CDS Information : BBR47_40890

Display	1000bp 10000bp 100000bp Clone

Brevibacillus brevis NBRC 100599 chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Brevibacillus brevis NBRC 100599
Replicon	chromosome
Start / Stop / Direction	4,320,334 / 4,319,414 / -
Location	complement(4319414..4320334)
Type	CDS
Length	921 bp (306 aa)

Product	pseudouridine synthase
Gene name
Functional category	Information pathways - RNA modification
EC number	5.4.99.-
Note
KEGG pathway
Sequence feature
References	Uniprot:P33643 Pubmed:11453071,11087118,14501142

BLASTP Database:UniProtKB:13.0 show BLAST table	1 E=3e-67 Q5L1R4_GEOKA Pseudouridine synthase Alignment 2 E=3e-66 A4IL87_GEOTN Pseudouridine synthase Alignment 3 E=2e-65 Q18YC4_DESHD Pseudouridine synthase Alignment 4 E=2e-65 Q24Y13_DESHY Pseudouridine synthase Alignment 5 E=4e-65 Q9K905_BACHD Pseudouridine synthase Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR006145 Pseudouridine synthase (Domain) PD001819   Q9K905_BACHD_Q9K905; PF00849   PseudoU_synth_2 IPR006224 Pseudouridine synthase, Rlu (Conserved_site) PS01129   PSI_RLU IPR006224 Pseudouridine synthase, Rlu (Family) PS01129   PSI_RLU IPR006225 Pseudouridine synthase, RluD (Domain) TIGR00005   rluA_subfam: pseudouridine synthase, RluA f IPR020103 Pseudouridine synthase, catalytic domain (Domain) SSF55120   Pseudouridine synthase
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	306 amino acids
molecular mass	34,514.25 Da
pI	6.52
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 7.5 (23aa) 7.8 (24aa) 8.2 (25aa) 5.2 (16aa) 3.3 (10aa) 1.3 (4aa) 3.9 (12aa) 3.9 (12aa) 8.2 (25aa) 4.9 (15aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 5.6 (17aa) 0.3 (1aa) 3.3 (10aa) 5.2 (16aa) 2.3 (7aa) 4.2 (13aa) 3.3 (10aa) 7.8 (24aa) 4.9 (15aa) 8.8 (27aa)

>BBR47_40890 pseudouridine synthase
ATGCTAGAAGAATTAATTAGCTTCACGGTTGATGAGGCAGATGCAGGACAAACCGTGCGA
GAAGTCCTGCAAAAGCGGTATGGTGTATCGAGGCGTTTGCTCATTCGTGCCAAGTTTAAA
GGCTCCATTACACGGAATGGCGTTCTCGTATTTGTAAACGAAAAATTGCAAGCCGGCGAT
AAAATAGCAGTCATGGTTGAAGAAGAAGCAGAAGAGACTGTTGCTCCAGAGGACATGCCG
CTCTCCATTCGTTACGAGGACGAAGATTTGATGGTCATCGCAAAGCCAGCGGGATTGGTC
GTTCATCCGACAGGAAATCATCCCAGTGGCACTTTGGCAAACGGGATGATCGCTTATTGG
AAAAAGCGTGGCGAGAACAGAAAGTTCCGGGCGGTGAACCGTTTGGATAAGGATACATCA
GGCTTGATGATCGTAGCGAAAAATCAATGGGCGCATGAACAATTCAGCCGCATGCAGCAG
ATCAGGACGTTGCAGCGGACTTATCGGGCTATCGTCCAAGGAATCGTGGAGTCAGACGAC
GGTACAATTAATACGCCGATTGGCCTTGCGGAAAACTCATTTATTACCAGACAGGTAAGG
CCGGATGGGCAGACAGCTGTTACCCATTATCGTGTGCTTGCCCGAGGGGAAGGAATGAGT
TTTGTTGAGGTGAAGCTGGAGACGGGACGTACGCATCAGATTCGCGTACATATGAGCAGT
GAAGGGCACCCACTTGCCGGAGATGATCTGTACGGGGGAGAACGGGAGCATATCGGCAGA
CAAGCTTTGCATGCATGGGGGCTATCCTTTGTTCACCCGCGTAGCGGTCTTGCGATGAGC
TGGGAGGAGCCGCTGCCAAATGATATGGAGCAGCTAGCCCAGCAATTTTTTCCCGATTAT
CTGTGCAAGGAAATGACTTAA


>BBR47_40890 pseudouridine synthase
MLEELISFTVDEADAGQTVREVLQKRYGVSRRLLIRAKFKGSITRNGVLVFVNEKLQAGD
KIAVMVEEEAEETVAPEDMPLSIRYEDEDLMVIAKPAGLVVHPTGNHPSGTLANGMIAYW
KKRGENRKFRAVNRLDKDTSGLMIVAKNQWAHEQFSRMQQIRTLQRTYRAIVQGIVESDD
GTINTPIGLAENSFITRQVRPDGQTAVTHYRVLARGEGMSFVEVKLETGRTHQIRVHMSS
EGHPLAGDDLYGGEREHIGRQALHAWGLSFVHPRSGLAMSWEEPLPNDMEQLAQQFFPDY
LCKEMT

NBRC No.	clone name	start	stop	length(bp)
G10-001-158	BB1FE1L10024D04	4,307,124	4,348,615	41,492

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.