Ichikawa, N., Oguchi, A., Ikeda, H., Ishikawa, J., Kitani, S., Watanabe, Y., Nakamura, S., Katano, Y., Kishi, E., Sasagawa, M., Ankai, A., Fukui, S., Hashimoto, Y., Kamata, S., Otoguro, M., Tanikawa, S., Nihira, T., Horinouchi, S., Ohnishi, Y., Hayakawa, M., Kuzuyama, T., Arisawa, A., Nomoto, F., Miura, H., Takahashi, Y., Fujita, N.
DNA research Abstract
Comment
|
| Abstract: Kitasatospora setae NBRC 14216T (= KM-6054T ) is known to produce setamycin
(bafilomycin B1) possessing antitrichomonal activity. The genus Kitasatospora is
morphologically similar to the genus Streptomyces, although they are distinguishable
from each other on the basis of cell wall composition and 16S rDNA sequence. We have
determined the complete genome sequence of K. setae NBRC 14216T as the first
Streptomycetaceae genome other than Streptomyces. The genome is a single linear
chromosome of 8,783,278 bp with terminal inverted repeats of 127,148 bp, predicted to
encode 7,569 protein coding genes, 9 rRNA operons, 1 tmRNA and 74 tRNA genes.
While these features resemble those of Streptomyces, genome-wide comparison of
orthologous genes between K. setae and Streptomyces revealed smaller extent of
synteny. Multi-locus phylogenetic analysis based on amino acid sequences
unequivocally placed K. setae outside the Streptomyces genus. Although many of the
genes related to morphological differentiation identified in Streptomyces were highly
conserved in K. setae, there were some differences such as the apparent absence of the
AmfS (SapB) class of surfactant protein and differences in the copy number and
variation of paralogous components involved in cell wall synthesis.
|
