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Cluster information : Nystatin


close this sectionCompound

Entry nameNystatin
PKS TypeTypeI modular
Starter Unitacetyl-CoA
Chain Length19
Sugar Unitmycosamine
ClassificationPolyene Macrolide
ActivityAntifungal
CompositionA1: C47H75NO17
Nystatin

close this sectionOriginal source

Organism
StrainATCC 11455 (=NBRC 15452)
Contig
Click on the icon to see Genetic map.

close this sectionPKS/NRPS Module

Nysta_00130
nysA
0 acetyl-CoA
Nysta_00140
nysB
1 methylmalonyl-CoA
2 methylmalonyl-CoA
not conserved YASHS(S->G)
Nysta_00150
nysC
3 malonyl-CoA
4 malonyl-CoA
5 malonyl-CoA
6 malonyl-CoA
7 malonyl-CoA
8 malonyl-CoA
Nysta_00050
nysI
9 malonyl-CoA
10 malonyl-CoA
11 methylmalonyl-CoA
12 malonyl-CoA
13 malonyl-CoA
14 malonyl-CoA
Nysta_00060
nysJ
15 malonyl-CoA
16 malonyl-CoA
17 malonyl-CoA
Nysta_00070
nysK
18 malonyl-CoA
KS8..380
AT527..838
dh882..1049
ACP1213..1283
KS41..412
AT575..895
dh
KR1201..1381
ACP1485..1555
KS1579..1955
AT2114..2436
dh
KR2749..2929
ACP3037..3107
KS35..405
AT545..855
DH903..1067
KR1381..1561
ACP1662..1732
KS1757..2131
AT2289..2600
DH2649..2812
KR3124..3304
ACP3407..3477
KS3501..3875
AT4031..4343
DH4391..4555
ER4934..5238
KR5248..5428
ACP5524..5598
KS5623..5996
AT6165..6474
DH6523..6698
KR7038..7214
ACP7315..7385
KS7408..7782
AT7938..8251
DH8299..8464
KR8812..8992
ACP9116..9190
KS9214..9588
AT9757..10067
DH10118..10283
KR10657..10837
ACP10935..11009
KS34..398
AT571..886
DH936..1117
KR1443..1619
ACP1720..1790
KS1813..2187
AT2345..2662
dh
KR2983..3162
ACP3266..3336
KS3357..3730
AT3895..4215
dh
KR4523..4703
ACP4806..4876
KS4901..5276
AT5430..5752
dh
KR6068..6247
ACP6348..6418
KS6454..6823
AT6971..7289
dh
kr7535..7710
ACP7813..7883
KS7908..8271
AT8429..8738
dh
KR9050..9229
ACP9331..9401
KS40..415
AT577..886
DH934..1096
ER1427..1730
KR1740..1920
ACP2023..2093
KS2117..2488
AT2633..2950
dh
KR3257..3436
ACP3539..3609
KS3634..4007
AT4151..4469
dh4518..4676
KR4997..5177
ACP5277..5347
KS34..407
AT567..878
dh929..1096
KR1416..1596
ACP1696..1766
TE1854..2058

close this sectionReference

Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway.
Brautaset T, Sekurova ON, Sletta H, Ellingsen TE, StrLm AR, Valla S, Zotchev SB
[PMID: 10873841]Chem Biol. 7 (2000) 395-403
In vivo analysis of the regulatory genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455 reveals their differential control over antibiotic biosynthesis.
Sekurova ON, Brautaset T, Sletta H, Borgos SE, Jakobsen M OM, Ellingsen TE, Strom AR, Valla S, Zotchev SB
[PMID: 14973031]J Bacteriol. 186 (2004) 1345-54
An unexpected role for the putative 4'-phosphopantetheinyl transferase-encoding gene nysF in the regulation of nystatin biosynthesis in Streptomyces noursei ATCC 11455.
Volokhan O, Sletta H, Sekurova ON, Ellingsen TE, Zotchev SB
[PMID: 15990252]FEMS Microbiol Lett. 249 (2005) 57-64
Nystatin biosynthesis and transport: nysH and nysG genes encoding a putative ABC transporter system in Streptomyces noursei ATCC 11455 are required for efficient conversion of 10-deoxynystatin to nystatin.
Sletta H, Borgos SE, Bruheim P, Sekurova ON, Grasdalen H, Aune R, Ellingsen TE, Zotchev SB
[PMID: 16251298]Antimicrob Agents Chemother. 49 (2005) 4576-83
Characterization of the P450 monooxygenase NysL, responsible for C-10 hydroxylation during biosynthesis of the polyene macrolide antibiotic nystatin in Streptomyces noursei.
Volokhan O, Sletta H, Ellingsen TE, Zotchev SB
[PMID: 16597951]Appl Environ Microbiol. 72 (2006) 2514-9
Analysis of the mycosamine biosynthesis and attachment genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455.
Nedal A, Sletta H, Brautaset T, Borgos SE, Sekurova ON, Ellingsen TE, Zotchev SB
[PMID: 17905880]Appl Environ Microbiol. 73 (2007) 7400-7
The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI.
Zhang C, Moretti R, Jiang J, Thorson JS
[PMID: 18798210]Chembiochem. 9 (2008) 2506-14
New nystatin-related antifungal polyene macrolides with altered polyol region generated via biosynthetic engineering of Streptomyces noursei.
Brautaset T, Sletta H, Degnes KF, Sekurova ON, Bakke I, Volokhan O, Andreassen T, Ellingsen TE, Zotchev SB
[PMID: 21764946]Appl Environ Microbiol. 77 (2011) 6636-43
Initiation of polyene macrolide biosynthesis: interplay between polyketide synthase domains and modules as revealed via domain swapping, mutagenesis, and heterologous complementation.
Heia S, Borgos SE, Sletta H, Escudero L, Seco EM, Malpartida F, Ellingsen TE, Zotchev SB
[PMID: 21821762]Appl Environ Microbiol. 77 (2011) 6982-90
In vivo investigation of the substrate recognition capability and activity affecting amino acid residues of glycosyltransferase FscMI in the biosynthesis of candicidin.
Lei X, Kong L, Zhang C, Liu Q, Yao F, Zhang W, Deng Z, You D
[PMID: 23324745]Mol Biosyst. 9 (2013) 422-30
Stereochemistry of reductions catalyzed by methyl-epimerizing ketoreductase domains of polyketide synthases.
You YO, Khosla C, Cane DE
[PMID: 23659177]J Am Chem Soc. 135 (2013) 7406-9
Coupled methyl group epimerization and reduction by polyketide synthase ketoreductase domains. Ketoreductase-catalyzed equilibrium isotope exchange.
Garg A, Khosla C, Cane DE
[PMID: 24161343]J Am Chem Soc. 135 (2013) 16324-7

close this sectionData download

Compound nameGenomic
nucleotide
sequence
Nucleotide
sequences of the
coding regions
Translation of the
coding sequences
List of genes/ORFsGBK
Nystatin
Genomic nucleotide sequence
125 KB
Nucleotide sequences of the coding regions
122 KB
Translation of the coding sequences
42 KB
List of genes/ORFs
3 KB

 

 
GenBank format
228 KB

close this sectionHistory

  • 2014-06-25[Update]
  • 2013-12-27[Update]
  • 2013-09-04[Update]
  • 2013-01-16[Update]
  • 2012-10-02[Update]
  • 2012-03-28[Release]