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CDS Information : ROP_69510

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close this sectionGenomic map

Display Clone

Rhodococcus opacus B4 (= NBRC 108011) chromosome

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Genome
Proteome 
Clone

close this sectionLocation

Organism Rhodococcus opacus B4 (= NBRC 108011)
Replicon chromosome
Start / Stop / Direction 7,580,190 / 7,581,443 / +
Location 7580190..7581443
Type CDS
Length 1,254 bp (417 aa)

close this sectionAnnotation

Product 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II
Synonym: riboflavin biosynthesis protein RibA
Gene name ribA
Functional category Metabolism
- Metabolism of Cofactors and Vitamins
EC number 3.5.4.25
Note bifunctional protein
KEGG pathway map:00740 KO:K02858,K01497
Sequence feature
References HAMAP:MF_00179

close this sectionProteome

Proteome results
strainO (1DE-LC)
strainR (1DE-LC)
strainD (1DE-LC)
strainO+OA (1DE-LC)
Show sequence

close this sectionComputational search results

BLASTP
Database:UniProtKB:14.0
1E=0RIBBA_RHOSRRiboflavin biosynthesis protein ribBAAlignment
2E=0RIBBA_NOCFARiboflavin biosynthesis protein ribBAAlignment
3E=0Q1B9D6_MYCSSGTP cyclohydrolase IIAlignment
4E=0A1UFM7_MYCSKGTP cyclohydrolase IIAlignment
5E=0A3PZ88_MYCSJGTP cyclohydrolase IIAlignment
HAMAP
Database:HAMAP:20080521
 [207-407]  40.016 MF_00179 [207-407]  40.016 MF_00179
MF_00179   RIBA
 [2-206]  37.317 MF_00180 [2-206]  37.317 MF_00180
MF_00180   RIBB
 [3-403]  56.352 MF_01283
MF_01283   RIBBA
InterPro
Database:interpro:18.0
IPR000422 3,4-Dihydroxy-2-butanone 4-phosphate synthase (Domain)
 [7-204]  1.8e-129 PF00926
PF00926   DHBP_synthase
 [2-206]  7e-92 PD003034
PD003034   Q741F1_MYCPA_Q741F1;
 [3-202]  4.4e-94 TIGR00506
TIGR00506   ribB: 3,4-dihydroxy-2-butanone 4-phosphate
IPR000422 3,4-dihydroxy-2-butanone 4-phosphate synthase, RibB (Family)
 [7-204]  1.8e-129 PF00926
PF00926   DHBP_synthase
 [3-202]  4.4e-94 TIGR00506
TIGR00506   ribB: 3,4-dihydroxy-2-butanone 4-phosphate
IPR000926 GTP cyclohydrolase II (Domain)
 [209-382]  3.6e-120 PF00925
PF00925   GTP_cyclohydro2
 [210-405]  1.1e-101 TIGR00505
TIGR00505   ribA: GTP cyclohydrolase II
IPR000926 GTP cyclohydrolase II, RibA (Family)
 [210-405]  1.1e-101 TIGR00505
TIGR00505   ribA: GTP cyclohydrolase II
 [209-382]  3.6e-120 PF00925
PF00925   GTP_cyclohydro2
IPR016299 Riboflavin biosynthesis protein RibA (Family)
 [1-410]  1.1e-203 PIRSF001259
PIRSF001259   GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase
IPR017945 DHBP synthase RibB-like alpha/beta domain (Domain)
 [4-215]  3.3e-82 G3DSA:3.90.870.10
G3DSA:3.90.870.10   no description
 [2-206]  6.8e-85 SSF55821
SSF55821   YrdC/RibB
SignalP No significant hit
TMHMM No significant hit
SOSUI No significant hit

close this sectionCalculated information (Amino acid sequence)

size of protein 417 amino acids
molecular mass 45,184.00 Da
pI 4.78
aa composition
AlaValLeuIlePheTrpProMetGlySer
11
(46aa)
8.2
(34aa)
10.1
(42aa)
4.3
(18aa)
1.9
(8aa)
0.2
(1aa)
3.8
(16aa)
3.1
(13aa)
10.1
(42aa)
3.8
(16aa)
ThrCysAsnGlnTyrLysHisArgAspGlu
5
(21aa)
1.4
(6aa)
1.7
(7aa)
2.6
(11aa)
2.6
(11aa)
2.4
(10aa)
3.1
(13aa)
8.4
(35aa)
10.3
(43aa)
5.8
(24aa)

open this sectionSequence

close this sectionCovered clones

NBRC No. clone name start stop length(bp)
G13-001-287 RO1FO01L80011L12 7,559,379 7,600,855 41,477

close this sectionDistribution of Our Microbial Genomic DNA clones

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.