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Cluster information : A47934


close this sectionCompound

Entry nameA47934
Moiety descriptionA: aglycone
B: L-3,5-dihydroxyphenylglycine(Dpg)
PKS TypeA: NRPS
B: TypeIII
Starter UnitB: malonyl-CoA
Chain LengthB: 4
Sugar Unitnone
ClassificationGlycopeptide(teicoplanin-type)
ActivityAntibacterial
CompositionC58H44Cl3N7O21S
A47934

close this sectionOriginal source

Organism
StrainNRRL 15009
Contig
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close this sectionPKS/NRPS Module

AA4793_00130
staA
1 L-4-hydroxyphenylglycine(Hpg)
2 tyrosine
A4793_00120
staB
3 L-3,5-dihydroxyphenylglycine(Dpg)
A4793_00110
staC
4 L-4-hydroxyphenylglycine(Hpg)
5 L-4-hydroxyphenylglycine(Hpg)
6 tyrosine
A4793_00100
staD
7 L-3,5-dihydroxyphenylglycine(Dpg)
BA4793_00220
dpgA
1
A35..425
PCP499..565
C599..890
A1057..1447
PCP1521..1587
E1627..2057
C15..310
A489..879
PCP955..1021
e1057..1483
C7..301
A481..878
PCP956..1022
E1056..1496
C1505..1796
A1977..2369
PCP2452..2518
E2560..3004
C3013..3307
A3489..3877
PCP3963..4029
C15..310
A493..889
PCP971..1038
X1053..1352
TE1626..1839
CHS102..388

close this sectionReference

D-Ala-D-Ala ligases from glycopeptide antibiotic-producing organisms are highly homologous to the enterococcal vancomycin-resistance ligases VanA and VanB.
Marshall CG, Broadhead G, Leskiw BK, Wright GD
[PMID: 9177243]Proc Natl Acad Sci U S A. 94 (1997) 6480-3
Homologs of the vancomycin resistance D-Ala-D-Ala dipeptidase VanX in Streptomyces toyocaensis, Escherichia coli and Synechocystis: attributes of catalytic efficiency, stereoselectivity and regulation with implications for function.
Lessard IA, Pratt SD, McCafferty DG, Bussiere DE, Hutchins C, Wanner BL, Katz L, Walsh CT
[PMID: 9751644]Chem Biol. 5 (1998) 489-504
Molecular mechanism of VanHst, an alpha-ketoacid dehydrogenase required for glycopeptide antibiotic resistance from a glycopeptide producing organism.
Marshall CG, Zolli M, Wright GD
[PMID: 10387095]Biochemistry. 38 (1999) 8485-91
Assembling the glycopeptide antibiotic scaffold: The biosynthesis of A47934 from Streptomyces toyocaensis NRRL15009.
Pootoolal J, Thomas MG, Marshall CG, Neu JM, Hubbard BK, Walsh CT, Wright GD
[PMID: 12060705]Proc Natl Acad Sci U S A. 99 (2002) 8962-7
Comparative analysis and insights into the evolution of gene clusters for glycopeptide antibiotic biosynthesis.
Donadio S, Sosio M, Stegmann E, Weber T, Wohlleben W
[PMID: 16007453]Mol Genet Genomics. 274 (2005) 40-50
Biosynthesis of sulfated glycopeptide antibiotics by using the sulfotransferase StaL.
Lamb SS, Patel T, Koteva KP, Wright GD
[PMID: 16492565]Chem Biol. 13 (2006) 171-81
Crystal structure of StaL, a glycopeptide antibiotic sulfotransferase from Streptomyces toyocaensis.
Shi R, Lamb SS, Bhat S, Sulea T, Wright GD, Matte A, Cygler M
[PMID: 17329243]J Biol Chem. 282 (2007) 13073-86
Structural basis for cofactor-independent dioxygenation in vancomycin biosynthesis.
Widboom PF, Fielding EN, Liu Y, Bruner SD
[PMID: 17507985]Nature. 447 (2007) 342-5
The biosynthesis of teicoplanin-type glycopeptide antibiotics: assignment of p450 mono-oxygenases to side chain cyclizations of glycopeptide a47934.
Hadatsch B, Butz D, Schmiederer T, Steudle J, Wohlleben W, Sussmuth R, Stegmann E
[PMID: 17884639]Chem Biol. 14 (2007) 1078-89
Substrate recognition and catalysis by the cofactor-independent dioxygenase DpgC.
Fielding EN, Widboom PF, Bruner SD
[PMID: 18004875]Biochemistry. 46 (2007) 13994-4000
Not so clear on oxygen. Comment on Structural basis for cofactor-independent dioxygenation in vancomycin biosynthesis by Widboom et al. (2007), Nature (London), 447, 342-345.
Stec B, Stieglitz KA
[PMID: 18703850]Acta Crystallogr D Biol Crystallogr. 64 (2008) 1000-2
A novel membrane protein, VanJ, conferring resistance to teicoplanin.
Novotna G, Hill C, Vincent K, Liu C, Hong HJ
[PMID: 22232274]Antimicrob Agents Chemother. 56 (2012) 1784-96
Sulfonation of glycopeptide antibiotics by sulfotransferase StaL depends on conformational flexibility of aglycone scaffold.
Shi R, Munger C, Kalan L, Sulea T, Wright GD, Cygler M
[PMID: 22753479]Proc Natl Acad Sci U S A. 109 (2012) 11824-9
Glycopeptide antibiotic biosynthesis.
Yim G, Thaker MN, Koteva K, Wright G
[PMID: 24220108]J Antibiot (Tokyo). 67 (2014) 31-41
Genome Sequence of Streptomyces toyocaensis NRRL 15009, Producer of the Glycopeptide Antibiotic A47934.
Kwun MJ, Hong HJ
[PMID: 25081262]Genome Announc. 2 (2014)
Understanding the crucial interactions between Cytochrome P450s and non-ribosomal peptide synthetases during glycopeptide antibiotic biosynthesis.
Peschke M, Gonsior M, Sussmuth RD, Cryle MJ
[PMID: 27289043]Curr Opin Struct Biol. 41 (2016) 46-53

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Compound nameGenomic
nucleotide
sequence
Nucleotide
sequences of the
coding regions
Translation of the
coding sequences
List of genes/ORFsGBK
A47934
Genomic nucleotide sequence
70 KB
Nucleotide sequences of the coding regions
67 KB
Translation of the coding sequences
23 KB
List of genes/ORFs
5 KB

 

 
GenBank format
138 KB

close this sectionHistory

  • 2016-12-27[Release]