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CDS Information : ROP_16400

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close this sectionGenomic map

Display Clone

Rhodococcus opacus B4 chromosome

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Overview 
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Proteome 
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close this sectionLocation

Organism Rhodococcus opacus B4
Replicon chromosome
Start / Stop / Direction 1,751,393 / 1,747,887 / -
Location complement(1747887..1751393)
Type CDS
Length 3,507 bp (1,168 aa)

close this sectionAnnotation

Product DNA-directed RNA polymerase beta subunit
Gene name rpoB
Functional category Genetic Information Processing
- Transcription
EC number 2.7.7.6
Note May form a complex with the alpha chain (encoded by ROP_62150), beta' chain (encoded by ROP_16390) and omega chain (encoded by ROP_69370).
KEGG pathway map:03020 KO:K03043
Sequence feature
References HAMAP:MF_01321

close this sectionProteome

Proteome results
strainO (1DE-LC)
strainR (1DE-LC)
strainD (1DE-LC)
strainO+OA (1DE-LC)
Show sequence

close this sectionComputational search results

BLASTP
Database:UniProtKB:14.0
1E=0RPOB_RHOSRDNA-directed RNA polymerase subunit beta
RNAP subunit beta
Transcriptase subunit beta
RNA polymerase subunit beta
Alignment
2E=0RPOB_MYCSKDNA-directed RNA polymerase subunit beta
RNAP subunit beta
Transcriptase subunit beta
RNA polymerase subunit beta
Alignment
3E=0RPOB_MYCSSDNA-directed RNA polymerase subunit beta
RNAP subunit beta
Transcriptase subunit beta
RNA polymerase subunit beta
Alignment
4E=0RPOB2_NOCFADNA-directed RNA polymerase subunit beta 2
RNAP subunit beta 2
Transcriptase subunit beta 2
RNA polymerase subunit beta 2
Alignment
5E=0RPOB_MYCSJDNA-directed RNA polymerase subunit beta
RNAP subunit beta
Transcriptase subunit beta
RNA polymerase subunit beta
Alignment
HAMAP
Database:HAMAP:20080521
 [26-1129]  12.146 MF_01321
MF_01321   RPOB
InterPro
Database:interpro:18.0
IPR007120 RNA polymerase Rpb2, domain 6 (Domain)
 [645-1037]  1.1e-64 G3DSA:2.40.270.10
G3DSA:2.40.270.10   no description
 [628-1051]  2.2e-196 PF00562
PF00562   RNA_pol_Rpb2_6
IPR007121 RNA polymerase, beta subunit, conserved site (Conserved_site)
 [869-881]  PS01166
PS01166   RNA_POL_BETA
IPR007121 RNA polymerase, beta subunit, C-terminal (Domain)
 [869-881]  PS01166
PS01166   RNA_POL_BETA
IPR007121 RNA polymerase, beta subunit (Family)
 [869-881]  PS01166
PS01166   RNA_POL_BETA
IPR007641 RNA polymerase Rpb2, domain 7 (Domain)
 [1053-1129]  1.3e-50 PF04560
PF04560   RNA_pol_Rpb2_7
IPR007642 RNA polymerase Rpb2, domain 2 (Domain)
 [168-355]  2.4e-06 PF04561
PF04561   RNA_pol_Rpb2_2
IPR007644 RNA polymerase, beta subunit, protrusion (Domain)
 [39-421]  5.8e-40 PF04563
PF04563   RNA_pol_Rpb2_1
IPR007645 RNA polymerase Rpb2, domain 3 (Domain)
 [425-494]  1.5e-42 PF04565
PF04565   RNA_pol_Rpb2_3
IPR010243 DNA-directed RNA polymerase, beta subunit (Family)
 [401-1147]  PTHR20856:SF3
PTHR20856:SF3   DNA-DIRECTED RNA POLYMERASE BETA CHAIN
 [21-1126]  TIGR02013
TIGR02013   rpoB: DNA-directed RNA polymerase, beta sub
IPR015712 DNA-directed RNA polymerase I, subunit 2 (Domain)
 [401-1147]  PTHR20856
PTHR20856   DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2
IPR015712 DNA-directed RNA polymerase, subunit 2 (Family)
 [401-1147]  PTHR20856
PTHR20856   DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2
SignalP No significant hit
TMHMM No significant hit
SOSUI No significant hit

close this sectionCalculated information (Amino acid sequence)

size of protein 1168 amino acids
molecular mass 128,199.00 Da
pI 4.65
aa composition
AlaValLeuIlePheTrpProMetGlySer
7.6
(89aa)
8.6
(100aa)
8.4
(98aa)
6.1
(71aa)
3
(35aa)
0.8
(9aa)
5
(58aa)
2.7
(31aa)
9
(105aa)
5.4
(63aa)
ThrCysAsnGlnTyrLysHisArgAspGlu
5.7
(67aa)
0.5
(6aa)
3.3
(38aa)
3.4
(40aa)
1.9
(22aa)
4.5
(52aa)
1.7
(20aa)
6.8
(79aa)
7.7
(90aa)
8.1
(95aa)

open this sectionSequence

close this sectionCovered clones

NBRC No. clone name start stop length(bp)
G13-001-067 RO1FO01L80010P05 1,724,931 1,769,182 44,252

close this sectionDistribution of Our Microbial Genomic DNA clones

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.