CDS Information : DMR_12950

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Desulfovibrio magneticus RS-1 (= NBRC 104933) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Desulfovibrio magneticus RS-1 (= NBRC 104933)
Replicon	chromosome
Start / Stop / Direction	1,404,460 / 1,404,086 / -
Location	complement(1404086..1404460)
Type	CDS
Length	375 bp (124 aa)

Product	Fe hydrogenase small subunit
Gene name
Functional category	Metabolism - Energy Metabolism
EC number	1.12.7.2
Note	Contains one Iron hydrogenase, small subunit domain (IPR003149). Contains two Fe-only hydrogenase, small subunit domains (IPR008953). Similar to Periplasmic [Fe] hydrogenase small subunit [precursor] in Desulfovibrio vulgaris (P13628).
KEGG pathway	KO:K00532
Sequence feature
References	Family/Domain:IPR008953 Family/Domain:IPR003149

BLASTP Database:UniProtKB:14.0 show BLAST table	1 E=1e-49 PHFS_DESVO Periplasmic [Fe] hydrogenase small subunit precursor Fe hydrogenlyase small chain Alignment 2 E=2e-43 PHFS_DESVH Periplasmic [Fe] hydrogenase small subunit precursor Fe hydrogenlyase small chain Alignment 3 E=2e-43 A1VD87_DESVV Ferredoxin hydrogenase precursor Alignment 4 E=8e-42 O08312_DESFR Cytochrome-c3 hydrogenase precursor Alignment 5 E=2e-36 Q317L3_DESDG Ferredoxin hydrogenase precursor Alignment
HAMAP	No significant hit
InterPro Database:interpro:18.0	IPR003149 Iron hydrogenase, small subunit-like (Domain) PF02256   Fe_hyd_SSU G3DSA:4.10.260.20   no description IPR006311 Twin-arginine translocation pathway signal (Conserved_site) TIGR01409   TAT_signal_seq: Tat (twin-arginine transloc IPR006311 Twin-arginine translocation pathway signal (Domain) TIGR01409   TAT_signal_seq: Tat (twin-arginine transloc IPR008953 Fe-only hydrogenase, small subunit (Domain) SSF48674   Fe-only hydrogenase smaller subunit IPR017909 Twin arginine translocation signal, Tat (Domain) PS51318   TAT
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	124 amino acids
molecular mass	13,925.00 Da
pI	10.2
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 12.1 (15aa) 2.4 (3aa) 7.3 (9aa) 4 (5aa) 4.8 (6aa) 0.8 (1aa) 4 (5aa) 4.8 (6aa) 8.1 (10aa) 3.2 (4aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 7.3 (9aa) 0.8 (1aa) 4 (5aa) 5.6 (7aa) 4 (5aa) 8.9 (11aa) 3.2 (4aa) 5.6 (7aa) 4 (5aa) 4.8 (6aa)

>DMR_12950 Fe hydrogenase small subunit
ATGACTTTCATCGCTTCGACCCGTCGCGGCTTCCTCAAAGCCGCCTGCATCATGACCGGC
GGCGCCCTCATCGGGCTTCGCATGACCGGCAAGGCCGTGGCCGCCGCCAAGCAACTCAAG
GACTTCATGATGGACCGCATCGACGGCGTCTACGGGGCCGACGCCAAGTTCCCCGAGCGG
GCCTCTCAGCAAAACGGCCAGGTCCAGACCCTGTACAAGAACTTCCTGGAACATCCCATG
AGCCACAAGGCCGAGCAGCTCCTGCACACCCATTGGGAAGACCGCTCCAAGAACATCAAA
AAACTCCAGACCGCCGGCAACTACCCCAACCCCCGGGCCAAGGAATTCGCCGGAACCACC
TACCCCTACGAATAG


>DMR_12950 Fe hydrogenase small subunit
MTFIASTRRGFLKAACIMTGGALIGLRMTGKAVAAAKQLKDFMMDRIDGVYGADAKFPER
ASQQNGQVQTLYKNFLEHPMSHKAEQLLHTHWEDRSKNIKKLQTAGNYPNPRAKEFAGTT
YPYE

NBRC No.	clone name	start	stop	length(bp)
G11-001-054	DM1GRC2E00013F07	1,394,395	1,431,063	36,669

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.