CDS Information : RER_45970

Display	1000bp 10000bp 100000bp Clone

Rhodococcus erythropolis PR4 (= NBRC 100887) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Rhodococcus erythropolis PR4 (= NBRC 100887)
Replicon	chromosome
Start / Stop / Direction	5,038,943 / 5,037,912 / -
Location	complement(5037912..5038943)
Type	CDS
Length	1,032 bp (343 aa)

Product	acetoin dehydrogenase E1 component alpha subunit Synonym: acetoin--2,6-dichlorophenolindophenol oxidoreductase
Gene name	acoA
Functional category	Metabolism - Carbohydrate Metabolism
EC number	1.1.1.-
Note	May form a multienzyme complex with the E1 beta subunit (AcoB) (encoded by RER_45960), E2 component (AcoC) (encoded by RER_45950) and E3 component (AcoD) (encoded by Add9_044_0725).
KEGG pathway	map:00650
Sequence feature
References	Uniprot:Q48429 Pubmed:8206829

BLASTP Database:UniProtKB:14.0 show BLAST table	1 E=1e-130 Q48429_KLEPN Acetoin:DCPIP oxidoreductase alpha subunit Alignment 2 E=1e-125 B0VDZ5_ACIBY Acetoin:2,6-dichlorophenolindophenol oxidoreductase alpha subunit Acetoin:DCPIP oxidoreductase-alpha AO:DCPIP OR Alignment 3 E=1e-125 B2I0C2_ACIBC Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Alignment 4 E=1e-121 Q6FDF1_ACIAD Acetoin:2,6-dichlorophenolindophenol oxidoreductase alpha subunit Acetoin:DCPIP oxidoreductase-alpha AO:DCPIP OR Alignment 5 E=1e-104 Q1ATE7_RUBXD Pyruvate dehydrogenase Lipoamide Alignment
HAMAP	No significant hit
InterPro Database:interpro:18.0	IPR001017 Dehydrogenase, E1 component (Domain) PF00676   E1_dh
SignalP	No significant hit
TMHMM	No significant hit
SOSUI	No significant hit

size of protein	343 amino acids
molecular mass	36,006.42 Da
pI	5.23
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 14.9 (51aa) 7.3 (25aa) 6.4 (22aa) 4.4 (15aa) 3.8 (13aa) 0.3 (1aa) 3.2 (11aa) 2.9 (10aa) 11.4 (39aa) 5.8 (20aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 5.2 (18aa) 1.2 (4aa) 3.2 (11aa) 2.3 (8aa) 2.6 (9aa) 4.7 (16aa) 2.6 (9aa) 5 (17aa) 7.3 (25aa) 5.5 (19aa)

>RER_45970 acetoin dehydrogenase E1 component alpha subunit
GTGGTTCAACGGACGGCGCAGAAGCCGGCAACGTCAGGATCGAAAAAGACCGGGGCCAAG
CCTCATTCAGGGCTGGACCGGGATGCATTGCTGAAGGCGTACGAGAAGATGTCGCGCATC
CGCGCGTTCGAGGATCGATTGCACGAAGAGAATGCAACCGGTGACATCCCCGGCTTCATT
CACCTCTACTCGGGCCAGGAAGCCATCGCGGTGGGAGTGTGCGAGAACCTGAGCGACGCC
GACTACATCGGATCCACTCACCGCGGTCACGGGCACTGCATCGCCAAGGGCTGCGATCTG
AACGGCATGATGGCCGAGATCTTCGGCAAGGACGACGGCTTGTGCCGCGGTAAGGGTGGG
TCGATGCACATCGCCGACCTGTCCGTGGGAATGCTGGGTGCCAATGCGATTGTCGGCGGA
GCACCGTCGTTGGCGATCGGCGCTGCACTGAGTGGCAAGACACTTCGAAACGGCGTCATC
GCAGCGTCGTTCACGGGAGACGGTGGATCCAACCAGGGAACCGTCTTCGAGGCGATGAAC
ATGGCTGTGGTGCTGGATCTTCCGATCGTCTTCGTGATCGAGAACAACGGATTCGGTGAA
GCAACAGGAACCGACTACGCGGTGGGCGCACCCGACATCGCCGCCCGCGCTGCATCCTTC
GGAATGCCTGCCGTCAAGGTGGACGGCACCGACTTCTTCGCGGTCTACGACGCAATGGCG
GAAGCATCCGAGCGAGCCAGGACCGGCGGTGGCCCCACCACGATCGAGGCGGCCGCATTC
CGTTGGCACGGCCACTTCGAGGGTGACGCGCAGCTGTATCGCACGGCGGAACAGGTTGCG
CAACTGCGGGAAACCAAGGATCCGCTCAAGAATTTCCGTAGCAGTGTGGATGCGAAGAAG
GTCAGCACCGCTGATCTGGACGCGGTGGACGAGCAGTCGCGAGTGCTGGTGGACGAGGCG
GTGGCGAAGGCGCGCGCCGCAGCGTACCCACCAGTCGAAAATCTGCTGACCGATGTCTAC
GTGTCGTACTGA


>RER_45970 acetoin dehydrogenase E1 component alpha subunit
MVQRTAQKPATSGSKKTGAKPHSGLDRDALLKAYEKMSRIRAFEDRLHEENATGDIPGFI
HLYSGQEAIAVGVCENLSDADYIGSTHRGHGHCIAKGCDLNGMMAEIFGKDDGLCRGKGG
SMHIADLSVGMLGANAIVGGAPSLAIGAALSGKTLRNGVIAASFTGDGGSNQGTVFEAMN
MAVVLDLPIVFVIENNGFGEATGTDYAVGAPDIAARAASFGMPAVKVDGTDFFAVYDAMA
EASERARTGGGPTTIEAAAFRWHGHFEGDAQLYRTAEQVAQLRETKDPLKNFRSSVDAKK
VSTADLDAVDEQSRVLVDEAVAKARAAAYPPVENLLTDVYVSY

NBRC No.	clone name	start	stop	length(bp)
G12-001-199	REGR1S8017G11	5,017,871	5,050,980	33,110

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.