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Cluster information : Vicenistatin


close this sectionCompound

Entry nameVicenistatin
PKS TypeTypeI modular
Starter Unit3-amino-2-methylpropionyl-ACP
Chain Length9
Sugar Unitvicenisamine
ClassificationMacrocyclic Lactam
ActivityAntitumor
CompositionC30H48N2O4
Vicenistatin

close this sectionOriginal source

Organism
StrainHC34
Contig
Click on the icon to see Genetic map.

close this sectionPKS/NRPS Module

0
Vicen_00200
vinP1
1 malonyl-CoA
2 malonyl-CoA
3 methylmalonyl-CoA
Vicen_00050
vinP2
4 methylmalonyl-CoA
Vicen_00060
vinP3
5 malonyl-CoA
6 methylmalonyl-CoA
Vicen_00070
vinP4
7 malonyl-CoA
8 malonyl-CoA
A9..410
PCP2..59
AT46..138
ACP41..114
KS137..513
AT668..989
DH1035..1194
ER1526..1831
KR1841..2019
ACP2122..2192
KS2220..2595
AT2749..3063
DH3109..3272
KR3592..3770
ACP3873..3943
KS3966..4342
AT4499..4832
DH4881..5045
KR5386..5566
ACP5671..5741
KS34..409
AT593..926
DH975..1139
ER1483..1797
KR1807..1987
ACP2092..2162
KS33..409
AT565..882
DH928..1091
KR1369..1547
ACP1654..1724
KS1748..2109
AT2273..2601
KR2916..3097
ACP3203..3273
KS34..409
AT569..886
DH932..1094
KR1380..1558
ACP1664..1734
KS1759..2134
AT2295..2612
DH2658..2824
KR3148..3328
ACP3423..3497
TE3577..3804

close this sectionReference

Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii.
Ogasawara Y, Katayama K, Minami A, Otsuka M, Eguchi T, Kakinuma K
[PMID: 15112997]Chem Biol. 11 (2004) 79-86
Involvement of glutamate mutase in the biosynthesis of the unique starter unit of the macrolactam polyketide antibiotic vicenistatin.
Ogasawara Y, Kakinuma K, Eguchi T
[PMID: 16161486]J Antibiot (Tokyo). 58 (2005) 468-72
Substrate flexibility of vicenisaminyltransferase VinC involved in the biosynthesis of vicenistatin.
Minami A, Eguchi T
[PMID: 17388594]J Am Chem Soc. 129 (2007) 5102-7
A natural protecting group strategy to carry an amino acid starter unit in the biosynthesis of macrolactam polyketide antibiotics.
Shinohara Y, Kudo F, Eguchi T
[PMID: 22010945]J Am Chem Soc. 133 (2011) 18134-7
The crystal structure of the amidohydrolase VinJ shows a unique hydrophobic tunnel for its interaction with polyketide substrates.
Shinohara Y, Miyanaga A, Kudo F, Eguchi T
[PMID: 24530530]FEBS Lett. (2014)
The crystal structure of the adenylation enzyme VinN reveals a unique beta-amino acid recognition mechanism.
Miyanaga A, Cieslak J, Shinohara Y, Kudo F, Eguchi T
[PMID: 25246523]J Biol Chem. 289 (2014) 31448-57
Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis.
Miyanaga A, Iwasawa S, Shinohara Y, Kudo F, Eguchi T
[PMID: 26831085]Proc Natl Acad Sci U S A. 113 (2016) 1802-7

close this sectionData download

Compound nameGenomic
nucleotide
sequence
Nucleotide
sequences of the
coding regions
Translation of the
coding sequences
List of genes/ORFsGBK
Vicenistatin
Genomic nucleotide sequence
65 KB
Nucleotide sequences of the coding regions
64 KB
Translation of the coding sequences
22 KB
List of genes/ORFs
3 KB

 

 
GenBank format
124 KB

close this sectionHistory

  • 2016-12-27[Update]
  • 2015-02-12[Update]
  • 2014-03-10[Update]
  • 2013-09-04[Update]
  • 2012-06-04[Release]