CDS Information : RER_46060

Display	1000bp 10000bp 100000bp Clone

Rhodococcus erythropolis PR4 (= NBRC 100887) chromosome	Legend Jump
Overview  Genome Clone

Center position		change
ID		change
Gene name		change

Organism	Rhodococcus erythropolis PR4 (= NBRC 100887)
Replicon	chromosome
Start / Stop / Direction	5,047,829 / 5,046,999 / -
Location	complement(5046999..5047829)
Type	CDS
Length	831 bp (276 aa)

Product	glycerol uptake facilitator protein
Gene name	glpF
Functional category	Environmental Information and Cellular Processing - Membrane Transport
EC number
Note	Belongs to the major intrinsic protein (MIP) family (TC 1.A.8).
KEGG pathway
Sequence feature
References	Uniprot:P0AER0 Pubmed:2152911,11226336

BLASTP Database:UniProtKB:14.0 show BLAST table	1 E=1e-106 A0ACS1_STRAM Putative transport integral membrane protein Alignment 2 E=1e-104 B1W5U3_STRGG Putative glycerol uptake facilitator protein Alignment 3 E=1e-103 Q826J3_STRAW Putative glycerol uptake facilitator protein Alignment 4 E=3e-97 A8M7K1_SALAI MIP family channel protein Alignment 5 E=5e-96 A4X280_SALTO MIP family channel protein Alignment
HAMAP	No significant hit
InterPro Database:interpro:18.0	IPR000425 Major intrinsic protein (Family) G3DSA:1.20.1080.10   no description SSF81338   Aquaporin-like PD000295   Q9AK60_STRCO_Q9AK60; PS00221   MIP PTHR19139   AQUAPORIN TRANSPORTER PF00230   MIP IPR012269 Aquaporin (Family) TIGR00861   MIP: MIP family channel proteins IPR022357 Major intrinsic protein, conserved site (Conserved_site) PS00221   MIP IPR023271 Aquaporin-like (Domain) SSF81338   Aquaporin-like G3DSA:1.20.1080.10   no description
SignalP	No significant hit
TMHMM	Transmembrane 6
SOSUI	Transmembrane 6

size of protein	276 amino acids
molecular mass	28,907.46 Da
pI	5.18
aa composition	Ala Val Leu Ile Phe Trp Pro Met Gly Ser 13 (36aa) 8.3 (23aa) 10.1 (28aa) 7.6 (21aa) 6.2 (17aa) 2.5 (7aa) 5.8 (16aa) 1.8 (5aa) 13.4 (37aa) 4.7 (13aa) Thr Cys Asn Gln Tyr Lys His Arg Asp Glu 6.2 (17aa) 0 (0aa) 2.9 (8aa) 1.8 (5aa) 2.9 (8aa) 1.4 (4aa) 1.1 (3aa) 3.6 (10aa) 3.6 (10aa) 2.9 (8aa)

>RER_46060 glycerol uptake facilitator protein
ATGGAGAAAGCCAAGCGATTGGGGCTTCCAGGCGAGTTGGCAGCCGAGTTCGCCGGAACG
ATGATCTTGATCTTGTTCGGCGTCGGAGTTGTGGCGCAGGTTGTGTCCGGCGGGAGCGGT
GGGGGTCTCGGCGGACACGACAGTATTGCGTGGGCCTGGGGACTCGGCGTCATGTTCGGC
ATCTATGTCGCAGGCCGGGTGACGGGTGCTCATCTGAACCCCGCAGTGACCTTCGCCTTC
GCGATCTTCCGGGGATTCTCCTGGCGCAAGGTGCTGCCGTACACCCTGGCGCAGACCGCT
GGAGCCTTTGTCGCCGCGTTGATAGTCCGGTGGAATTACAACGACATGCTCAATGCGATC
GATCCCGGACACACAACCGCCACCCAGACGATCTTCTCGACGATGCCCGGAAACGGAACG
CTTCCGGTCAGTCTCGGCTCGGCTCTGATCGATCAGATCATCGGCACCGCCATCCTGTTG
TTCCTCATCGTTGCAGTCACCGATTCTCGTGGAACTCCGCCGCTCGCGAATCTCGCGCCG
TTTGTCGTCGGATTGATCGTGGTGGGAATCGGATTCGCCTGGGCGACCAACGCGGGGTAC
GCCATCAACCCGGCGCGCGACTTCGGGCCACGACTGGCGTCCTTCATCACCGGCTACGGC
AGCGCCTGGCGAGATCAGTACGGGAGCTTGTATTTCTGGGTGCCCATCGTGGGACCCCTG
ATCGGCGCTCCGATCGGAGTGTTCCTCTACGACAAGCTGGTTGGAAGATTCCTGCCTGAC
GACGAGTCGGCGGAGGAAGCGACCGAAGAGCTGGCGTCGATACCGGCGTGA


>RER_46060 glycerol uptake facilitator protein
MEKAKRLGLPGELAAEFAGTMILILFGVGVVAQVVSGGSGGGLGGHDSIAWAWGLGVMFG
IYVAGRVTGAHLNPAVTFAFAIFRGFSWRKVLPYTLAQTAGAFVAALIVRWNYNDMLNAI
DPGHTTATQTIFSTMPGNGTLPVSLGSALIDQIIGTAILLFLIVAVTDSRGTPPLANLAP
FVVGLIVVGIGFAWATNAGYAINPARDFGPRLASFITGYGSAWRDQYGSLYFWVPIVGPL
IGAPIGVFLYDKLVGRFLPDDESAEEATEELASIPA

NBRC No.	clone name	start	stop	length(bp)
G12-001-199	REGR1S8017G11	5,017,871	5,050,980	33,110
G12-001-200	REGR1S7007C04	5,042,142	5,085,203	43,062

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.