT
GGTGCAATGGCGACCCAGCTGCACGGGCTGGGCGTCCCGGTTGGCGTCAGCTTTGAGGAG
CTGTGTTTGTCCAACCCTCGTTTGGTGCATGAAGTTCATACGTCTTATTACCAGGCCGGT
GCGCGTTTCCTAGAGACAAATACGTATTCTGCCAACCGAGATGCATTGAGCCGTTACGGT
CTGGAGCATAAGGTAGCCAGGATTAACCGGCTCGCAGTCGCCATTGCACGGGAAGCCGCA
CAGGACGACGCTTATGTGGCTGGAAGCATCGGATCGATTTTGGCAGGACGGGTGAAGAAA
AAGGTTTTGGATGAATACCGTGACCAGTATGAGGAACAGGCCATTGCCCTGTTACATGCG
GGGGTAGACGGACTGATTCTGGAGACGTTCTTGGACTTGGAGGAGCTGCTTCTGGCGATC
GAGGTAATCCGCCCGCTCACAGATGTCCCTGTAATCGCGCAGTTAGCCACTTTGGAAGTA
GGGCGCACACGTGATGGTTACGCTTTGACAGATGCCTTTTCCGAGCTGAAAGCAGCGGGC
GCTGACGTGGTCGGACTAAACTGTCGACTTGGTCCCGCTGAACTCTTGCGCTCGTTTGAA
AATACAGTGATTCCAGAAGATGCACTCCTATCCGTATTCCCGAATGCGGGGCGTCTGGGG
ATGACTGACGGAGAATACGCTTTTAAATCATCACCTGAATACTTTGGACAAAGTGCGCTT
CGCTTGCGTGAGCAGGGAGTTCGGTTGATTGGTGGATGCTGCGGAACCACACCTGCACAT
GTGAAGGCGATTGCGGATGCACTTGAATCTCTTGAACCGCAAGCGCGTGTGAACCCGACC
ATCGTTGCTGGAGATCGTCCTACGATTTCTGTGCAGAACACGAGGGAGAGGGAGAAACCA
AGCATTGTCGAGCGTGTAAAAACAGCAACGACAATCATTGTCGAGTTTGATCCGCCACGT
GATCTGGATGCAGACCAATTTCTCCACGGATGCTGTGAGCTGCACAAAGCAGGAGCAGAC
GCGATTACGTTGGCGGATAACTCGTTGGCGACAGTGCGCATGAGCAACATGGCTCTCGGT
GCCCTCATGAAGAGCAGACATGGAATCGATCCGCTCTTGCACCTTGCTTGCCGGGATCGC
AATCTGATTGGACAGCAATCGCACTTAATGGGCCTCAACGCTTTGGGGATTGACCAAATA
TTGGTGATTACTGGTGATCCATCACGCTTTGGTGACTTGCCAGGGGCCAGCTCGGTATTT
GATGTGACCTCGTTTGATCTGATTCGCATGGTGAAGCAGTTGAACGAAGGTGTTTCGTTC
TCAGGACGTCCGTTAAAGCAAAAAGCCCAGTTTATCGTGGGCGCTGCCTTTAATCCGAAT
GTCCGAAATATGGATGCTGCCGTTGCCCGCTTGGAAAAGAAGGTCGAGGCAGGCGCGGAT
TACATTATGACGCAGCCTGTGTACGATGCGGAGTCGATTCGGAATGTATACGAGGCGACG
AAGCATATTGGCATTCCTGTCTTTATCGGGATCATGCCGCTGACCAGCTCGCGCAATGCG
GAGTTCCTGCATAATGAAGTGCCGGGAATCAAGCTATCGACAGAAGCTCTAGAACGGATG
AAAAAGGTACAGGGAGAAGCCGCCAGACAGGAAGGAATCGCAATCGCGAAGGAATTGGTT
GATACGGCCGTTCGTTACTTCAATGGCATCTATTTGATTACACCTTTTAATTATTACGAG
ATGGCGGCAGAGCTCATTCAATATACCCGCCAACAAAGCTCTTTGGTGAGGATGGCTCAG
ACGTAA


>BBR47_22040 putative 5,10-methylenetetrahydrofolate reductase
MTTRKKDLRAYLKDHLLVGDGAMATQLHGLGVPVGVSFEELCLSNPRLVHEVHTSYYQAG
ARFLETNTYSANRDALSRYGLEHKVARINRLAVAIAREAAQDDAYVAGSIGSILAGRVKK
KVLDEYRDQYEEQAIALLHAGVDGLILETFLDLEELLLAIEVIRPLTDVPVIAQLATLEV
GRTRDGYALTDAFSELKAAGADVVGLNCRLGPAELLRSFENTVIPEDALLSVFPNAGRLG
MTDGEYAFKSSPEYFGQSALRLREQGVRLIGGCCGTTPAHVKAIADALESLEPQARVNPT
IVAGDRPTISVQNTREREKPSIVERVKTATTIIVEFDPPRDLDADQFLHGCCELHKAGAD
AITLADNSLATVRMSNMALGALMKSRHGIDPLLHLACRDRNLIGQQSHLMGLNALGIDQI
LVITGDPSRFGDLPGASSVFDVTSFDLIRMVKQLNEGVSFSGRPLKQKAQFIVGAAFNPN
VRNMDAAVARLEKKVEAGADYIMTQPVYDAESIRNVYEATKHIGIPVFIGIMPLTSSRNA
EFLHNEVPGIKLSTEALERMKKVQGEAARQEGIAIAKELVDTAVRYFNGIYLITPFNYYE
MAAELIQYTRQQSSLVRMAQT

NBRC No.	clone name	start	stop	length(bp)
G10-001-083	BB1FE1L11002A08	2,293,824	2,327,756	33,933

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.