CDS Information : BBR47_28450

Display	1000bp 10000bp 100000bp Clone

Brevibacillus brevis NBRC 100599 chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Brevibacillus brevis NBRC 100599
Replicon	chromosome
Start / Stop / Direction	2,969,670 / 2,970,620 / +
Location	2969670..2970620
Type	CDS
Length	951 bp (316 aa)

Product	D-alanine--D-alanine ligase
Gene name	ddl
Functional category	Cell envelope and cellular processes - Cell wall
EC number	6.3.2.4
Note
KEGG pathway
Sequence feature
References	HAMAP:MF_00047 Uniprot:P07862 Pubmed:1993184

BLASTP Database:UniProtKB:13.0 show BLAST table	1 E=2e-97 A9U7Q2_PHYPA Predicted protein Alignment 2 E=2e-89 Q3EKQ8_BACTI D-alanine--D-alanine ligase Alignment 3 E=7e-88 A9VHL5_9BACI D-alanine--D-alanine ligase Alignment 4 E=1e-87 Q63AX2_BACCZ D-alanine--D-alanine ligase Alignment 5 E=4e-87 A0REK3_BACAH D-alanine--D-alanine ligase Alignment
HAMAP Database:HAMAP:20080521	MF_00047   DDL
InterPro Database:interpro:16.0	IPR000291 D-alanine--D-alanine ligase/VANA/B/C, conserved site (Conserved_site) PS00843   DALA_DALA_LIGASE_1 IPR000291 D-alanine--D-alanine ligase/VANA/B/C (Family) PS00843   DALA_DALA_LIGASE_1 IPR005905 D-alanine--D-alanine ligase (Family) PTHR23132   D-ALANINE--D-ALANINE LIGASE TIGR01205   D_ala_D_alaTIGR: D-alanine--D-alanine ligas IPR011095 D-alanine--D-alanine ligase, C-terminal (Domain) PF07478   Dala_Dala_lig_C IPR011127 D-alanine--D-alanine ligase, N-terminal (Domain) PF01820   Dala_Dala_lig_N IPR011761 ATP-grasp fold (Domain) PS50975   ATP_GRASP IPR013816 ATP-grasp fold, subdomain 2 (Domain) G3DSA:3.30.470.20   no description IPR013817 Pre-ATP-grasp fold (Domain) G3DSA:3.40.50.20   no description IPR016185 PreATP-grasp-like fold (Domain) SSF52440   PreATP-grasp domain
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	316 amino acids
molecular mass	34,520.67 Da
pI	4.66
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 8.5 (27aa) 7 (22aa) 11.4 (36aa) 7.3 (23aa) 1.3 (4aa) 0.9 (3aa) 4.4 (14aa) 3.2 (10aa) 8.9 (28aa) 6.3 (20aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 4.4 (14aa) 1.6 (5aa) 2.5 (8aa) 7 (22aa) 4.1 (13aa) 4.4 (14aa) 1.3 (4aa) 3.8 (12aa) 4.7 (15aa) 7 (22aa)

>BBR47_28450 D-alanine--D-alanine ligase
ATGAAGATAGGCGTTATTATGGGTGGTATTTCTTCGGAGCGTGAGGTTTCTCTGAAGACT
GGTCAAGAGATGATCAATCATTTGGATCGCAGTCGCTATGAGGCCGTTCCTATTGTCATT
ACGCAGCGAGCAGATTTAATCACGCAGGTTCAGCAGGCAGGCATCGATTTCGCACTGTTG
GCGCTGCATGGCCAATATGGTGAGGATGGCACAGTGCAGGGAGCGCTGGAGACACTCGGT
ATCCCCTATACAGGAAGCGGTGTACTGGCAAGCAGCCTATGTATGAATAAGCAACTGTCC
AAGATGCTGCTGAAGACGGCAGGTATTCAAACGCCTGCTGGTCTTTACTGGAAGGACGTT
TATGATCCACAGGCGGTGGAGCAATTGGGCTACCCGGTGATTGTGAAGCCAAATTTGGGG
GGATCCAGCATTGGTGTCCAGCTAGTGCAAAATGAGAAGGAGCTGCTGTCAGCTGTTCAG
GAAGCCAGTTATTTGGATCAGGGGATCTTGATTGAGCCCTATTTGAAAGGGACGGAGCTA
ACCTGCGCGATACTGGATGGCGAGGTACTACCGATTATCGGCATTCGTTCGGCGCATTCA
GAGTGGTTCGACTACCGAGCGAAATATGAGGATGGCGGCGCGGAGGAGAAGGTGATCGAG
CTTCCTCCCGATATCCAGCAGCGAGTGCGAGAGGCGGCCCTCGCCAGCTACCAACTGCTG
CAGTGCAAAGTCTATGCAAGGGTCGATATGATTTTGTGTCAGGATATGCCTTTGTGTCAG
GATATGCCTTACGTACTAGAAGTGAACACCTTACCGGGGATGACCGCTAACAGTCTGTTT
CCAAAGAGTGCAGCAGCAGCAGGTATGACCTTTACACAGGTACTGGACCGTATTATTGCG
AGCTCGCTACATGAGCGGAAACGGGAATGGGGGAACTCTGCAAACGGATAG


>BBR47_28450 D-alanine--D-alanine ligase
MKIGVIMGGISSEREVSLKTGQEMINHLDRSRYEAVPIVITQRADLITQVQQAGIDFALL
ALHGQYGEDGTVQGALETLGIPYTGSGVLASSLCMNKQLSKMLLKTAGIQTPAGLYWKDV
YDPQAVEQLGYPVIVKPNLGGSSIGVQLVQNEKELLSAVQEASYLDQGILIEPYLKGTEL
TCAILDGEVLPIIGIRSAHSEWFDYRAKYEDGGAEEKVIELPPDIQQRVREAALASYQLL
QCKVYARVDMILCQDMPLCQDMPYVLEVNTLPGMTANSLFPKSAAAAGMTFTQVLDRIIA
SSLHERKREWGNSANG

NBRC No.	clone name	start	stop	length(bp)
G10-001-107	BB1FE1L11002H12	2,942,749	2,981,010	38,262

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.