CDS Information : KRH_22010

Display	1000bp 10000bp 100000bp Clone

Kocuria rhizophila DC2201 (= NBRC 103217) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Kocuria rhizophila DC2201 (= NBRC 103217)
Replicon	chromosome
Start / Stop / Direction	2,529,586 / 2,528,822 / -
Location	complement(2528822..2529586)
Type	CDS
Length	765 bp (254 aa)

Product	2-oxo-hepta-3-ene-1,7-dioic acid hydratase
Gene name	hpaH
Functional category	Metabolism - Biosynthesis of Secondary Metabolites and Biodegradation of Xenobiotics
EC number	4.2.-.-
Note
KEGG pathway	map:00350 KO:K02509
Sequence feature
References	Uniprot:Q46982 Pubmed:8550403 Uniprot:P42270 Pubmed:7737515

UniProt	B2GIC6
INSD	BAG30548.1 (DDBJ GenBank EMBL)
UniParc	UPI000173443B

BLASTP Database:UniProtKB:12.5 show BLAST table	1 E=1e-111 Q5YW19_NOCFA Putative hydratase Alignment 2 E=1e-103 A0K0S9_ARTS2 2-oxo-hepta-3-ene-1,7-dioic acid hydratase Alignment 3 E=1e-101 A1RBH7_ARTAT 2-oxo-hepta-3-ene-1,7-dioic acid hydratase Alignment 4 E=1e-98 A1RDE3_ARTAT Putative 2-oxo-hepta-3-ene-1,7-dioic acid hydratase Alignment 5 E=3e-83 Q0RKA5_FRAAA 2-oxo-hepta-3-ene-1,7-dioic acid hydratase OHED hydratase Alignment
HAMAP	No significant hit
InterPro Database:interpro:16.0	IPR002607 Hydratase/decarboxylase (Family) PD003721   Q8YDN7_BRUME_Q8YDN7; PF01689   Hyratase_decarb IPR011234 Fumarylacetoacetase, C-terminal-related (Domain) SSF56529   FAH G3DSA:3.90.850.10   no description
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	254 amino acids
molecular mass	27,703.25 Da
pI	4.65
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 13 (33aa) 9.4 (24aa) 8.7 (22aa) 5.1 (13aa) 2 (5aa) 1.6 (4aa) 3.5 (9aa) 2.8 (7aa) 8.3 (21aa) 4.3 (11aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 5.9 (15aa) 0.4 (1aa) 3.9 (10aa) 2.4 (6aa) 3.1 (8aa) 1.6 (4aa) 2.8 (7aa) 7.1 (18aa) 5.5 (14aa) 8.7 (22aa)

>KRH_22010 2-oxo-hepta-3-ene-1,7-dioic acid hydratase
GTGGCCATCGCGGACGAACTGGCCGCCGCGGAGGCGAACCGCACGATGATCGAGCGGCTC
ACGCTGCGCTACCCGGAGATGACCGTGGAGGACTCCTACGCGGTGCAGAACGAGTGGCGC
CGGCGCGCGCAGGAGGCCGGGCGGCGGCTCGTGGGCCGGAAGATCGGGCTGACCTCGAAG
GTCATGCAGGAGGCCACGGGGATCACGGAACCGGACTACGGGGCGATCTTCGCGGACCAG
GTGCACGAGAACGGCTCGGTGATCGAGCACGGGCAGTACTCGAACGTGCGGATCGAGGTG
GAGCTCGCGTTCGTGCTCAGGGACGAGCTGCGCGGGCCGCACACGAGCATCTTCGACGTG
CTGCGGGCCACCGAGTACGTGGTGCCGGCGCTGGAGATCCTCAGCTCGAGGGTCCAGATG
GAGGGCCGCACGATCGTGGACACCATCTCGGACAATGCCGCCCTCGGCGGGATGGTCTAC
GGCGGCAACCCCGTGGCTCCGGACGCCGTGGACCTGCGGTGGGTCTCCGCGCTGCTGTAC
CGCAACGAGACCCTGGAGGACACGGGCGTGGCCGCGGCCGTGCTCAACCACCCCGCGAAC
GGCGTGGCGTGGCTCGCCAACAAGCTGGCCGCGCACGGGGACGCGCTGGAGGCCGGGAGC
ATCGTGCTGGCCGGGTCCTTCACCAAGCCTATGTGGGTGCACCCTGGGGACACCGTCCAC
GCGGACTACGGAGAATTGGGAGCCATCACGTGTCGCTTCGAGTGA


>KRH_22010 2-oxo-hepta-3-ene-1,7-dioic acid hydratase
MAIADELAAAEANRTMIERLTLRYPEMTVEDSYAVQNEWRRRAQEAGRRLVGRKIGLTSK
VMQEATGITEPDYGAIFADQVHENGSVIEHGQYSNVRIEVELAFVLRDELRGPHTSIFDV
LRATEYVVPALEILSSRVQMEGRTIVDTISDNAALGGMVYGGNPVAPDAVDLRWVSALLY
RNETLEDTGVAAAVLNHPANGVAWLANKLAAHGDALEAGSIVLAGSFTKPMWVHPGDTVH
ADYGELGAITCRFE

NBRC No.	clone name	start	stop	length(bp)
G09-002-068	MDFO1S8502D08	2,499,178	2,533,485	34,308
G09-002-069	MDFO1S8503K17	2,521,232	2,560,713	39,482

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.