CDS Information : KRH_21260

Display	1000bp 10000bp 100000bp Clone

Kocuria rhizophila DC2201 (= NBRC 103217) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Kocuria rhizophila DC2201 (= NBRC 103217)
Replicon	chromosome
Start / Stop / Direction	2,445,339 / 2,446,349 / +
Location	2445339..2446349
Type	CDS
Length	1,011 bp (336 aa)

Product	branched-chain alpha-keto acid dehydrogenase E1 beta subunit
Gene name
Functional category	Metabolism - Amino Acid Metabolism
EC number	1.2.4.4
Note	Similar to the Rv2496c of Mycobacterium tuberculosis (O06160; PMID:16045627).
KEGG pathway	map:00280 ec:1.2.4.4
Sequence feature
References	Uniprot:Q53593 Pubmed:8002616

UniProt	B2GHW1
INSD	BAG30473.1 (DDBJ GenBank EMBL)
UniParc	UPI0001734344

BLASTP Database:UniProtKB:12.5 show BLAST table	1 E=1e-140 A1R9E3_ARTAT Putative pyruvate dehydrogenase E1 component, beta subunit Alignment 2 E=1e-138 A0JZV0_ARTS2 Transketolase, central region Alignment 3 E=1e-129 A4X6P9_9ACTO Transketolase, central region Alignment 4 E=1e-127 A1GFJ7_9ACTO Transketolase, central region Alignment 5 E=1e-125 Q0SBG3_RHOSR Pyruvate dehydrogenase E1 component beta subunit Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR005475 Transketolase, central region (Domain) PF02779   Transket_pyr IPR005476 Transketolase, C-terminal (Domain) PF02780   Transketolase_C IPR009014 Transketolase, C-terminal-like (Domain) G3DSA:3.40.50.920   no description SSF52922   TK C-terminal domain-like
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	336 amino acids
molecular mass	36,289.83 Da
pI	4.7
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 12.2 (41aa) 10.1 (34aa) 6.3 (21aa) 3.6 (12aa) 4.5 (15aa) 0.6 (2aa) 5.7 (19aa) 3 (10aa) 7.7 (26aa) 8.3 (28aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 4.8 (16aa) 1.2 (4aa) 1.5 (5aa) 2.1 (7aa) 3 (10aa) 2.4 (8aa) 3 (10aa) 6.3 (21aa) 6 (20aa) 8 (27aa)

>KRH_21260 branched-chain alpha-keto acid dehydrogenase E1 beta subunit
ATGAGCGCTTCGAAAACCAGTTTCGCCAAGGCGCTGAACACCGCGCTCGCGGACGCCATG
GAGGCATCCCGGGAGGTCGTGGTCTTCGGTGAGGACGTGGGCACCCTCGGCGGCGTCTTC
CGCATCACCGACGGGCTGACCGCGCGCTTCGGCCGCGAGCGCTGCTTCGACACCCCGCTC
GCGGAGTCCGGGATCATCGGCACCGCGGTGGGCATGGCCATGAACGGCATGCGCCCCGTG
GCGGAGATGCAGTTCGACGCGTTCGCGTACCCCGCGTTCGAGCAGGTGGCCTCGCACGTG
GCCAAGATGCACAACCGCACCCGGGGCAAGCTGAGCATGCCCCTGGTCATCCGCATCCCC
TACGGCGGCGGCGTGGGCGGCGTGGAGCACCACTGCGACTCCTCCGAGTCCTACTACGCG
CACACCCCCGGGCTGAAGGTCTTCACCCCGGCCTCGGTGACGGACGCGTACGTGATGCTG
CGCGAGGCCATCGACTCCCCGGACCCTGTGGTGTTCTTCGAGCCCAAGCGGCTGTACTGG
TCCACCGCGCAGGTGGACCTGGAGGAGCTGCGCGAGCAGTACGAGAACGGGAACCTGCCC
AAGCGCGAGGGCCACGCCGTCGTCGCCCGTGAGGGCAGTGACGCAACCCTGATCTCCTAC
GGCCCGTCCGTGCCGGTGTGCCTGGCCGCGGCCGAGGAGGCCGCGAAGGACGGCATGTCC
GTGGAGGTCATCGACCTCGGCTCCGTGGTGCCCTACGACGACGAGTCCGTGGCCGCCTCC
GTGCGCCGCACCGGGCGCGCCGTGGTGGTGGCCGAGTCCCAGGGCTTCGCGTCCGTGGCC
TCGGAGATCGCCGCGCGCACCCAGGAGCGCTGCTTCCACTCGCTCGCCGCGCCGGTGCTG
CGGGTCACGGGATTCGACATCCCCTACCCCTCACCGTCCTTCGAGCACCACCACATCCCG
AGTTCCGAGCGCATCCTGGATGCCCTGGACGACCTGCAGTGGGAGGACTGA


>KRH_21260 branched-chain alpha-keto acid dehydrogenase E1 beta subunit
MSASKTSFAKALNTALADAMEASREVVVFGEDVGTLGGVFRITDGLTARFGRERCFDTPL
AESGIIGTAVGMAMNGMRPVAEMQFDAFAYPAFEQVASHVAKMHNRTRGKLSMPLVIRIP
YGGGVGGVEHHCDSSESYYAHTPGLKVFTPASVTDAYVMLREAIDSPDPVVFFEPKRLYW
STAQVDLEELREQYENGNLPKREGHAVVAREGSDATLISYGPSVPVCLAAAEEAAKDGMS
VEVIDLGSVVPYDDESVAASVRRTGRAVVVAESQGFASVASEIAARTQERCFHSLAAPVL
RVTGFDIPYPSPSFEHHHIPSSERILDALDDLQWED

NBRC No.	clone name	start	stop	length(bp)
G09-002-065	MDFO1S8503I21	2,431,515	2,469,002	37,488
G09-002-066	MDFO1S8502B06	2,433,229	2,477,869	44,641

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.