CDS Information : KRH_04780

Display	1000bp 10000bp 100000bp Clone

Kocuria rhizophila DC2201 (= NBRC 103217) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Kocuria rhizophila DC2201 (= NBRC 103217)
Replicon	chromosome
Start / Stop / Direction	522,487 / 523,623 / +
Location	522487..523623
Type	CDS
Length	1,137 bp (378 aa)

Product	putative glutamate--cysteine ligase
Gene name
Functional category	Metabolism - Amino Acid Metabolism
EC number	6.3.2.2
Note
KEGG pathway	KO:K01919
Sequence feature
References	Family/Domain:IPR006336 Uniprot:P77213 Pubmed:15211520

UniProt	B2GH73
INSD	BAG28825.1 (DDBJ GenBank EMBL)
UniParc	UPI0001733FAB

BLASTP Database:UniProtKB:12.5 show BLAST table	1 E=1e-57 CAAL2_STRAW Carboxylate-amine ligase SAV_999 Alignment 2 E=2e-44 CAAL1_NOCFA Carboxylate-amine ligase nfa27300 Alignment 3 E=1e-43 CAAL2_MYCVP Carboxylate-amine ligase Mvan_4929 Alignment 4 E=7e-43 CAAL2_MYCSJ Carboxylate-amine ligase Mjls_4754 Alignment 5 E=7e-43 CAAL2_MYCSK Carboxylate-amine ligase Mkms_4460 Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR006336 Glutamate--cysteine ligase, GCS2 (Family) PF04107   GCS2 IPR011793 Enzymatic protein of unknown function (Family) TIGR02050   gshA_cyan_rel: uncharacterized enzyme
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	378 amino acids
molecular mass	41,121.41 Da
pI	4.58
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 15.3 (58aa) 6.6 (25aa) 8.7 (33aa) 4.5 (17aa) 2.9 (11aa) 2.1 (8aa) 6.6 (25aa) 3.2 (12aa) 7.1 (27aa) 5.3 (20aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 5 (19aa) 0.5 (2aa) 1.3 (5aa) 3.4 (13aa) 2.1 (8aa) 0.5 (2aa) 2.4 (9aa) 8.2 (31aa) 7.4 (28aa) 6.6 (25aa)

>KRH_04780 putative glutamate--cysteine ligase
GTGACGCCACCGGCCACCCCACGAGACCAGCAAGGCGGTAGCACCATGAGCACGTTCGGC
ATCGAGGAGGAGTTCCTCCTGATCGACCGCGGGGACAGGCTGCCCGCACGCCCCACTCCG
GAGCAGTCCGCGCAGCTGGTCTCCCTCGAGGCGGGCGGCGGTTCCGCCACGCCCGAGTGG
CTGGCGTGCCAGGTGGAGGAGACCTCGCCGATCTTCCACGACGCCGCCGGGGCCGTGGAC
TCGCTCGTGACCTTCCGCACCGCCCTGCACGACGCCACGGACGCCATGGGCATGGACGTG
GTGGGACTCGCCGCGGCCCCCGACATCCGCCCGGAACCGGCGGACATCACCGTGAACGAG
CGCTACCAGCGCCTCGCCGGGTACACCCCGGCGGTCGCCGCGGACCAGTACATCAGCGGG
ATGCACGTGCACCTGGGCTTCCCGGACATGGAGGACGCTGTCCGTGCGCTGAACGGGCTG
CGCGGCTGGCTGCCCGCGCTCGTGGCGATCGGTGCCAACTCGCCGCTGTGGCGCGGCGCG
GAGTCCGGGTTCGAGTCCTGGCGCTCGATCCACTACCGCCGCTGGATTGCCAACGACATC
CCTCCGCACTTCAAGGACCTGGCGGACCACGAGCAGCGCGTGGCCGTGCTCAGCGCGTTC
GACGTGATGGAGAGCCCCTCCACCCTGAGCTGGCTGGCCCGGCTGTCCCACAAGCACGGC
ACCCTGGAGATCCGGGCGTGCGACGTGCAGCTCGAGGCGCAGGACTCCGTGGCCATCGCG
GTGCTGATCCGCGCCCTGGCGGACTACGCAATGGACGTCCCGCCCACCATGGAGTTCAGT
CAGGAGTACCTGGACATCGCCCTGTGGCAGGCGGCCCGCTGGGGGCTCAGCGGCCGGGTG
CTGGACCCCGTGGCCGCCGAGCTGGTCCCGGGCGAGGCGCTGGTCACCGACCTGCTGGAG
CGGGTGCGGGACTACTACCCCACGGCGGCGGACCGCGAGTTCGCCGAGGCCGGGGTCCGC
CGGATCGCGGAGCGCGGCAACGGGGCCATCCGGCAGCGCCGCGCCTTCGCCCGCGCGGGG
ATCCCCGGTGTCATGGACATGGCCACCGCGGCGATGACGGCCGACCCGCGGGCCTGA


>KRH_04780 putative glutamate--cysteine ligase
MTPPATPRDQQGGSTMSTFGIEEEFLLIDRGDRLPARPTPEQSAQLVSLEAGGGSATPEW
LACQVEETSPIFHDAAGAVDSLVTFRTALHDATDAMGMDVVGLAAAPDIRPEPADITVNE
RYQRLAGYTPAVAADQYISGMHVHLGFPDMEDAVRALNGLRGWLPALVAIGANSPLWRGA
ESGFESWRSIHYRRWIANDIPPHFKDLADHEQRVAVLSAFDVMESPSTLSWLARLSHKHG
TLEIRACDVQLEAQDSVAIAVLIRALADYAMDVPPTMEFSQEYLDIALWQAARWGLSGRV
LDPVAAELVPGEALVTDLLERVRDYYPTAADREFAEAGVRRIAERGNGAIRQRRAFARAG
IPGVMDMATAAMTADPRA

NBRC No.	clone name	start	stop	length(bp)
G09-002-015	MDFO1S8503K01	497,769	537,224	39,456

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.