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Cluster information : Aclacinomycin A


close this sectionCompound

Entry nameAclacinomycin A
SynonymAclarubicin
PKS TypeTypeII
Starter Unitpropionyl-CoA
Chain Length10
Sugar UnitL-2-deoxyfucose
L-rhodosamine
L-cinerulose A
ClassificationAnthracycline
ActivityAntitumor
Toxic
CompositionC42H53NO15
Aclacinomycin A

close this sectionOriginal source

Organism
StrainATCC 31615
Contig
Click on the icon to see Genetic map.

close this sectionPKS/NRPS Module

KS122..352
KS4..375
CLF6..362
ACP5..77

close this sectionReference

Elucidation of anthracyclinone biosynthesis by stepwise cloning of genes for anthracyclines from three different Streptomyces spp.
Kantola J, Kunnari T, Hautala A, Hakala J, Ylihonko K, Mantsala P
[PMID: 10658662]Microbiology. 146 ( Pt 1) (2000) 155-63
A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin.
Raty K, Kunnari T, Hakala J, Mantsala P, Ylihonko K
[PMID: 11016846]Mol Gen Genet. 264 (2000) 164-72
Cloning and characterization of Streptomyces galilaeus aclacinomycins polyketide synthase (PKS) cluster.
Raty K, Kantola J, Hautala A, Hakala J, Ylihonko K, Mantsala P
[PMID: 12137949]Gene. 293 (2002) 115-22
Characterization of mutations in aclacinomycin A-non-producing Streptomyces galilaeus strains with altered glycosylation patterns.
Raty K, Hautala A, Torkkell S, Kantola J, Mantsala P, Hakala J, Ylihonko K
[PMID: 12427929]Microbiology. 148 (2002) 3375-84
Studies on a second and third ring cyclization in anthracycline biosynthesis.
Hautala A, Torkkell S, Raty K, Kunnari T, Kantola J, Mantsala P, Hakala J, Ylihonko K
[PMID: 12715874]J Antibiot (Tokyo). 56 (2003) 143-53
AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A.
Lu W, Leimkuhler C, Oberthur M, Kahne D, Walsh CT
[PMID: 15078101]Biochemistry. 43 (2004) 4548-58
AknT is an activating protein for the glycosyltransferase AknS in L-aminodeoxysugar transfer to the aglycone of aclacinomycin A.
Lu W, Leimkuhler C, Gatto GJ Jr, Kruger RG, Oberthur M, Kahne D, Walsh CT
[PMID: 15911373]Chem Biol. 12 (2005) 527-34
Crystal structure of the polyketide cyclase AknH with bound substrate and product analogue: implications for catalytic mechanism and product stereoselectivity.
Kallio P, Sultana A, Niemi J, Mantsala P, Schneider G
[PMID: 16414075]J Mol Biol. 357 (2006) 210-20
Structure determination by multiwavelength anomalous diffraction of aclacinomycin oxidoreductase: indications of multidomain pseudomerohedral twinning.
Sultana A, Alexeev I, Kursula I, Mantsala P, Niemi J, Schneider G
[PMID: 17242508]Acta Crystallogr D Biol Crystallogr. 63 (2007) 149-59
Aclacinomycin oxidoreductase (AknOx) from the biosynthetic pathway of the antibiotic aclacinomycin is an unusual flavoenzyme with a dual active site.
Alexeev I, Sultana A, Mantsala P, Niemi J, Schneider G
[PMID: 17395717]Proc Natl Acad Sci U S A. 104 (2007) 6170-5
Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A.
Leimkuhler C, Fridman M, Lupoli T, Walker S, Walsh CT, Kahne D
[PMID: 17685523]J Am Chem Soc. 129 (2007) 10546-50

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Compound nameGenomic
nucleotide
sequence
Nucleotide
sequences of the
coding regions
Translation of the
coding sequences
List of genes/ORFsGBK
Aclacinomycin A
Genomic nucleotide sequence
30 KB
Nucleotide sequences of the coding regions
9 KB
Translation of the coding sequences
6 KB
List of genes/ORFs
2 KB

 

 
GenBank format
66 KB
AF257324
Genomic nucleotide sequence
15 KB
Nucleotide sequences of the coding regions
15 KB
Translation of the coding sequences
6 KB
List of genes/ORFs
2 KB

 

 

 
AF264025
Genomic nucleotide sequence
15 KB
Nucleotide sequences of the coding regions
14 KB
Translation of the coding sequences
5 KB
List of genes/ORFs
2 KB

 

 

 

close this sectionHistory

  • 2013-01-16[Update]
  • 2012-10-02[Release]