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CDS Information : KSE_23300

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close this sectionGenomic map

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Kitasatospora setae NBRC 14216T chromosome

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Overview 
Genome

close this sectionLocation

Organism Kitasatospora setae NBRC 14216T
Replicon chromosome
Start / Stop / Direction 2,585,663 / 2,583,525 / -
Location complement(2583525..2585663)
Type CDS
Length 2,139 bp (712 aa)

close this sectionAnnotation

Product putative alpha-amylase
Synonym: 1,4-alpha-D-glucan glucanohydrolase
Gene name
Functional category
- metabolism of carbohydrates and related molecules
- - specific pathways
EC number 3.2.1.1
Note
KEGG pathway map:00500 KO:K01176
Sequence feature

130 (a.a.)METAL
176 (a.a.)METAL
185 (a.a.)METAL
215 (a.a.)ACT_SITE
219 (a.a.)METAL
242 (a.a.)ACT_SITE
304 (a.a.)ACT_SITE


References Family/Domain:IPR006046
Uniprot:P09794 Pubmed:3500166

close this sectionComputational search results

BLASTP
Database:UniProtKB:2010_04
1E=0A4X6F5_SALTOAlpha-amylaseAlignment
2E=1e-173D2BDI7_STRRDGlycosidase-like proteinAlignment
3E=1e-170C6WQ29_ACTMDAlpha amylase catalytic regionAlignment
4E=1e-168Q9L035_STRCOSecreted alpha-amylaseAlignment
5E=1e-168D1XEJ5_9ACTOAlpha amylase catalytic regionAlignment
HAMAP No significant hit
InterPro
Database:interpro:26.0
IPR002044 Glycoside hydrolase, carbohydrate-binding (Domain)
 [611-712]  22.563 PS51166
PS51166   CBM20
 [619-704]  1.2e-29 PF00686
PF00686   CBM_20
IPR005085 Carbohydrate binding family 25 (Domain)
 [500-576]  5.3e-17 PF03423
PF03423   CBM_25
IPR006046 Glycoside hydrolase family 13 (Domain)
 [88-105]  1.6e-22 PR00110 [120-131]  1.6e-22 PR00110 [209-220]  1.6e-22 PR00110 [238-256]  1.6e-22 PR00110 [294-306]  1.6e-22 PR00110
PR00110   ALPHAAMYLASE
IPR006046 Glycoside hydrolase family 13 (Family)
 [88-105]  1.6e-22 PR00110 [120-131]  1.6e-22 PR00110 [209-220]  1.6e-22 PR00110 [238-256]  1.6e-22 PR00110 [294-306]  1.6e-22 PR00110
PR00110   ALPHAAMYLASE
IPR006047 Glycosyl hydrolase, family 13, catalytic region (Domain)
 [91-340]  2.2e-27 PF00128
PF00128   Alpha-amylase
IPR006048 Alpha-amylase, C-terminal all beta (Domain)
 [389-475]  1.3e-38 SM00632
SM00632   no description
 [392-472]  4.2e-10 PF02806
PF02806   Alpha-amylase_C
IPR006311 Twin-arginine translocation pathway, signal sequence (Conserved_site)
 [1-38]  8.676 PS51318
PS51318   TAT
IPR006589 Glycosyl hydrolase, family 13, subfamily, catalytic region (Domain)
 [45-380]  7.8e-79 SM00642
SM00642   no description
IPR013780 Glycosyl hydrolase, family 13, all-beta (Domain)
 [386-476]  2.5e-25 G3DSA:2.60.40.1180
G3DSA:2.60.40.1180   no description
IPR013781 Glycoside hydrolase, catalytic core (Domain)
 [35-385]  1.2e-105 G3DSA:3.20.20.80
G3DSA:3.20.20.80   no description
IPR013783 Immunoglobulin-like fold (Domain)
 [611-712]  1.4e-29 G3DSA:2.60.40.10
G3DSA:2.60.40.10   no description
IPR013784 Carbohydrate-binding-like fold (Domain)
 [610-712]  1.1e-31 SSF49452
SSF49452   Starch-binding domain-like
IPR017853 Glycoside hydrolase, catalytic core (Domain)
 [35-384]  2.8e-85 SSF51445
SSF51445   (Trans)glycosidases
IPR017909 Twin arginine translocation signal, Tat (Domain)
 [1-38]  8.676 PS51318
PS51318   TAT
SignalP
 [1-38]  1 Signal
Bacteria, Gram-positive   
TMHMM
 [12-34]  Transmembrane (i-o)
Transmembrane 1   
SOSUI No significant hit

close this sectionCalculated information (Amino acid sequence)

size of protein 712 amino acids
molecular mass 73,132.36 Da
pI 6.24
aa composition
AlaValLeuIlePheTrpProMetGlySer
14.3
(102aa)
7.9
(56aa)
5.1
(36aa)
2
(14aa)
2.7
(19aa)
2.7
(19aa)
4.4
(31aa)
0.8
(6aa)
13.3
(95aa)
8
(57aa)
ThrCysAsnGlnTyrLysHisArgAspGlu
9.7
(69aa)
1.4
(10aa)
6.2
(44aa)
2.7
(19aa)
4.5
(32aa)
2.9
(21aa)
1.3
(9aa)
3.2
(23aa)
5.2
(37aa)
1.8
(13aa)

open this sectionSequence