Providing genomic information /Genome Projects
Staphylococcus aureus N315
Staphylococcus aureus N315 was isolated as an MRSA (methicillin-resistant S. aureus) that was a major pathogen causing hospital-acquired infections in 1982. This bacterium is easy to acquire antibiotic-resistance, even to vancomycin that is said to be a last hope of drug to cure MRSA-infected patients. Analysis of the genome of S. aureus N315 is expected to enhance the accuracy of detection of Staphylococcal infections as well as to reduce the time for detection. Furthermore, it is expected that the mechanisms of antibiotic-resistance will be further investigated so as to develop new methods and techniques to combat with MRSA.
Results of the genome analysis of S. aureus N315 indicate that it contains a plasmid of 25 kb in size. Its genome size is 2.81 Mb with a 32.8 % G+C content, 20 copies of insertion sequences and five transposons. There are three 'pathogenicity islands' within the genome. With a few characteristic exceptions such as the genes involved in sporulation in Bacillus subtilis or those involved in toxin production in S. aureus N315, the numbers of genes in various functional categories are very similar between the two bacteria.
Dr. Hiramatsu (Juntendo univ.)
|Genomic size:||2,839,469 bp|
|The number of ORFs:||2,593|
|Published paper:||Whole genome sequencing of methicillin-resistant Staphylococcus aureus.|
|The Lancet 357(9264), 1225-1240 (2001)|
At the Department of Biotechnology of the National Institute of Technology and Evaluation (NITE-DOB, an Incorporated Administrative Agency), we have been distributing to both academia and industries copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing project.