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CDS Information : KRH_14860

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close this sectionGenomic map

Display Clone

Kocuria rhizophila DC2201 (= NBRC 103217) chromosome

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Overview 
Genome
Clone

close this sectionLocation

Organism Kocuria rhizophila DC2201 (= NBRC 103217)
Replicon chromosome
Start / Stop / Direction 1,681,348 / 1,679,870 / -
Location complement(1679870..1681348)
Type CDS
Length 1,479 bp (492 aa)

close this sectionAnnotation

Product UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
Gene name murF
Functional category Environmental Information and Cellular Processing
- Cellular Processes
EC number 6.3.2.10
Note
KEGG pathway map:00300 KO:K01929
Sequence feature
References Uniprot:P11880 Pubmed:9166795,2186811,20545602,20133108

close this sectionRelated links to external database

UniProtB2GJQ2
INSDBAG29833.1 (DDBJ GenBank EMBL)
UniParcUPI000173463A

close this sectionComputational search results

BLASTP
Database:UniProtKB:12.5
1E=1e-141A0JV90_ARTS2UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseAlignment
2E=1e-139A1R5F4_ARTATUDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseAlignment
3E=1e-113Q9S2W8_STRCOUDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseAlignment
4E=1e-113Q82AE0_STRAWUDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseAlignment
5E=1e-113A5CS55_CLAM3UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseAlignment
HAMAP No significant hit
InterPro
Database:interpro:16.0
IPR000713 Cytoplasmic peptidoglycan synthetase, N-terminal (Domain)
 [27-103]  3.8e-16 PF01225
PF01225   Mur_ligase
IPR004101 Mur ligase, C-terminal (Domain)
 [333-486]  1.4e-30 G3DSA:3.90.190.20
G3DSA:3.90.190.20   no description
 [334-481]  2.7e-33 SSF53244
SSF53244   MurD-like peptide ligases, peptide-binding domain
 [334-422]  2.2e-13 PF02875
PF02875   Mur_ligase_C
IPR005863 UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanyl ligase (Family)
 [117-482]  1.6e-86 PTHR23135:SF3
PTHR23135:SF3   UDP-N-ACETYLMURAMOYLALANYL-D-GLUTAMYL-2,6-DIAMINOPIMELATE--D-ALANYL-D- ALANYL LIGASE
 [33-480]  6e-124 TIGR01143
TIGR01143   murF: UDP-N-acetylmuramoyl-tripeptide--D-al
IPR012237 UDP-N-acetylmuramate-alanine ligase (Family)
 [9-491]  3.8e-60 PIRSF001562
PIRSF001562   UDP-N-acetylmuramate-alanine ligase
IPR013221 Mur ligase, central (Domain)
 [111-332]  2.4e-51 SSF53623
SSF53623   MurD-like peptide ligases, catalytic domain
 [115-313]  1.7e-51 PF08245
PF08245   Mur_ligase_M
 [89-330]  6e-57 G3DSA:3.40.1190.10
G3DSA:3.40.1190.10   no description
SignalP No significant hit
TMHMM No significant hit
SOSUI No significant hit

close this sectionCalculated information (Amino acid sequence)

size of protein 492 amino acids
molecular mass 51,476.80 Da
pI 5.2
aa composition
AlaValLeuIlePheTrpProMetGlySer
14.4
(71aa)		11.4
(56aa)		7.7
(38aa)		2.8
(14aa)		2.2
(11aa)		1
(5aa)		5.3
(26aa)		1.8
(9aa)		10.2
(50aa)		4.9
(24aa)
ThrCysAsnGlnTyrLysHisArgAspGlu
7.1
(35aa)		0.8
(4aa)		2.8
(14aa)		1.8
(9aa)		1
(5aa)		1.4
(7aa)		3.7
(18aa)		6.9
(34aa)		6.1
(30aa)		6.5
(32aa)

open this sectionSequence

close this sectionCovered clones

NBRC No. clone name start stop length(bp)
G09-002-042 MDFO1S8503E21 1,649,995 1,692,514 42,520
G09-002-043 MDFO1S8502I17 1,671,320 1,711,203 39,884

close this sectionDistribution of Our Microbial Genomic DNA clones

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.