CDS Information : BBR47_57720

Display	1000bp 10000bp 100000bp Clone

Brevibacillus brevis NBRC 100599 chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Brevibacillus brevis NBRC 100599
Replicon	chromosome
Start / Stop / Direction	6,117,322 / 6,116,762 / -
Location	complement(6116762..6117322)
Type	CDS
Length	561 bp (186 aa)

Product	RNA polymerase ECF-type sigma factor
Gene name
Functional category	Information pathways - RNA synthesis - - Initiation
EC number
Note
KEGG pathway
Sequence feature
References	Uniprot:Q45585 Pubmed:11454200,14993308,12073657

BLASTP Database:UniProtKB:13.0 show BLAST table	1 E=2e-33 Q2BEM6_9BACI RNA polymerase sigma-70 factor Alignment 2 E=5e-25 Q67KL8_SYMTH RNA polymerase ECF-type sigma factor Alignment 3 E=1e-23 Q18R26_DESHD RNA polymerase, sigma-24 subunit, ECF subfamily Alignment 4 E=2e-23 A5V1V8_ROSS1 RNA polymerase, sigma-24 subunit, ECF subfamily Alignment 5 E=4e-23 Q24ZK5_DESHY Putative uncharacterized protein Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR007627 RNA polymerase sigma-70 region 2 (Domain) PF04542   Sigma70_r2 IPR013249 RNA polymerase sigma factor 70, region 4 type 2 (Domain) PF08281   Sigma70_r4_2 IPR013324 RNA polymerase sigma factor, regions 3 and 4 (Domain) SSF88659   Sigma3 and sigma4 domains of RNA polymerase sigma factors IPR013325 RNA polymerase sigma factor, region 2 (Domain) SSF88946   Sigma2 domain of RNA polymerase sigma factors IPR014284 RNA polymerase sigma-70 (Domain) TIGR02937   sigma70-ECF: RNA polymerase sigma factor, s
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	186 amino acids
molecular mass	21,731.88 Da
pI	7.54
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 9.1 (17aa) 7 (13aa) 8.6 (16aa) 3.2 (6aa) 4.3 (8aa) 1.6 (3aa) 3.8 (7aa) 3.8 (7aa) 3.2 (6aa) 4.8 (9aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 3.8 (7aa) 2.2 (4aa) 4.8 (9aa) 6.5 (12aa) 5.4 (10aa) 7 (13aa) 3.2 (6aa) 5.4 (10aa) 2.7 (5aa) 9.7 (18aa)

>BBR47_57720 RNA polymerase ECF-type sigma factor
ATGGAGGAGGATCAAGAGTGGGTGAAGCAAGTCTTGGGAGGCGACCCGCATGCCTTTGCC
CACCTTGTGAACAAGTACAAGTACAGGGTGTGCTCGTTACTCGTACGGATGGGCAATTCC
CCAGAGGATGCAGAGGATTTTGCACAGACTGCTTTCGTCAATGCGTATAAAAGCTTGCAG
CAATATGATGCGAGCCGTCCGTTTTCAGCTTGGTTAAATAAAATCGCAATCAACTGTTGT
CTGCAAGCATGGCGGACGGCACAGAAGAAGAATCCGATCGTGCCTTTTCAAGAATCCATG
AAGGCGCACACCAATTATGAAAACCCGGAGTCGTTTTACCTGGCTCGTGAAAAACAAAAC
GAAATATTCGCTGTCATGGGAGAACTCGCTTCCCATTACCGGATCGTGCTTACTCTACGA
TATTTTGAAGAGCTGAGCTATCAAGAAATTGCTGAGACGATGCAAGTGCCGGTAACTACC
GTGCAAGTTCACCTGTACCGCGCGAAGAAAAAGCTCCGTGAGCTCATGGGGAAAAGGGGG
GAACTTCATGAAATGTGCTGA


>BBR47_57720 RNA polymerase ECF-type sigma factor
MEEDQEWVKQVLGGDPHAFAHLVNKYKYRVCSLLVRMGNSPEDAEDFAQTAFVNAYKSLQ
QYDASRPFSAWLNKIAINCCLQAWRTAQKKNPIVPFQESMKAHTNYENPESFYLAREKQN
EIFAVMGELASHYRIVLTLRYFEELSYQEIAETMQVPVTTVQVHLYRAKKKLRELMGKRG
ELHEMC

NBRC No.	clone name	start	stop	length(bp)
G10-001-226	BB1FE1L10007D09	6,078,307	6,119,734	41,428
G10-001-227	BB1FE1L10035C06	6,108,547	6,146,130	37,584

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.