| ID |
NRULE_0001 |
| 遺伝子ID |
NHAA |
| 作成日 |
2012/06/29 |
| タンパク質名 |
Protein name
- Full
- Nitrile hydratase subunit alpha
- Short
- NHase
- EC
- EC 4.2.1.84
|
| 遺伝子名 |
nthA |
| 遺伝子条件 |
PFAM; PF02979; hit=1ANDTIGRFAMs; TIGR01323; hit=1 |
| 参照 |
Gene Ontology
- GO
- GO:0046914, F:transition metal ion binding, IEA:InterPro
- GO:0006807, P:nitrogen compound metabolic process, IEA:InterPro
Family and domain databases
- InterPro
- IPR004232, CN_Hdrtase_a/SCN_Hdrlase_g
- IPR023900, CN_Hdrtase_asu/SCN_Hdrlase_gsu
- IPR018141, Nitrile_hydratase_asu
- Pfam
- PF02979, NHase_alpha, 1
- PIRSF
- PIRSF001426, NHase_alpha, 1
- ProDom
- PD007559, CN_Hdrtase_asu/SCN_Hdrlase_gsu, 1
- TIGRFAMs
- TIGR01323, Nitrile_alph, 1
|
| 配列の特徴 |
P13448 Rhodococcus erythropolis (Arthrobacter picolinophilus).
| METAL | 110 | Iron. |
| METAL | 113 | Iron. |
| METAL | 114 | Iron. |
| METAL | 115 | Iron. |
| MOD_RES | 113 | Cys teine sulfinic acid (-SO2H). |
| MOD_RES | 115 | Cysteine sulfenic acid (-SOH). |
P21219 Rhodococcus rhodochrous.
| METAL | 102 | Cobalt (By similarity). |
| METAL | 105 | Cobalt (By similarity). |
| METAL | 106 | Cobalt (By similarity). |
| METAL | 107 | Cobalt (By similarity). |
|
| 注釈 |
- Function
- NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class
- Catalytic activity
- An aliphatic amide = a nitrile + H(2)O
- Subunit structure
- Heterodimer of an alpha and a beta chain
- Sequence similarities
- Belongs to the nitrile hydratase subunit alpha family
|
| 参考文献 |
- P13448 Rhodococcus erythropolis (Arthrobacter picolinophilus).
- 2659343
- Primary structure of nitrile hydratase deduced from the nucleotide sequence of a Rhodococcus species and its expression in Escherichia coli. (Eur J Biochem. 1989)
- 10101282
- Functional expression of nitrile hydratase in Escherichia coli: requirement of a nitrile hydratase activator and post-translational modification of a ligand cysteine. (J Biochem. 1999)
- P21219 Rhodococcus rhodochrous.
- 8633053
- Characterization of the gene cluster of high-molecular-mass nitrile hydratase (H-NHase) induced by its reaction product in Rhodococcus rhodochrous J1. (Proc Natl Acad Sci U S A. 1996)
- P29378 Rhodococcus rhodochrous.
- 1840499
- Cloning, nucleotide sequence and expression in Escherichia coli of two cobalt-containing nitrile hydratase genes from Rhodococcus rhodochrous J1. (Biochim Biophys Acta. 1991)
|
