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CDS Information : ROP_69670

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close this sectionGenomic map

Display Clone

Rhodococcus opacus B4 (= NBRC 108011) chromosome

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Overview 
Genome
Proteome 
Clone

close this sectionLocation

Organism Rhodococcus opacus B4 (= NBRC 108011)
Replicon chromosome
Start / Stop / Direction 7,600,826 / 7,598,718 / -
Location complement(7598718..7600826)
Type CDS
Length 2,109 bp (702 aa)

close this sectionAnnotation

Product transketolase
Gene name tkt
Functional category Metabolism
- Carbohydrate Metabolism
EC number 2.2.1.1
Note
KEGG pathway map:00030 KO:K00615
Sequence feature
References Uniprot:P33570 Pubmed:8396116

close this sectionProteome

Proteome results
strainO (2D-PAGE)
strainR (2D-PAGE)
strainD (2D-PAGE)
strainO+OA (2D-PAGE)
strainO (1DE-LC)
strainR (1DE-LC)
strainD (1DE-LC)
strainO+OA (1DE-LC)
Show sequence

close this sectionComputational search results

BLASTP
Database:UniProtKB:14.0
1E=0Q0S0I6_RHOSRTransketolaseAlignment
2E=0Q5YTS0_NOCFAPutative transketolaseAlignment
3E=0B1MC67_MYCABProbable transketolaseAlignment
4E=0A0QWY0_MYCS2TransketolaseAlignment
5E=0A4FBP1_SACENTransketolaseAlignment
HAMAP No significant hit
InterPro
Database:interpro:18.0
IPR005474 Transketolase, N-terminal (Domain)
 [31-51]  PS00801
PS00801   TRANSKETOLASE_1
 [22-358]  2e-188 PF00456
PF00456   Transketolase_N
IPR005475 Transketolase, central region (Domain)
 [497-513]  PS00802
PS00802   TRANSKETOLASE_2
 [373-560]  3.4e-71 PF02779
PF02779   Transket_pyr
IPR005476 Transketolase, C-terminal (Domain)
 [573-689]  0.0015 PF02780
PF02780   Transketolase_C
IPR005478 Bacterial transketolase (Family)
 [35-692]  PTHR11624:SF1
PTHR11624:SF1   TRANSKETOLASE
 [24-698]  TIGR00232
TIGR00232   tktlase_bact: transketolase
IPR009014 Transketolase, C-terminal-like (Domain)
 [571-697]  3.6e-40 G3DSA:3.40.50.920
G3DSA:3.40.50.920   no description
 [561-699]  6.9e-42 SSF52922
SSF52922   TK C-terminal domain-like
IPR020826 Transketolase binding site (Binding_site)
 [497-513]  PS00802
PS00802   TRANSKETOLASE_2
SignalP No significant hit
TMHMM No significant hit
SOSUI No significant hit

close this sectionCalculated information (Amino acid sequence)

size of protein 702 amino acids
molecular mass 75,127.63 Da
pI 4.91
aa composition
AlaValLeuIlePheTrpProMetGlySer
13.5
(95aa)
7.8
(55aa)
8.8
(62aa)
4.7
(33aa)
2.4
(17aa)
2
(14aa)
5.3
(37aa)
1.6
(11aa)
9.8
(69aa)
4.8
(34aa)
ThrCysAsnGlnTyrLysHisArgAspGlu
6.8
(48aa)
0.3
(2aa)
1.7
(12aa)
2.6
(18aa)
2.7
(19aa)
3.3
(23aa)
3.1
(22aa)
5.4
(38aa)
6.4
(45aa)
6.8
(48aa)

open this sectionSequence

close this sectionCovered clones

NBRC No. clone name start stop length(bp)
G13-001-287 RO1FO01L80011L12 7,559,379 7,600,855 41,477
G13-001-288 RO1FO01L80010E15 7,590,173 7,629,376 39,204

close this sectionDistribution of Our Microbial Genomic DNA clones

We have been distributing copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing projects.
You can find more detailed information at http://www.nbrc.nite.go.jp/e/mdna-e.html.