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Cluster information : Azinomycin B


close this sectionCompound

Entry nameAzinomycin B
Moiety descriptionA: 3-methoxy-5-methyl-naphthoic acid unit
B: skeleton assembly
PKS TypeA: other
B: NRPS
Starter UnitA: acetyl-CoA
Chain LengthA: 6
Sugar Unitnone
Classification
ActivityAntitumor
CompositionC31H33N3O11
Azinomycin B

close this sectionOriginal source

Organism
StrainNRRL 2485 (=NBRC 13928)
Contig
Click on the icon to see Genetic map.

close this sectionPKS/NRPS Module

AAzino_00260
aziB
1 acetyl-CoA
malonyl-CoA
Azino_00140
aziG
2
BAzino_00080
aziA1
1 3-methoxy-5-methyl-naphthoic acid
Azino_00100
aziA2
2
3
Azino_00020
aziA3
3 alpha-ketoisovaleric acid
Azino_00040
aziA4
4 aziridino[1, 2a]pyrrolidinyl amino acid
Azino_00250
aziA5
5 threonine
KS11..383
AT546..858
TH865..1017
KR1389..1582
ACP1666..1739
CEC1..133
A44..457
PCP540..607
C2..303
PCP551..622
C671..969
A115..556
KR871..1060
PCP1195..1262
C35..331
A515..908
PCP991..1062
C64..360
A549..960
PCP1047..1114
R1166..1404

close this sectionReference

Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis.
Zhao Q, He Q, Ding W, Tang M, Kang Q, Yu Y, Deng W, Zhang Q, Fang J, Tang G, Liu W
[PMID: 18635006]Chem Biol. 15 (2008) 693-705
Biosynthesis of 3-methoxy-5-methyl naphthoic acid and its incorporation into the antitumor antibiotic azinomycin B.
Ding W, Deng W, Tang M, Zhang Q, Tang G, Bi Y, Liu W
[PMID: 20485749]Mol Biosyst. 6 (2010) 1071-81
Essential role of an unknown gene aziU3 in the production of antitumor antibiotic azinomycin B verified by utilizing optimized genetic manipulation systems for Streptomyces sahachiroi.
Wang S, Zhao R, Liu K, Zhu M, Li A, He J
[PMID: 23039858]FEMS Microbiol Lett. 337 (2012) 147-54
Characterization of a novel DNA glycosylase from S. sahachiroi involved in the reduction and repair of azinomycin B induced DNA damage.
Wang S, Liu K, Xiao L, Yang L, Li H, Zhang F, Lei L, Li S, Feng X, Li A, He J
[PMID: 26400161]Nucleic Acids Res. 44 (2016) 187-97
Polyketide Ring Expansion Mediated by a Thioesterase, Chain Elongation and Cyclization Domain, in Azinomycin Biosynthesis: Characterization of AziB and AziG.
Mori S, Simkhada D, Zhang H, Erb MS, Zhang Y, Williams H, Fedoseyenko D, Russell WK, Kim D, Fleer N, Ealick SE, Watanabe CM
[PMID: 26731610]Biochemistry. 55 (2016) 704-14

close this sectionData download

Compound nameGenomic
nucleotide
sequence
Nucleotide
sequences of the
coding regions
Translation of the
coding sequences
List of genes/ORFsGBK
Azinomycin B
Genomic nucleotide sequence
64 KB
Nucleotide sequences of the coding regions
60 KB
Translation of the coding sequences
21 KB
List of genes/ORFs
6 KB

 

 
GenBank format
131 KB

close this sectionHistory

  • 2016-12-27[Update]
  • 2016-01-27[Update]
  • 2015-02-12[Update]
  • 2014-06-25[Update]
  • 2014-03-10[Release]