CDS Information : RER_24030

Display	1000bp 10000bp 100000bp Clone

Rhodococcus erythropolis PR4 (= NBRC 100887) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Rhodococcus erythropolis PR4 (= NBRC 100887)
Replicon	chromosome
Start / Stop / Direction	2,598,713 / 2,599,873 / +
Location	2598713..2599873
Type	CDS
Length	1,161 bp (386 aa)

Product	alkane-1-monooxygenase
Gene name	alkB
Functional category	Metabolism - Biosynthesis of Secondary Metabolites and Biodegradation of Xenobiotics
EC number	1.14.15.3
Note	May be involved in alkane degradation. Identical to the alkane-1-monooxygenase (AlkB4) of Rhodococcus erythropolis NRRL B-16531 (Q93JS1). Identical to the alkane-1-monooxygenase (AlkB4) of Rhodococcus sp. Q15 (Q93DN4).
KEGG pathway	map:00071 KO:K00496
Sequence feature
References	Uniprot:Q93DN4 Pubmed:12450813

BLASTP Database:UniProtKB:14.0 show BLAST table	1 E=0 Q93JS1_RHOER Putative alkane-1-monooxygenase 4 Alignment 2 E=0 Q93DN4_RHOSQ Putative alkane-1-monooxygenase Alignment 3 E=1e-166 Q93DN5_RHOSQ Alkane-1-monooxygenase Alignment 4 E=1e-165 Q2XUJ8_9NOCA Alkane monooxygenase AlkB3 Alignment 5 E=1e-165 Q93JS2_RHOER Putative alkane-1-monooxygenase 3 Alignment
HAMAP	No significant hit
InterPro Database:interpro:18.0	IPR005804 Fatty acid desaturase, type 1 (Domain) PF00487   FA_desaturase
SignalP	No significant hit
TMHMM	Transmembrane 5
SOSUI	Transmembrane 5

size of protein	386 amino acids
molecular mass	43,871.57 Da
pI	9.45
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 11.9 (46aa) 7.3 (28aa) 11.1 (43aa) 3.9 (15aa) 4.9 (19aa) 4.7 (18aa) 5.7 (22aa) 1.8 (7aa) 6.7 (26aa) 5.7 (22aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 2.6 (10aa) 1 (4aa) 3.6 (14aa) 2.8 (11aa) 4.1 (16aa) 3.4 (13aa) 3.4 (13aa) 7 (27aa) 3.6 (14aa) 4.7 (18aa)

>RER_24030 alkane-1-monooxygenase
ATGACGACCTTCGCGGAAGCATCCCGGACAGCGGCAGCGCACGCGCGGAACGGGCAGCGG
CCGGTGTGGCGAGACAAGAAGCGCTACCTGTGGCCACTGGGTTTGCTGGTTCCTCTCAGT
CCGTTCGCTGCGTGGTTCCTGGTCGACAAATTCAGTCTCGGGCTCTTCTGGGCCATCGGG
GCCTGGCTCATCGTGATCGTCATACCCCTGATCGATCTACTGGCGGGCGAGGACGGTAAC
AGCCCGCCGGACGAAGCGATCCCTGAATTGCAAGAGGATCGTTTCTACCGCTGGTGCACG
TATCTGTTCCTGCCCCTCCAATACGTCGGTCTCGTGTTCGTCGCCTGGTGCTGGGTGAAC
GCGCCGATGGCGGCGGGGGAGAAGTTCGCGCTCGCGATGACTGCCGGCGTGGTTGCCGGT
GTTGGGATCAACGCGGCACATGAATTGGGGCACAAGAGCGAAAAGCTCGAGAAGTGGCTC
GCGAAAGTAGCGCTGGCACAGTCCTTTTACGGCCATTTCTACGTCGAGCACAACCATGGT
CACCACGTCCGTGTCGCGACGCCGGAGGATCCGGCCAGTTCGCGATACAAGGAAAACTTC
TGGTTCTTCTTGCCGCGCAGTGTCATCGGCGGCCTGCGCTCGGGATGGCGGATCGAAAGC
GCTCGCCTTCAGCGTCAAGGCAGACGGACCTGGAGTTACCGCAACAACATCCTGAACGCG
TGGGCGATGAGTGTGGTTCTGTTCGGGAGTCTGATCGCCGCATTCGGGTGGGAAATCGCA
CCGTGGCTGGCCGTTCAGGCGATCGCCGGATTCACGTTCCTCGAGACCGCCAACTATCTC
GAGCACTACGGCTTGCTACGTGCGAAGCGGCCTGACGGCTCGTACGTTCGCTGCTCGCCC
GAGGACAGCTGGAACAGCGACCACGTCGTCAGCAATCTGTTCCTCTATCAACTTCAGCGT
CACAGTGACCATCACGCGAATCCGCGTCTGCGGTACCAGAGCCTGCGCAGTGCGGCCGAA
GCGCCGCAACTTCCAGCGGGTTACGCGGTCATGATCGTGTGCGCGTGGGTGCCGCCGCTG
TGGCGAAAGGTCATGGACAAGCGACTACTCGCCTACTACGACGGTGACATGACACGTATC
AACGTCCTGCCGGGTAAGTGA


>RER_24030 alkane-1-monooxygenase
MTTFAEASRTAAAHARNGQRPVWRDKKRYLWPLGLLVPLSPFAAWFLVDKFSLGLFWAIG
AWLIVIVIPLIDLLAGEDGNSPPDEAIPELQEDRFYRWCTYLFLPLQYVGLVFVAWCWVN
APMAAGEKFALAMTAGVVAGVGINAAHELGHKSEKLEKWLAKVALAQSFYGHFYVEHNHG
HHVRVATPEDPASSRYKENFWFFLPRSVIGGLRSGWRIESARLQRQGRRTWSYRNNILNA
WAMSVVLFGSLIAAFGWEIAPWLAVQAIAGFTFLETANYLEHYGLLRAKRPDGSYVRCSP
EDSWNSDHVVSNLFLYQLQRHSDHHANPRLRYQSLRSAAEAPQLPAGYAVMIVCAWVPPL
WRKVMDKRLLAYYDGDMTRINVLPGK

NBRC No.	clone name	start	stop	length(bp)
G12-001-101	REGR1S7017E07	2,560,218	2,602,480	42,263
G12-001-102	REGR1S8010A07	2,589,809	2,626,057	36,249

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.