CDS Information : DMR_02480

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Desulfovibrio magneticus RS-1 (= NBRC 104933) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Desulfovibrio magneticus RS-1 (= NBRC 104933)
Replicon	chromosome
Start / Stop / Direction	265,005 / 266,762 / +
Location	265005..266762
Type	CDS
Length	1,758 bp (585 aa)

Product	Fe hydrogenase
Gene name
Functional category	Metabolism - Energy Metabolism
EC number	1.12.7.2
Note	Contains one 4Fe-4S ferredoxin, iron-sulfur binding domain (IPR001450). Contains two Ferredoxin domain (IPR001041). Contains three Iron hydrogenase, small subunit domain (IPR003149). Contains four Hydrogenase large subunit, C-terminal domain (IPR004108).
KEGG pathway	map:00680 KO:K00532
Sequence feature
References	Family/Domain:IPR003149 Uniprot:P29166 Pubmed:9836629

BLASTP Database:UniProtKB:14.0 show BLAST table	1 E=0 Q46508_DESFR Potential NAD-reducing hydrogenase subunit Alignment 2 E=0 Q3A430_PELCD NAD-reducing iron-only hydrogenase large subunit Alignment 3 E=0 Q3A458_PELCD NAD-reducing iron-only hydrogenase large subunit Alignment 4 E=0 A7M653_BACOV Putative uncharacterized protein Alignment 5 E=0 Q8ABI6_BACTN NADH:ubiquinone oxidoreductase subunit Alignment
HAMAP	No significant hit
InterPro Database:interpro:18.0	IPR001041 Ferredoxin (Domain) PF00111   Fer2 PS51085   2FE2S_FER_2 SSF54292   2Fe-2S ferredoxin-like IPR001450 4Fe-4S ferredoxin, iron-sulfur binding (Conserved_site) PS00198   4FE4S_FER_1 IPR001450 4Fe-4S ferredoxin, iron-sulfur binding (Domain) PF00037   Fer4 PS00198   4FE4S_FER_1 IPR003149 Iron hydrogenase, small subunit-like (Domain) PF02256   Fe_hyd_SSU G3DSA:4.10.260.20   no description IPR004108 Hydrogenase large subunit, C-terminal (Domain) PF02906   Fe_hyd_lg_C IPR009016 Iron hydrogenase (Domain) SSF53920   Fe-only hydrogenase IPR013352 Hydrogenase, Fe-only (Domain) TIGR02512   Fe_only_hydrog: hydrogenases, Fe-only IPR017896 4Fe-4S ferredoxin, iron-sulpur binding domain (Domain) PS51379   4FE4S_FER_2
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	585 amino acids
molecular mass	63,772.39 Da
pI	5.76
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 9.1 (53aa) 8 (47aa) 9.7 (57aa) 5.3 (31aa) 3.2 (19aa) 0.3 (2aa) 5 (29aa) 2.9 (17aa) 8.2 (48aa) 4.1 (24aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 6 (35aa) 3.6 (21aa) 3.1 (18aa) 2.4 (14aa) 2.2 (13aa) 5.3 (31aa) 2.6 (15aa) 5.6 (33aa) 7.4 (43aa) 6 (35aa)

>DMR_02480 Fe hydrogenase
ATGTCCATGCTGACAGTGCATATAGACGGCAAGACCACCACCATCCCGGCCGGCGGCACC
ATCCTGGACGCCGCCCGCAAACTGGATATCGACATCCCGACCCTGTGCTACCTCAATCTT
GAGGATCTCAAGGTCAACAACAAGGCCGCCTCCTGCCGCATCTGCGTGGTGGAGGTCGAG
GGTCGGCGCAACCTGGCTCCGGCCTGCGCCACCCCGGCCACCGACGGCATGGTGGTCAAG
TCCAACACCCTGCGCGTCCTTAACGCCCGTAAGACCGTGCTGGAGCTGCTGCTCTCCGAC
CACCCCAAGGACTGCCTCGTGTGCGCCAAGTCCGGCGAATGCGAGCTGCAGGATCTGGCC
GAAAAGTTCGGCATCCGCGAGTCGCCCTATGACGGCGGCGAAATGTCCCACTACCGCAAG
GACGTCTCGCCCTCCATCATCCGCGACATGGACAAGTGCATCATGTGCCGCCGCTGCGAG
ACCATGTGCAACGACATCCAGACCTGCGGCGTGCTGTCGGGCGTCAACCGCGGCTTCACC
GCCGTGGTCGCCCCGGCCTTCGAGATGAACCTGGCCGACACCGTGTGCACCAACTGCGGC
CAGTGCGTGGCCGTGTGCCCGGTGGGCGCGCTGGTGGAAAACGACAACAGCTGGGACGTG
GTCGACGCCCTGGCCGACCCGGACAAGGTGGTCATCGTCCAGACCGCCCCGGCCGTGCGC
GCCGCCCTGGGCGAAGACCTCGGCATCGCCCCGGGCACGTCCGTCACCGGCAAGATGGCC
GCCGCCCTGCGCCGCCTGGGCTTTGACCACATCTTCGACACCGACTTCGCCGCCGACCTC
ACCATCATGGAAGAAGGCTCGGAGTTCCTCGACCGCCTGACCCGCTACCTGGGTGGCGAC
CAGACGGCCAAGCTGCCCATCCTGACCTCCTGCTGTCCCGGCTGGGTCAAGTTCTTCGAG
CACAACTTCCAGGACATGCTCGACGTGCCTTCCACCGCCAAGTCGCCCCAGCAGATGTTC
GGCGCCATCGCCAAGACCTACTACGCCGACATGCTGGGCATCCCCCGCGACAAGCTGGTC
GTGGTCTCGGTCATGCCGTGCCTGGCCAAGAAGTACGAACGCGCCCGGCCGGAGTTCTCC
GTGGACGGCAACCCGGACGTGGACATCGTCATCTCCACCCGTGAGCTGGCCCGGCTGATC
AAGCGCATGAACATCGACTTCGCCAGCCTGCCTGACGAAGACTTCGACGCGCCCCTTGGC
GAGTCCACCGGCGCGGCTCCGATCTTCGGCGTCACCGGCGGCGTCATCGAGGCGGCCCTG
CGCACCGCTTACGAGCTGGCCACCGGCGAAACCCTCCAGAAGGTCGACTTCGAGGACGTG
CGCGGCATGGACGGCGTCAAGGTGGCCACGGTCCAGGTCGGCCCCCACGAACTGCGCATC
GGCATCGCCCATGGCCTGGGCAACGCCCGCAAGCTCCTTAACCGGGTGCGCGAAGGCGAA
ACCTTCCACGCCATCGAGGTCATGGCCTGCCCCGGCGGCTGCATCGGCGGCGGCGGACAG
CCCTACCACCACGGTGACATTGAGCTGCTCAAGCTGCGCACCCAGGTGCTTTACGCCGAA
GACGCCGGCAAGCCGCTGCGCAAGTCGCACCAGAACCCGTACATCATTGAACTGTACGAA
AAGTTCCTGGGCAAGCCGCTGTCCGAGAAGTCACACCACCTGCTGCACACCCACTACTTC
AAGCGCCAGCGCCTGTAG


>DMR_02480 Fe hydrogenase
MSMLTVHIDGKTTTIPAGGTILDAARKLDIDIPTLCYLNLEDLKVNNKAASCRICVVEVE
GRRNLAPACATPATDGMVVKSNTLRVLNARKTVLELLLSDHPKDCLVCAKSGECELQDLA
EKFGIRESPYDGGEMSHYRKDVSPSIIRDMDKCIMCRRCETMCNDIQTCGVLSGVNRGFT
AVVAPAFEMNLADTVCTNCGQCVAVCPVGALVENDNSWDVVDALADPDKVVIVQTAPAVR
AALGEDLGIAPGTSVTGKMAAALRRLGFDHIFDTDFAADLTIMEEGSEFLDRLTRYLGGD
QTAKLPILTSCCPGWVKFFEHNFQDMLDVPSTAKSPQQMFGAIAKTYYADMLGIPRDKLV
VVSVMPCLAKKYERARPEFSVDGNPDVDIVISTRELARLIKRMNIDFASLPDEDFDAPLG
ESTGAAPIFGVTGGVIEAALRTAYELATGETLQKVDFEDVRGMDGVKVATVQVGPHELRI
GIAHGLGNARKLLNRVREGETFHAIEVMACPGGCIGGGGQPYHHGDIELLKLRTQVLYAE
DAGKPLRKSHQNPYIIELYEKFLGKPLSEKSHHLLHTHYFKRQRL

NBRC No.	clone name	start	stop	length(bp)
G11-001-010	DM1GRC2E00037C06	239,670	279,698	40,029
G11-001-011	DM1GRC2E00016H04	254,674	287,618	32,945

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.