CDS Information : KSE_22660

Display	1000bp 10000bp 100000bp Clone

Kitasatospora setae NBRC 14216T chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Kitasatospora setae NBRC 14216T
Replicon	chromosome
Start / Stop / Direction	2,509,043 / 2,509,903 / +
Location	2509043..2509903
Type	CDS
Length	861 bp (286 aa)

Product	putative phosphoenolpyruvate mutase
Gene name
Functional category	- metabolism of carbohydrates and related molecules
EC number	5.4.2.9
Note	Also similar to Phosphonopyruvate hydrolase in Variovorax sp. Pal2 (Q84G06).
KEGG pathway
Sequence feature	56 (a.a.) ACT_SITE
References	Uniprot:Q84G06 Pubmed:12697754 Uniprot:P56839 Pubmed:10571990,10378273

BLASTP Database:UniProtKB:2010_04 show BLAST table	1 E=7e-96 Q0RC29_FRAAA Putative Phosphoenolpyruvate phosphomutase (Phosphoenolpyruvate mutase) (PEP mutase) (PEP phosphomutase) Alignment 2 E=9e-95 C4RIA5_9ACTO Phosphoenolpyruvate phosphomutase Alignment 3 E=2e-70 A9A1T5_NITMS Putative phosphoenolpyruvate phosphomutase Alignment 4 E=2e-64 B3T5P0_9ZZZZ Putative uncharacterized protein Alignment 5 E=1e-61 A8TJJ4_9PROT Phosphoenolpyruvate phosphomutase Alignment
HAMAP	No significant hit
InterPro Database:interpro:26.0	IPR000918 Isocitrate lyase/phosphorylmutase (Domain) PF00463   ICL IPR000918 Isocitrate lyase and phosphorylmutase (Family) PF00463   ICL IPR015813 Pyruvate/Phosphoenolpyruvate kinase, catalytic core (Domain) G3DSA:3.20.20.60   no description SSF51621   Phosphoenolpyruvate/pyruvate domain
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	286 amino acids
molecular mass	30,722.62 Da
pI	4.87
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 14 (40aa) 10.1 (29aa) 8 (23aa) 4.9 (14aa) 3.8 (11aa) 1.4 (4aa) 2.1 (6aa) 1.7 (5aa) 7.3 (21aa) 8.4 (24aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 4.5 (13aa) 1.4 (4aa) 2.4 (7aa) 4.2 (12aa) 2.4 (7aa) 2.8 (8aa) 2.8 (8aa) 5.2 (15aa) 7 (20aa) 5.2 (15aa)

>KSE_22660 putative phosphoenolpyruvate mutase
GTGTTGCGTGTCAACCACCAGTCCTTCCGCACCGTTCTCGCCGAGTCGGACGTCGTCCGC
GTCGCCGGGGCGCACCACGCGCTCAGCGGCTACCTGGCCCAGGAGGCGGGCTTCCAGGCC
GTCTGGTCGTCCAGCTTCGAGGTGTCGGCCTCCCGCTGCCTGCCCGACGCCAGCATCCTG
AGCATGACCGAGTACCTGGAGGCGGCGGCCAACATCCAGAAGGCGCTGAGCATCCCGGTG
GTCGCCGACTGCGACACCGGCTACGGCGACAACCTCAACGTGGCGCACATGGTCCACGAG
TTCGAGGGCGCCGGGATCACCGCGGTCTGCATCGAGGACAAGCTGTTCCCGAAGATGAAC
AGCTTCGTCGGCAGCGGCCAGGCCCTGCTGGACACCGCCGGCTTCGCCGCCAAGATCCGC
ACCGCCAAGAACGCCCAGCACAGCTCCGACTTCTTCGTCATCGCCCGCACCGAGGCGCTG
ATCAGCGGCCTCGACGTCACCCAGGCGCTGGAGCGCTGCCGCGCCTACGCCGACTCCGGC
GCGGACGCCGTGCTGGTGCACTCCAAGCAGCGCACCAAGGACCAGGTGATGGCCTTCCTG
GACGGCTGGGACGGCCGCGTCCCGGTGGTCATCGTGCCCACCACCTACCCCGACTGGCAC
ATCGACGAGGTGCGGGCGGCCGGGGTCTCCGTGGTGATCTACGCCAACCAGGGCCTGCGC
GCCAGCGTCTCCTCGCTGCGCGACGCCTACGGCGCGATCGTCCGGGACGGCGACACCACC
GGCATCGAGGAGTCCATCGCCTCGGTCGGCGACCTGTTCGAGCTCCAGCGGCTCGCCGAG
TGGCAGAAGCTCGGTGCCTGA


>KSE_22660 putative phosphoenolpyruvate mutase
MLRVNHQSFRTVLAESDVVRVAGAHHALSGYLAQEAGFQAVWSSSFEVSASRCLPDASIL
SMTEYLEAAANIQKALSIPVVADCDTGYGDNLNVAHMVHEFEGAGITAVCIEDKLFPKMN
SFVGSGQALLDTAGFAAKIRTAKNAQHSSDFFVIARTEALISGLDVTQALERCRAYADSG
ADAVLVHSKQRTKDQVMAFLDGWDGRVPVVIVPTTYPDWHIDEVRAAGVSVVIYANQGLR
ASVSSLRDAYGAIVRDGDTTGIEESIASVGDLFELQRLAEWQKLGA

NBRC No.	clone name	start	stop	length(bp)
G20-001-099	KS1E11L00007E05	2,484,054	2,522,325	38,272

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.