Cluster information : Nystatin --- DoBISCUIT

Entry name	Nystatin
PKS Type	TypeI modular
Classification	Polyene Macrolide
Starter Unit	acetyl-CoA
Chain Length	19
Sugar Unit	mycosamine
Activity	Antifungal
Composition	A1: C₄₇H₇₅NO₁₇

Organism	Streptomyces noursei
Strain	ATCC 11455 (=NBRC 14542)
Contig	AF263912

Nysta_00130 nysA	0	acetyl-CoA
Nysta_00140 nysB	1	methylmalonyl-CoA
Nysta_00140 nysB	2	methylmalonyl-CoA not conserved YASHS(S->G)
Nysta_00150 nysC	3	malonyl-CoA
	4	malonyl-CoA
	5	malonyl-CoA
	6	malonyl-CoA
	7	malonyl-CoA
	8	malonyl-CoA
Nysta_00050 nysI	9	malonyl-CoA
	10	malonyl-CoA
	11	methylmalonyl-CoA
	12	malonyl-CoA
	13	malonyl-CoA
	14	malonyl-CoA
Nysta_00060 nysJ	15	malonyl-CoA
	16	malonyl-CoA
	17	malonyl-CoA
Nysta_00070 nysK	18	malonyl-CoA

KS	8..380

AT	527..838

dh	882..1049

ACP	1213..1283

KS	41..412

AT	575..895

dh

KR	1201..1381

ACP	1485..1555

KS	1579..1955

AT	2114..2436

dh

KR	2749..2929

ACP	3037..3107

KS	35..405

AT	545..855

DH	903..1067

KR	1381..1561

ACP	1662..1732

KS	1757..2131

AT	2289..2600

DH	2649..2812

KR	3124..3304

ACP	3407..3477

KS	3501..3875

AT	4031..4343

DH	4391..4555

ER	4934..5238

KR	5248..5428

ACP	5524..5598

KS	5623..5996

AT	6165..6474

DH	6523..6698

KR	7038..7214

ACP	7315..7385

KS	7408..7782

AT	7938..8251

DH	8299..8464

KR	8812..8992

ACP	9116..9190

KS	9214..9588

AT	9757..10067

DH	10118..10283

KR	10657..10837

ACP	10935..11009

KS	34..398

AT	571..886

Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway.: Brautaset T, Sekurova ON, Sletta H, Ellingsen TE, StrLm AR, Valla S, Zotchev SB
[PMID: 10873841]Chem Biol. 7 (2000) 395-403
In vivo analysis of the regulatory genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455 reveals their differential control over antibiotic biosynthesis.: Sekurova ON, Brautaset T, Sletta H, Borgos SE, Jakobsen M OM, Ellingsen TE, Strom AR, Valla S, Zotchev SB
[PMID: 14973031]J Bacteriol. 186 (2004) 1345-54
An unexpected role for the putative 4'-phosphopantetheinyl transferase-encoding gene nysF in the regulation of nystatin biosynthesis in Streptomyces noursei ATCC 11455.: Volokhan O, Sletta H, Sekurova ON, Ellingsen TE, Zotchev SB
[PMID: 15990252]FEMS Microbiol Lett. 249 (2005) 57-64
Nystatin biosynthesis and transport: nysH and nysG genes encoding a putative ABC transporter system in Streptomyces noursei ATCC 11455 are required for efficient conversion of 10-deoxynystatin to nystatin.: Sletta H, Borgos SE, Bruheim P, Sekurova ON, Grasdalen H, Aune R, Ellingsen TE, Zotchev SB
[PMID: 16251298]Antimicrob Agents Chemother. 49 (2005) 4576-83
Characterization of the P450 monooxygenase NysL, responsible for C-10 hydroxylation during biosynthesis of the polyene macrolide antibiotic nystatin in Streptomyces noursei.: Volokhan O, Sletta H, Ellingsen TE, Zotchev SB
[PMID: 16597951]Appl Environ Microbiol. 72 (2006) 2514-9
Analysis of the mycosamine biosynthesis and attachment genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455.: Nedal A, Sletta H, Brautaset T, Borgos SE, Sekurova ON, Ellingsen TE, Zotchev SB
[PMID: 17905880]Appl Environ Microbiol. 73 (2007) 7400-7
The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI.: Zhang C, Moretti R, Jiang J, Thorson JS
[PMID: 18798210]Chembiochem. 9 (2008) 2506-14
New nystatin-related antifungal polyene macrolides with altered polyol region generated via biosynthetic engineering of Streptomyces noursei.: Brautaset T, Sletta H, Degnes KF, Sekurova ON, Bakke I, Volokhan O, Andreassen T, Ellingsen TE, Zotchev SB
[PMID: 21764946]Appl Environ Microbiol. 77 (2011) 6636-43
Initiation of polyene macrolide biosynthesis: interplay between polyketide synthase domains and modules as revealed via domain swapping, mutagenesis, and heterologous complementation.: Heia S, Borgos SE, Sletta H, Escudero L, Seco EM, Malpartida F, Ellingsen TE, Zotchev SB
[PMID: 21821762]Appl Environ Microbiol. 77 (2011) 6982-90