CDS Information : RER_23970

Display	1000bp 10000bp 100000bp Clone

Rhodococcus erythropolis PR4 (= NBRC 100887) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Rhodococcus erythropolis PR4 (= NBRC 100887)
Replicon	chromosome
Start / Stop / Direction	2,591,038 / 2,592,978 / +
Location	2591038..2592978
Type	CDS
Length	1,941 bp (646 aa)

Product	acetolactate synthase large subunit Synonym: acetohydroxyacid synthase
Gene name	ilvB
Functional category	Metabolism - Amino Acid Metabolism
EC number	2.2.1.6
Note	May form a complex with the small subunit (encoded by RER_23980).
KEGG pathway	map:00290 KO:K01652 map:00770 KO:K01652
Sequence feature
References	Uniprot:P42463 Pubmed:8366043

BLASTP Database:UniProtKB:14.0 show BLAST table	1 E=0 Q0S2H5_RHOSR Acetolactate synthase large subunit Alignment 2 E=0 Q5YRW0_NOCFA Acetolactate synthase Alignment 3 E=0 B1MDT0_MYCAB Acetolactate synthase Alignment 4 E=0 A3PXP7_MYCSJ Acetolactate synthase, large subunit Alignment 5 E=0 A1UE93_MYCSK Acetolactate synthase, large subunit Alignment
HAMAP	No significant hit
InterPro Database:interpro:18.0	IPR000399 TPP-binding enzymes, conserved site (Conserved_site) PS00187   TPP_ENZYMES IPR000399 TPP-binding enzymes (Family) PS00187   TPP_ENZYMES IPR011766 Thiamine pyrophosphate enzyme, C-terminal TPP-binding (Domain) PF02775   TPP_enzyme_C IPR012000 Thiamine pyrophosphate enzyme, central region (Domain) PF00205   TPP_enzyme_M IPR012001 Thiamine pyrophosphate enzyme, N-terminal TPP binding region (Domain) PF02776   TPP_enzyme_N IPR012846 Acetolactate synthase, large subunit, biosynthetic (Family) TIGR00118   acolac_lg: acetolactate synthase, large sub PTHR18968:SF13   ACETOLACTATE SYNTHASE
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	646 amino acids
molecular mass	68,984.41 Da
pI	4.7
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 12.5 (81aa) 8 (52aa) 6.8 (44aa) 6.2 (40aa) 2.5 (16aa) 1.2 (8aa) 7 (45aa) 3.1 (20aa) 9.1 (59aa) 4.2 (27aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 7.1 (46aa) 1.1 (7aa) 2.5 (16aa) 4.2 (27aa) 2.3 (15aa) 2.9 (19aa) 1.9 (12aa) 5.3 (34aa) 6.3 (41aa) 5.7 (37aa)

>RER_23970 acetolactate synthase large subunit
GTGAGCGCACCAACAGCACGGCCTCAACCCACGCCGCGCAAGTCAGGGACACCGAGCCCG
ACAACCGCGGCTGCCGCAACCCAGACGGCCAACCGTCGCCAGCTCCCGCCTGAGCGGGTA
ACCGGCGCGCAGTCTGTGGTTCGAGCCCTCGAAGAGCTCGAGGTCGACACGGTATTCGGC
ATTCCCGGCGGTGCCGTGCTCCCGGTTTACGACCCACTCTTCGATTCGGTCAAGGTCCGC
CACGTGCTCGTGCGCCACGAGCAGGGTGCAGGCCACGCTGCCACCGGTTACGCCCAGGCG
ACCGGCAAGGTCGGCGTCTGCATGGCTACCTCGGGTCCCGGTGCGACCAACCTGGTCACG
CCACTGGCCGACGCGCAGATGGACTCCGTTCCGATCGTCGCGATCACCGGACAGGTGGCG
CGCTCGCTGATCGGTACCGACGGCTTCCAGGAAGCCGACATCTCAGGCATCACGATGCCG
GTCACCAAGCACAACTTCCTGATCACCGACGCGATCGACATCCCGCGCATCATGGCCGAA
GCCTTCTACCTCGCGGCCAGCGGTCGCCCCGGCGCCGTCCTGGTCGATATCCCCAAGGAC
ATCCTGCAGTCGCAGACCACGTTCTCGTGGCCGCCGGAGATGCGTCTGCCGGGCTACCGC
CCGGTCACCAAGCCGCACGGCAAGCAGGTTCGTGAAGCTGCACGTCTGATCGCCGACGCC
AAGTCGCCGGTTCTGTACGTCGGTGGCGGCGTCATCAAGTCGGACTCCTCGCCGGAGCTG
CTCGAATTGGCAGAGCTGACCGGTATCCCCGTCGTCACCACGCTGATGGCCCGCGGCGCG
TTCCCGGACAGCCACGACCTCAACTGCGGTATGCCGGGAATGCACGGCACCGTTGCCGCA
GTCGCCGCTCTGCAGCGCAGCGATCTGCTGATCACCCTCGGCGCGCGTTTCGACGACCGC
GTCACCGGTCAGCTGAATTCCTTCGCGCCCGATGCCAAGGTCATCCACGCCGACATCGAT
CCGGCCGAGATCGGCAAGAACCGCTACGCCGACGTCCCGATCGTGGGCGACTGCAAAGAG
GTCATCACCGAACTCATCGAGGCTATCCGTGCCGACATGGCCACGGGCACCACGTTCGAA
CTCTCGGAATGGTGGGCGTACCTGAACGACATCCGTCGTACCTACCCGCTGAGCTACGAC
CGTCCCACAGACGGTCAGCTGAGCCCGGAGTACGTCATCCAGACCGTCGGCAAGCTCGCC
GGACCGGATGCCATCTACTGCGCCGGCGTCGGCCAGCACCAGATGTGGGCCGCGCAGTTC
GTCGGTTACGAGAAGCCGCGCACGTGGCTCAACTCGGGCGGGCTCGGCACCATGGGGTAC
GCGGTCCCCGCAGCCATGGGCGCCAAGATGGGCATGCCCGACACCGAGGTGTGGGCGATC
GACGGCGACGGCTGCTTCCAGATGACCAATCAGGAACTCGCAACCTGCGCACTCGAAGGT
ATCCCGATCAAGGTTGCCCTCATCAACAACGGCAACCTCGGCATGGTGCGTCAGTGGCAG
ACACTCTTCTACGAAGAGCGCTACTCCAACACCAACCTCGGAACACACGGCGCCATCCGC
ATCCCGGACTTCGTCAAGCTCGCAGAAGCATTGGGCTGCCACGGAATTCGTGTCGAGCGC
GAAGAAGATGTCGAAGCTGCGATCCGTGAGGCGCAGTCCATCAACGACAAGCCTGTCGTG
ATCGACTTCATCGTCACCGCCGACGCACAGGTGTGGCCGATGGTCGCTGCCGGAACGAGC
AACGACGAAATCATGGCGGCCCGGGGCATCCGGCCGCTGTTCGACGACGACGAGGCAGTT
GCCGAGCCCGCCGTCATCCACGAAGCCATGTCACGCGAGCAAGCCGGACCCGCCGCGGGC
ACAGGGGAGGATCAGAAGTGA


>RER_23970 acetolactate synthase large subunit
MSAPTARPQPTPRKSGTPSPTTAAAATQTANRRQLPPERVTGAQSVVRALEELEVDTVFG
IPGGAVLPVYDPLFDSVKVRHVLVRHEQGAGHAATGYAQATGKVGVCMATSGPGATNLVT
PLADAQMDSVPIVAITGQVARSLIGTDGFQEADISGITMPVTKHNFLITDAIDIPRIMAE
AFYLAASGRPGAVLVDIPKDILQSQTTFSWPPEMRLPGYRPVTKPHGKQVREAARLIADA
KSPVLYVGGGVIKSDSSPELLELAELTGIPVVTTLMARGAFPDSHDLNCGMPGMHGTVAA
VAALQRSDLLITLGARFDDRVTGQLNSFAPDAKVIHADIDPAEIGKNRYADVPIVGDCKE
VITELIEAIRADMATGTTFELSEWWAYLNDIRRTYPLSYDRPTDGQLSPEYVIQTVGKLA
GPDAIYCAGVGQHQMWAAQFVGYEKPRTWLNSGGLGTMGYAVPAAMGAKMGMPDTEVWAI
DGDGCFQMTNQELATCALEGIPIKVALINNGNLGMVRQWQTLFYEERYSNTNLGTHGAIR
IPDFVKLAEALGCHGIRVEREEDVEAAIREAQSINDKPVVIDFIVTADAQVWPMVAAGTS
NDEIMAARGIRPLFDDDEAVAEPAVIHEAMSREQAGPAAGTGEDQK

NBRC No.	clone name	start	stop	length(bp)
G12-001-101	REGR1S7017E07	2,560,218	2,602,480	42,263
G12-001-102	REGR1S8010A07	2,589,809	2,626,057	36,249

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.