CDS Information : KRH_10500

Display	1000bp 10000bp 100000bp Clone

Kocuria rhizophila DC2201 (= NBRC 103217) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Kocuria rhizophila DC2201 (= NBRC 103217)
Replicon	chromosome
Start / Stop / Direction	1,146,559 / 1,145,342 / -
Location	complement(1145342..1146559)
Type	CDS
Length	1,218 bp (405 aa)

Product	putative steroid dehydrogenase
Gene name
Functional category	Metabolism - Biosynthesis of Secondary Metabolites and Biodegradation of Xenobiotics
EC number
Note
KEGG pathway
Sequence feature
References	Uniprot:Q03704 Pubmed:1854198

UniProt	B2GFM4
INSD	BAG29397.1 (DDBJ GenBank EMBL)
UniParc	UPI0001733F81

BLASTP Database:UniProtKB:12.5 show BLAST table	1 E=1e-102 A1R683_ARTAT Putative dehydrogenase Alignment 2 E=1e-100 A5CSZ8_CLAM3 Putative NAD(P)H steroid dehydrogenase/isomerase Alignment 3 E=1e-95 A6W8M7_KINRA NAD-dependent epimerase/dehydratase Alignment 4 E=1e-82 A4A9J4_9GAMM Oxidoreductase Alignment 5 E=2e-62 Q0YLU1_9DELT NAD-dependent epimerase/dehydratase:Short-chain dehydrogenase/reductase SDR:3-beta hydroxysteroid dehydrogenase/isomerase:Polysaccharide biosynthesis protein CapD:dTDP-4-dehydrorhamnose reductase:NmrA-like Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370   Epimerase IPR001509 NAD-dependent epimerase/dehydratase (Family) PF01370   Epimerase IPR012272 Bifunctional 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase (Family) PIRSF036679   Bifunctional 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase IPR016040 NAD(P)-binding domain (Domain) SSF51735   NAD(P)-binding Rossmann-fold domains G3DSA:3.40.50.720   no description
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	405 amino acids
molecular mass	43,154.22 Da
pI	5.6
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 12.6 (51aa) 10.6 (43aa) 7.9 (32aa) 2.2 (9aa) 1.7 (7aa) 1.5 (6aa) 5.2 (21aa) 1.7 (7aa) 9.4 (38aa) 6.2 (25aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 6.9 (28aa) 0.5 (2aa) 2 (8aa) 3.5 (14aa) 1 (4aa) 2.2 (9aa) 3 (12aa) 8.4 (34aa) 7.9 (32aa) 5.7 (23aa)

>KRH_10500 putative steroid dehydrogenase
GTGCAGACCGCGGACCCGGACCGCGGCGGTGCCACGGTGACCCACGGCGAGCTCGACGGC
ACGGGCCGCACCGTCCTGGTGACGGGCGCGTCCGGGATGCTCGGCGGCGACGTCGCCCGC
GTGCTGCTCGCGCGCGGCTGGAAGGTGCGCGTGTTCCAGCGCGGCCACGCTCCCGTGGCG
GAGCGCGAGGGCGTGGACGAGGTGCTCGGCTCGGTGACCGATCCCGAGGATGTGGCCCTC
GCACTGGACGGCGTGGACGACGTCGTGCACCTGGCCGCCAAGGTCTCCTTCACCGGGGAC
TGGTCCGAGTTCGTGCGCGTCAACGTCACGGGCACGCGGATCCTGCTGTGCCAGGCGCAG
AACGCCGGGGTGAAGAACGTGGTGTTCGTGTCCTCCCCCTCCGTGGCCCACACCGGGGTC
TCGATCGAGGGCGCGGGTGCGGAGCCCGCGGACCCGCAGAAGGCGCGCGGCAACTACGCC
CGGTCCAAGGCCGCGGCCGAGCTGCTGGCCCTGAGCATGGACGGGCAGCGGGACCTCAAG
GTGGCCGCCGTGCGTCCGCACATCGTGTGGGGCCCCGGGGACACGCAGCTCGTGGAGCGC
GTGGCGGACCGTGCGGCCGCCGGGCGCATGCCCAACCTGGACCACGGTGCCGCGATGATC
GACACCACGTACATCGACAACGCCTCCGAGGGCATCGTCCGGGCGCTGCAGCGCATCGAC
CACGTGCACGGCCGGGCCCTGGTGCTCACCAACGGCGAGCCGCGCCCCGTGGGCGAGCTG
ATCGCCCGGATGTGCCAGGCCACCGGCTCCCCCGCGCCCACCCGATCGGTCCCGGGCAAG
GCGGCACGCCTCGCGGGACGCGTGATCGAGACCGTGTGGCCGCACCTGCCTGCCAAGCTG
CGCGCGGGCGACGGCGAGCCGCCCATGACCGAGTTCCTCGCCGAGCAGCTGTCCACCGCG
CACTGGTTCGACCAGCGCGAGACCCGTGAGCTGCTGGACTGGACGCCGTCCGTGAGCCTG
GACGAGGGCTTCTCGCGCCTGGCCGCGTACTACCAGGACCACCCGCGCCCCAGCAAGGAC
ACCGGCTCCACGTCCCTGGACCGCGTGGAGGAAGTGGCTGACGACCAGCGCGCCCGCGCG
GAGGACTCCTCCGTGGCCTCGGACCGCACGACCGGCAATGCCACCACGGACCTGCAGAGC
GTGCGCTCGGTGCACTGA


>KRH_10500 putative steroid dehydrogenase
MQTADPDRGGATVTHGELDGTGRTVLVTGASGMLGGDVARVLLARGWKVRVFQRGHAPVA
EREGVDEVLGSVTDPEDVALALDGVDDVVHLAAKVSFTGDWSEFVRVNVTGTRILLCQAQ
NAGVKNVVFVSSPSVAHTGVSIEGAGAEPADPQKARGNYARSKAAAELLALSMDGQRDLK
VAAVRPHIVWGPGDTQLVERVADRAAAGRMPNLDHGAAMIDTTYIDNASEGIVRALQRID
HVHGRALVLTNGEPRPVGELIARMCQATGSPAPTRSVPGKAARLAGRVIETVWPHLPAKL
RAGDGEPPMTEFLAEQLSTAHWFDQRETRELLDWTPSVSLDEGFSRLAAYYQDHPRPSKD
TGSTSLDRVEEVADDQRARAEDSSVASDRTTGNATTDLQSVRSVH

NBRC No.	clone name	start	stop	length(bp)
G09-002-028	MDFO1S8502F18	1,110,843	1,154,649	43,807
G09-002-029	MDFO1S8503I17	1,129,364	1,163,679	34,316

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.