CDS Information : KRH_14640

Display	1000bp 10000bp 100000bp Clone

Kocuria rhizophila DC2201 (= NBRC 103217) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Kocuria rhizophila DC2201 (= NBRC 103217)
Replicon	chromosome
Start / Stop / Direction	1,653,680 / 1,654,615 / +
Location	1653680..1654615
Type	CDS
Length	936 bp (311 aa)

Product	cysteine synthase Synonym: O-acetylserine sulfhydrylase Synonym: O-acetylserine (thiol)-lyase
Gene name	cysK
Functional category	Metabolism - Amino Acid Metabolism
EC number	2.5.1.47
Note
KEGG pathway	map:00272 KO:K01738
Sequence feature
References	Uniprot:P37887 Pubmed:11445163

UniProt	B2GJN0
INSD	BAG29811.1 (DDBJ GenBank EMBL)
UniParc	UPI00017345EE

BLASTP Database:UniProtKB:12.5 show BLAST table	1 E=1e-134 A0JXQ5_ARTS2 Cysteine synthase Alignment 2 E=1e-132 A1R7D3_ARTAT Cysteine synthase A Alignment 3 E=1e-125 A5CNP2_CLAM3 Cysteine synthase Alignment 4 E=1e-123 Q4JXA8_CORJK Cysteine synthase Alignment 5 E=1e-120 Q8FMQ7_COREF Cysteine synthase Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR001216 Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site (Binding_site) PS00901   CYS_SYNTHASE IPR001926 Pyridoxal phosphate-dependent enzyme, beta subunit (Domain) SSF53686   Tryptophan synthase beta subunit-like PLP-dependent enzymes PF00291   PALP IPR005856 Cysteine synthase K/M (Family) TIGR01136   cysKM: cysteine synthases IPR005859 Cysteine synthase A (Family) TIGR01139   cysK: cysteine synthase A
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	311 amino acids
molecular mass	32,996.18 Da
pI	4.35
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 9.6 (30aa) 11.6 (36aa) 7.1 (22aa) 6.1 (19aa) 1.6 (5aa) 0.6 (2aa) 4.2 (13aa) 1.9 (6aa) 11.6 (36aa) 4.2 (13aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 6.8 (21aa) 0.3 (1aa) 4.2 (13aa) 2.6 (8aa) 3.2 (10aa) 4.2 (13aa) 0.6 (2aa) 5.1 (16aa) 7.4 (23aa) 7.1 (22aa)

>KRH_14640 cysteine synthase
ATGGGCACCATCTACAACGACGTCACGGAGATCGTGGGGGGCACCCCCCTGGTCCGGCTG
AACCGGCTCGACAACGACCTCCCCGGCAACGTGGCCGTCAAGCTCGAGTTCTACAACCCC
GCCAACTCCGTGAAGGACCGCATCGGCAAGGCCATCGTGGACGCGGCCGAGGCCTCCGGC
CAGCTCAAGCCCGGCGGCACGATCGTGGAGGGCACCTCCGGCAACACCGGCATCGCGCTC
GCCATGGTGGGTGCGGCCCGCGGCTACAAGGTCATCCTGACCATGCCGGAGACCATGTCC
ACCGAGCGTCGCGTCATGCTGCGCGCCTACGGCGCGGAGATCGTGCTGACCCCCGGCGCG
GACGGCATGCGCGGCGCGGTGGACAAGGCCCAGGAGATCGTGGACACCACCGAGAACGCG
GTGCTCGCGAGCCAGTTCGCCAACCCCGCGAACCCCCAGGTGCACTACGAGACCACGGGT
CCGGAGGTCTGGGAGGACACGAACGGGCAGGTGGACGTCTTTGTGGCGGGCGTCGGCACC
GGTGGCACCATCTCCGGCGTCGGGAAGTACCTGCGCGAGCAGAAGTCGGACGTGCGCCTC
ATCGCCGTGGAGCCCTCCGACTCCCCGCTGCTCACCGAGGGCAAGGCCGGGCCCCACAAG
ATCCAGGGCCTCGGCGCCAACTTCGTGCCGGACGTCCTGGACCGCGAGGTCTACGACGAC
GTCTACGACGTCACCGTGGAGGACTCCGTGAGGGTCGCACGCGACCTCGGCACCAAGGAG
GGCATTCTCGGAGGCATCTCTTCCGGCGCCATCGTGTGGGCCGCCCTGCAGGAGGCTGCG
AAGGACGAGAACCGCGACAAGCTGATCGTGGCCGTGGTCTGCGACTTCGGCGAGCGCTAC
ATCTCCACCATCCTCTACGAGGACATCCGGGGCTGA


>KRH_14640 cysteine synthase
MGTIYNDVTEIVGGTPLVRLNRLDNDLPGNVAVKLEFYNPANSVKDRIGKAIVDAAEASG
QLKPGGTIVEGTSGNTGIALAMVGAARGYKVILTMPETMSTERRVMLRAYGAEIVLTPGA
DGMRGAVDKAQEIVDTTENAVLASQFANPANPQVHYETTGPEVWEDTNGQVDVFVAGVGT
GGTISGVGKYLREQKSDVRLIAVEPSDSPLLTEGKAGPHKIQGLGANFVPDVLDREVYDD
VYDVTVEDSVRVARDLGTKEGILGGISSGAIVWAALQEAAKDENRDKLIVAVVCDFGERY
ISTILYEDIRG

NBRC No.	clone name	start	stop	length(bp)
G09-002-041	MDFO1S8502N10	1,627,233	1,658,782	31,550
G09-002-042	MDFO1S8503E21	1,649,995	1,692,514	42,520

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.