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Members of the phylum Chloroflexi represent a wide variety of phenotypes, and distributed widely throughout the environment. The phylum Chloroflexi is currently divided into at least five classes based on 16S rRNA genes.
Anaerolinea thermophila UNI-1T（= NBRC 100420T）belongs to the class Anaerolineae containing a number of diverse environmental 16S rRNA gene sequences with only a few cultured strains. A. thermophila UNI-1T is the first cultivated strain in this class.
A. thermophila UNI-1T was isolated from a thermophilic upflow anaerobic sludge blanket reactor treating fried soybean-curd manufacturing waste water. A. thermophila UNI-1T is a strictly anaerobic, filamentous (longer than 100 ㎛ and 0.2 to 0.3 ㎛) bacterium that grows chemo-organotrophically on a number of carbohydrates and amino acids in the presence of yeast extract.
The genome of A. thermophila UNI-1T consisted of a single circular chromosome (3,532,378 bp; G+C content of 53.8%) containing 3,179 predicted protein-coding genes. The reconstructed central metabolic pathways from the A. thermophila UNI-1T genome showed that a lot of biosynthesis pathways whose products are essential for growth, such as nucleotide, methionine, fatty acid, vitamin and coenzyme seemed to be incomplete. Remarkably, the biosynthesis pathway of fatty acid seemed to lack genes necessary for the chain elongation. On the other hand, the genome encodes unique genes and pathways which have been mainly described for archaea. These analysis data would provide insights into the understanding of Anaerolineae species and their ecological environment. |
Courtesy of
Dr. Sekiguchi (AIST) |
References:
[1]
Syntrophothermus lipocalidus gen. nov., sp. nov., a novel thermophilic, syntrophic, fatty-acid-oxidizing anaerobe which utilizes isobutyrate.
Sekiguchi, Y., Kamagata, Y., Nakamura, K., Ohashi, A., Harada, H. (2000)
Int. J. Syst. Evol. Microbiol. 50:771-779.
[PMID:10758888]
[2]
In situ detection, isolation, and physiological properties of a thin filamentous microorganism abundant in methanogenic granular sludges: a novel isolate affiliated with a clone cluster, the green non-sulfur bacteria, subdivision I.
Sekiguchi, Y., Takahashi, H., Kamagata, Y., Ohashi, A., Harada, H. (2001)
Appl. Environ. Microbiol. 67:5740-5749
[PMID:11722931]
[3]
Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level.
Sekiguchi, Y., Yamada, T., Hanada, S., Ohashi, A., Harada, H., Kamagata, Y. (2003)
Int. J. Syst. Evol. Microbiol. 53:1843-1851
[PMID:14657113]
[4]
Characterization of filamentous bacteria, belonging to candidate phylum KSB3, that are associated with bulking in methanogenic granular sludges.
Yamada T, Yamauchi T, Shiraishi K, Hugenholtz P, Ohashi A, Harada H, Kamagata Y, Nakamura K, Sekiguchi Y.(2007)
ISME J. 3:246-255
[PMID:18043635]
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| Genomic size |
3,532,378 bp |
| The number of ORFs |
3,177 |
| GC content |
53.85 % |
| Genome Database |
DOGAN |
| NBRC No. |
100420 |
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Distribution of Our Microbial Genomic DNA clones
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At the Department of Biotechnology of the National Institute of Technology
and Evaluation (NITE-DOB, an Incorporated Administrative Agency), we have
been distributing to both academia and industries copies of the microbial
genomic DNA clones constructed during the course of each of the genomic
DNA sequencing project. |
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NITE TOP >
Biotechnology Field >
Genome Analysis Center >
Genome Projects >
Anaerolinea thermophila UNI-1
