CDS Information : BBR47_48990

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Brevibacillus brevis NBRC 100599 chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Brevibacillus brevis NBRC 100599
Replicon	chromosome
Start / Stop / Direction	5,153,615 / 5,151,207 / -
Location	complement(5151207..5153615)
Type	CDS
Length	2,409 bp (802 aa)

Product	probable 3-hydroxyacyl-CoA dehydrogenase
Gene name	fadN Synonym: yusL
Functional category	Intermediary metabolism - Metabolism of lipids
EC number	1.1.1.35
Note	fadN/yusL in Bacillus subtilis.
KEGG pathway
Sequence feature
References	Family/Domain:IPR006176,IPR006108 Uniprot:O32178 Pubmed:17189250

BLASTP Database:UniProtKB:13.0 show BLAST table	1 E=0 Q2B1N6_9BACI 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase/carnithine racemase fusion Alignment 2 E=0 A7GUI9_BACCN 3-hydroxyacyl-CoA dehydrogenase NAD-binding precursor Alignment 3 E=0 Q5KVJ3_GEOKA 3-hydroxyacyl-CoA dehydrogenase Alignment 4 E=0 Q631W2_BACCZ 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase/carnithine racemase fusion Alignment 5 E=0 Q815X0_BACCR Enoyl-CoA hydratase Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR001753 Enoyl-CoA hydratase/isomerase (Domain) PF00378   ECH IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain) PF00725   3HCDH IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD-binding (Domain) PF02737   3HCDH_N IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179   6-phosphogluconate dehydrogenase C-terminal domain-like IPR013328 Dehydrogenase, multihelical (Domain) G3DSA:1.10.1040.10   no description IPR016040 NAD(P)-binding domain (Domain) SSF51735   NAD(P)-binding Rossmann-fold domains G3DSA:3.40.50.720   no description
SignalP	Bacteria, Gram-positive
TMHMM	Transmembrane 1
SOSUI	No significant hit

size of protein	802 amino acids
molecular mass	88,310.80 Da
pI	6.85
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 8.6 (69aa) 9.2 (74aa) 9.5 (76aa) 4.9 (39aa) 4 (32aa) 0.9 (7aa) 4.1 (33aa) 3.4 (27aa) 8.6 (69aa) 5.2 (42aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 4.5 (36aa) 0.5 (4aa) 4.1 (33aa) 3 (24aa) 2.7 (22aa) 8 (64aa) 2 (16aa) 4.1 (33aa) 4.6 (37aa) 8.1 (65aa)

>BBR47_48990 probable 3-hydroxyacyl-CoA dehydrogenase
ATGGAACGCAAAATTCGCAAGGCGGCAGTTCTCGGTTCAGGCGTCATGGGCGCCGGGATC
GCAGCACATTTAGCCAACGTCGGTATTCCCACTTATTTACTGGACATCGTGCCGCGTGAA
TTGACAGCAGATGAGAGTAATAAAGGACTTTCTTTGTCTGATAGCGTGGTGAAAAACCGG
ATAGCACAAGCGGGCCGAGACCGACTTTTGAAGGAGAAGCCGGCGCCGCTCTACGATAAA
AAGAACATCGACTTGATTACAGTTGGTAACTTCGAGGATCATCTCTCTTGCTTGAGCGAG
GTCGATTGGATTATTGAGGTAGTTGTCGAGAACATCGAAGTGAAGAAAAGCGTCTTCGCT
ATGGTAGAAGCGCATCGCAAGCCAGGGACCATCGTGTCTTCCAATACGTCTGGGGTTTCT
ATCAATGAAATGGCAGAGGGACGCTCGGATGACTTCCGCAAGCATTTCCTCGGCACGCAC
TTTTTTAACCCACCGCGCTACTTGAAGCTGTTGGAGCTGATTCCAGGTCTTGATACAGAC
CCGCAGGTCATCTTTTTCATGAAGCATTTTGGTGAGCATGTGCTCGGAAAAGGAATGGTC
GTTTGCAAGGATACACCGAACTTCATCGCCAATCGGATCGGTACATATGGATTGCAGGTG
TCGATCCACGAAATGGTGCGACTGGGGCTTGGGGTAGATGAGGTAGATGCGTTGACAGGT
CCGGTCATCGGCCGTCCGAAGAGCGCGACGTTCCGCACACTCGATGTCGTGGGACTGGAT
ACGTACGTGCATGTGGCGGGTAACGTCAGGAATAAAAGCACGGATGAGCAGGAGCGCTCT
GTTTTTGAAGTGCCGGAGTTCGTTCTGCAAATGGTGGAGAAAAAATGGATCGGGCAAAAA
GCTGGGCAAGGCTTTTTCAAGCAGGTGAAGACGGAGAAAGGCAAAGAGATTTTGGCACTG
TCGGTTGACACGCTGGAATACCGTCCAAGTGTGAGGCCGAAGTTTGCTTCCCTCGAAGCA
GCCAAAACAGCCAAGACACTGCCTGAGAAGTTGAGAGCGCTCGCATACGGCAAGGACAAG
GGCAGTGAGTTCGTCTGGAGTGTCTTCAAAAAGGTACTGCTGTACTCTGCTGAAAAGGCA
TATGAGATCGCTGACGATATCGTCTCTGTTGACCAGGCCATGAAGTGGGGCTTCGGCTGG
GAGATGGGGCCATTCGAAACGTGGGATGCCATCGGTCTCGAAAAGTCAGTGGCAAAAATG
CGTGAGGAAAACGAGAAGATTCCGGCACTCGTCGAGGAGCTGCTCGCGAGTGGGAAAACC
TCCTTTTATCAAAAAGAGCAAGGGAAGCGCAGCGTGTTTTCCATCGGAGGCACATACAAG
GACGTCGAAGAGAGCAAGGAAAACATCAACCTGGCAGCCCTTAAGGAACAAGGCAAGCTA
ATCAAGAAAAATGCGGGTGCCGCCTTGATTGATTTGGGCGATGGAGTCGCATGTCTGGAA
TTCACCTCTCCGCACAATGCACTCGGGATGGATGTCTTGCACATGGCAAGTCAGGCCGCA
GAGGAAGTGCAGAAGAACTTCGCAGGTCTGGTCATCGGTAATCAAGGCAAAAACTTCTGC
GTCGGGATGAATCTCGCAATGGCATTGATGGAAGCACAGGATGAGAACTGGTTGGAGCTC
GACATGCTCGTCACGAACTTCCACAAAACAGCACGGGCACTGCGCTACATGCATCGTCCA
GTGGTAGCAGCACCATTCGGCATGACGCTGGGTGGCGGTGTCGAGGTGGTCTATCTCGCG
GATCGCGTGCAGGTATCGGCAGAGACGTACCTCGGACTGGTAGAGGTGGGAGTAGGACTT
CTGCCAGGTGGCGGAGGAACGAAGGAGATGCTGTTCCGAGCAATGGAAAACGTTCCAGAG
GGAGGAAGCGTGCCAGTTGATCCGTTCCCATTCGTTGCGAAGGCGTTTGAAGCCATTGCC
ATGGCAAAGGTATCTACGAGTGGTCAGGAAGCCATCAATCTGGGGTACCTGCGACCGACT
GACCGCATCAGCGTGAATGCTGATCATCTGCTATACGATGCGAAGCAGCTGGTGCTTACG
ATGGATAAAGAGGGCTACACGCCGCCAGCGCCACGCAAAATTCGCGTCATCGGTGAAACG
GGTTATGCGAACCTTCGCCAAAATATTTATGCCATGAAGAAGAGTGGATATATTTCCGAG
CATGATGAGCTGATCGCAAGCAAAGTTGCCTATGTTATGTCCGGCGGTAACGTGCCAGCG
GGTACAGAAGTGACAGAGAACTACATTCTGGAGCTGGAAAAGCAAGCGTTCCTCGAACTG
ATCAAGACGCCGAAAAGCCAGCAACGGATGCAGCACATGCTGACCAAGAACAAGCCTTTG
CGTAACTAG


>BBR47_48990 probable 3-hydroxyacyl-CoA dehydrogenase
MERKIRKAAVLGSGVMGAGIAAHLANVGIPTYLLDIVPRELTADESNKGLSLSDSVVKNR
IAQAGRDRLLKEKPAPLYDKKNIDLITVGNFEDHLSCLSEVDWIIEVVVENIEVKKSVFA
MVEAHRKPGTIVSSNTSGVSINEMAEGRSDDFRKHFLGTHFFNPPRYLKLLELIPGLDTD
PQVIFFMKHFGEHVLGKGMVVCKDTPNFIANRIGTYGLQVSIHEMVRLGLGVDEVDALTG
PVIGRPKSATFRTLDVVGLDTYVHVAGNVRNKSTDEQERSVFEVPEFVLQMVEKKWIGQK
AGQGFFKQVKTEKGKEILALSVDTLEYRPSVRPKFASLEAAKTAKTLPEKLRALAYGKDK
GSEFVWSVFKKVLLYSAEKAYEIADDIVSVDQAMKWGFGWEMGPFETWDAIGLEKSVAKM
REENEKIPALVEELLASGKTSFYQKEQGKRSVFSIGGTYKDVEESKENINLAALKEQGKL
IKKNAGAALIDLGDGVACLEFTSPHNALGMDVLHMASQAAEEVQKNFAGLVIGNQGKNFC
VGMNLAMALMEAQDENWLELDMLVTNFHKTARALRYMHRPVVAAPFGMTLGGGVEVVYLA
DRVQVSAETYLGLVEVGVGLLPGGGGTKEMLFRAMENVPEGGSVPVDPFPFVAKAFEAIA
MAKVSTSGQEAINLGYLRPTDRISVNADHLLYDAKQLVLTMDKEGYTPPAPRKIRVIGET
GYANLRQNIYAMKKSGYISEHDELIASKVAYVMSGGNVPAGTEVTENYILELEKQAFLEL
IKTPKSQQRMQHMLTKNKPLRN

NBRC No.	clone name	start	stop	length(bp)
G10-001-189	BB1FE1L10026A09	5,119,141	5,155,081	35,941
G10-001-190	BB1FE1L10020H03	5,142,421	5,181,031	38,611

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.