遺伝子 : Serine/alanine racemase (VANT)

遺伝子情報

ID	NRULE_0269
遺伝子ID	VANT
作成日	2014/07/04
タンパク質名	Protein name Full Serine/alanine racemase EC EC 5.1.1.-
遺伝子名	vanT
遺伝子条件	HAMAP; MF_01201; hit=1ANDBLASTP; Q9X3P3; hit=1; score=1400; pid=45
参照	Gene Ontology GO GO:0016021, C:integral component of membrane, IEA:UniProtKB-KW GO:0005886, C:plasma membrane, IEA:UniProtKB-SubCell GO:0008784, F:alanine racemase activity, IEA:UniProtKB-HAMAP GO:0030170, F:pyridoxal phosphate binding, IEA:UniProtKB-HAMAP GO:0016747, F:transferase activity, transferring acyl groups other than amino-acyl groups, IEA:InterPro GO:0030632, P:D-alanine biosynthetic process, IEA:UniProtKB-UniPathway GO:0009252, P:peptidoglycan biosynthetic process, IEA:UniProtKB-KW GO:0008360, P:regulation of cell shape, IEA:UniProtKB-KW GO:0046677, P:response to antibiotic, IEA:UniProtKB-KW Enzyme and pathway databases BioCyc MetaCyc:MONOMER-15476, - UniPathway UPA00042, UER00497 Family and domain databases HAMAP MF_01201, Ala_racemase, 1 InterPro IPR002656, Acyl_transf_3 IPR000821, Ala_racemase IPR009006, Ala_racemase/Decarboxylase_C IPR011079, Ala_racemase_C IPR001608, Ala_racemase_N IPR020622, Ala_racemase_pyridoxalP-BS IPR029066, PLP-binding_barrel IPR011248, Serine/alanine_racemase Pfam PF01757, Acyl_transf_3, 1 PF00842, Ala_racemase_C, 1 PF01168, Ala_racemase_N, 1 PIRSF PIRSF036464, Ser_ala_racem, 1 PRINTS PR00992, ALARACEMASE SMART SM01005, Ala_racemase_C, 1 TIGRFAMs TIGR00492, alr, 1 PROSITE PS00395, ALANINE_RACEMASE, 1
配列の特徴	Q9X3P3 Enterococcus gallinarum. ACT_SITE 371 Proton acceptor (By similarity). ACT_SITE 597 Proton acceptor (By similarity). BINDING 465 Substrate (By similarity). BINDING 646 Substrate; via amide nitrogen (By similarity). MOD_RES 371 N6-(pyridoxal phosphate)lysine (By similarity).
注釈	Function Catalyzes the interconversion of L-serine and D-serine, and L-alanine and D-alanine Catalytic activity L-alanine = D-alanine L-serine = D-serine Cofactor Pyridoxal phosphate (By similarity) Pathway Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1 Subunit structure Homodimer (Probable) Domain The cytoplasmic C-terminal domain is responsible for the racemase activity Sequence similarities In the N-terminal section; belongs to the acyltransferase 3 family In the C-terminal section; belongs to the alanine racemase family Note The vanL gene cluster is similar in organization to the vanC operon, but the VanT serine racemase is encoded by two separate genes, vanTm(L)(membrane binding) and vanTr(L)(racemase). These genes are not contained in this rule
参考文献	Q9X3P3 Enterococcus gallinarum. 10209740 Characterization and modelling of VanT: a novel, membrane-bound, serine racemase from vancomycin-resistant Enterococcus gallinarum BM4174. (Mol Microbiol. 1999) 10878136 Serine and alanine racemase activities of VanT: a protein necessary for vancomycin resistance in Enterococcus gallinarum BM4174. (Microbiology (Reading). 2000) 12615855 Role of the transmembrane domain of the VanT serine racemase in resistance to vancomycin in Enterococcus gallinarum BM4174. (J Antimicrob Chemother. 2003)

関与する機能

機能名
Vancomycin resistance (via D-Ala-D-Ser precursor)

該当CDS

学名	NBRC番号	CDS	タンパク質名
Enterococcus gilvus	100696	I592_01345	alanine racemase
Enterococcus pallens	100697	I588_03702	alanine racemase