CDS Information : RER_09640

Display	1000bp 10000bp 100000bp Clone

Rhodococcus erythropolis PR4 (= NBRC 100887) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Rhodococcus erythropolis PR4 (= NBRC 100887)
Replicon	chromosome
Start / Stop / Direction	1,059,791 / 1,061,038 / +
Location	1059791..1061038
Type	CDS
Length	1,248 bp (415 aa)

Product	putative Mce family protein
Gene name	mce3E
Functional category	Unclassified - uncharacterized protein
EC number
Note	The genome of PR4 is predicted to have five copies of the mce operon, and this gene is a component of mce3 operon. Similar to the possible mce-family lipoprotein LprN (Mce4E, Rv3495c) of Mycobacterium tuberculosis (O53540; PMID:12052567, 14500535).
KEGG pathway
Sequence feature
References

BLASTP Database:UniProtKB:14.0 show BLAST table	1 E=1e-145 Q0S7J8_RHOSR MCE family protein Alignment 2 E=1e-128 Q5Z2F5_NOCFA Putative Mce family protein Alignment 3 E=4e-93 A4T966_MYCGI Virulence factor Mce family protein precursor Alignment 4 E=6e-92 A0R1K5_MYCS2 Virulence factor mce family protein Alignment 5 E=1e-91 A0PUH3_MYCUA MCE-family lipoprotein LprM_1 Alignment
HAMAP	No significant hit
InterPro Database:interpro:18.0	IPR003399 Mammalian cell entry related (Domain) PF02470   MCE IPR005693 Mammalian cell entry (Family) TIGR00996   Mtu_fam_mce: virulence factor Mce family pr
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	415 amino acids
molecular mass	42,581.10 Da
pI	4.22
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 12 (50aa) 7.7 (32aa) 12.3 (51aa) 4.8 (20aa) 1 (4aa) 0.5 (2aa) 8.7 (36aa) 1.2 (5aa) 10.4 (43aa) 6.3 (26aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 6.5 (27aa) 0.2 (1aa) 4.8 (20aa) 6.5 (27aa) 0.7 (3aa) 1.9 (8aa) 0.7 (3aa) 3.6 (15aa) 5.1 (21aa) 5.1 (21aa)

>RER_09640 putative Mce family protein
ATGCGGGTAGCTCGCAAGACCACGGTTGCCGTGTTGGCCACGGCAGTGGCACTCGGCGCC
AGCGGTTGTGAATGGAACGGTCTCAACTCTGTGCCGATGCCAGGAACTCAGGGCCGGGGC
GAAGGCGCATACGAGGTCCAGATCGAGATGCCGAACGTGACTACCCTGTCGCAGAATTCG
CCCGTCCGGGTGGATGACGTTGCAGTGGGAAGCGTTTCGAACATCGAGGTTCAGGGCTGG
CACGCTCTGGTGACGGTGTCGCTGAATCCCGAGGTTCAGTTGCCTGCCAACGCGACGGCA
AAAATCGGCCAGACCAGCTTGCTTGGCTCTCAGCACATCGAGTTGGCACCGCCGGTTGGA
GAAGAGCCCGAGGGCCGACTCGAAGCCGGTGACTTGATTCCCATCGAGCGGGCGGGCGCT
TACCCGACGACGGAACAGACGTTGTCGTCGCTGTCGGTTGTTCTCAACGGCGGCGGTCTC
GCTCAGGTTCAGGACATCACGAAGGAACTCAACGCCGCTCTCGGTGGGCGTGAAGATTCC
GTTCGAGACCTCCTACCTCAGCTCGATCAGCTGGTCGGAAGTCTGGACAAGCAACGAGGC
GACATCGTTTCGGCGATGGAAGGACTCGATCGACTGTCCTCGACCGTCCAGGCACAGCAC
GACACCTTGAACAAAGCGCTCGAAGGTATTCCGCCGGCACTCGAGATTCTTGTCGCTCAG
CGTCAGGACATCACCAACGCGTTGACCTCCCTCGGTGGTCTCAGCGAGGTGGCGAGCCGT
GTCATCCAGGAGAGCGGCGACGATTTCGCGACCAATGTTCGGGCTCTTTCGCCGACTCTG
AAGGCCTTGGCGGATTCGGGTAACGCTCTCACTCAGGTTCTCGGAGTCCTCTTGACGTTC
CCGTTCCCACAGAACGGAATCGACAACGTCATCCGCGGTGACTACGCCAACCTGGCGATG
ACCATCGACATCTCGCTGCCCCGACTCGACGCGAACTTCCTGACGGGAACTCCGTTGGCC
GGAACGCTCTCCGGACCAGAAGGTGTGTTGGGTAAGCAGGCGGGGCTCGCCGGACAGACA
GGCAACCCGTTGAGCGACCCGCTCAAGCCTGCCGCACCGCCGGCACAAGCTCCCGCCGGA
CAGGCTCCCGCTGCGCAGGCCCCGGCAGCGGATCCGAATACCATTCAGATTCCGGGACTT
CCGCCGATCAACATTCCAGGACTTCCTCAGGGGGCACCGGCACCATGA


>RER_09640 putative Mce family protein
MRVARKTTVAVLATAVALGASGCEWNGLNSVPMPGTQGRGEGAYEVQIEMPNVTTLSQNS
PVRVDDVAVGSVSNIEVQGWHALVTVSLNPEVQLPANATAKIGQTSLLGSQHIELAPPVG
EEPEGRLEAGDLIPIERAGAYPTTEQTLSSLSVVLNGGGLAQVQDITKELNAALGGREDS
VRDLLPQLDQLVGSLDKQRGDIVSAMEGLDRLSSTVQAQHDTLNKALEGIPPALEILVAQ
RQDITNALTSLGGLSEVASRVIQESGDDFATNVRALSPTLKALADSGNALTQVLGVLLTF
PFPQNGIDNVIRGDYANLAMTIDISLPRLDANFLTGTPLAGTLSGPEGVLGKQAGLAGQT
GNPLSDPLKPAAPPAQAPAGQAPAAQAPAADPNTIQIPGLPPINIPGLPQGAPAP

NBRC No.	clone name	start	stop	length(bp)
G12-001-042	REGR1S7013A02	1,033,679	1,075,766	42,088

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.