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Cluster information : Lasalocid


close this sectionCompound

Entry nameLasalocid
PKS TypeTypeI modular
Starter Unitmalonyl-CoA
Chain Length12
Sugar Unitnone
ClassificationPolyether
ActivityAntibacterial
Ionophore
CompositionA: C34H54O8
Lasalocid

close this sectionOriginal source

Organism
StrainATCC 35851
Contig
Click on the icon to see Genetic map.

close this sectionPKS/NRPS Module

Lasal_00130
lsd11
0 malonyl-CoA
1 ethylmalonyl-CoA
2 malonyl-CoA
Lasal_00140
lsd12
3 ethylmalonyl-CoA
4 methylmalonyl-CoA
5 ethylmalonyl-CoA
Lasal_00150
lsd13
6 methylmalonyl-CoA
Lasal_00160
lsd14
7 methylmalonyl-CoA
Lasal_00170
lsd15
8 malonyl-CoA
9 malonyl-CoA
Lasal_00180
lsd16
10 methylmalonyl-CoA
Lasal_00190
lsd17
11 malonyl-CoA
KSQ17..381
AT536..858
ACP959..1029
KS1052..1428
AT1581..1892
DH1940..2108
KR2476..2656
ACP2762..2832
KS2857..3233
AT3385..3693
DH3739..3900
ER4240..4533
KR4543..4723
ACP4832..4902
KS34..410
AT565..876
DH920..1085
KR1412..1592
ACP1693..1767
KS1789..2164
AT2322..2640
DH2689..2863
ER3179..3471
KR3481..3661
ACP3766..3836
KS3860..4236
AT4388..4699
KR5020..5199
ACP5304..5374
KS40..416
AT568..887
ACP959..1029
KS38..414
AT566..885
KR1201..1381
ACP1488..1563
KS35..411
AT564..872
DH917..1086
ER1443..1736
KR1746..1926
ACP2032..2102
KS2126..2502
AT2654..2957
ACP3519..3589
KS58..434
AT604..921
dh971..1135
KR1462..1642
ACP1747..1817
KS35..411
AT566..868
ACP937..1007
TE1105..1307

close this sectionReference

Epoxide hydrolase Lsd19 for polyether formation in the biosynthesis of lasalocid A: direct experimental evidence on polyene-polyepoxide hypothesis in polyether biosynthesis.
Shichijo Y, Migita A, Oguri H, Watanabe M, Tokiwano T, Watanabe K, Oikawa H
[PMID: 18710235]J Am Chem Soc. 130 (2008) 12230-1
Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis.
Migita A, Watanabe M, Hirose Y, Watanabe K, Tokiwano T, Kinashi H, Oikawa H
[PMID: 19129623]Biosci Biotechnol Biochem. 73 (2009) 169-76
Intriguing substrate tolerance of epoxide hydrolase Lsd19 involved in biosynthesis of the ionophore antibiotic lasalocid A.
Matsuura Y, Shichijo Y, Minami A, Migita A, Oguri H, Watanabe M, Tokiwano T, Watanabe K, Oikawa H
[PMID: 20394359]Org Lett. 12 (2010) 2226-9
Enzymatic epoxide-opening cascades catalyzed by a pair of epoxide hydrolases in the ionophore polyether biosynthesis.
Minami A, Migita A, Inada D, Hotta K, Watanabe K, Oguri H, Oikawa H
[PMID: 21375229]Org Lett. 13 (2011) 1638-41
Enzymatic catalysis of anti-Baldwin ring closure in polyether biosynthesis.
Hotta K, Chen X, Paton RS, Minami A, Li H, Swaminathan K, Mathews II, Watanabe K, Oikawa H, Houk KN, Kim CY
[PMID: 22388816]Nature. 483 (2012) 355-8
Sequential enzymatic epoxidation involved in polyether lasalocid biosynthesis.
Minami A, Shimaya M, Suzuki G, Migita A, Shinde SS, Sato K, Watanabe K, Tamura T, Oguri H, Oikawa H
[PMID: 22506807]J Am Chem Soc. 134 (2012) 7246-9
Biosynthetic machinery of ionophore polyether lasalocid: enzymatic construction of polyether skeleton.
Minami A, Oguri H, Watanabe K, Oikawa H
[PMID: 23796908]Curr Opin Chem Biol. 17 (2013) 555-61
Epoxide hydrolase-lasalocid a structure provides mechanistic insight into polyether natural product biosynthesis.
Wong FT, Hotta K, Chen X, Fang M, Watanabe K, Kim CY
[PMID: 25535803]J Am Chem Soc. 137 (2015) 86-9

close this sectionData download

Compound nameGenomic
nucleotide
sequence
Nucleotide
sequences of the
coding regions
Translation of the
coding sequences
List of genes/ORFsGBK
Lasalocid
Genomic nucleotide sequence
83 KB
Nucleotide sequences of the coding regions
80 KB
Translation of the coding sequences
28 KB
List of genes/ORFs
3 KB

 

 
GenBank format
156 KB

close this sectionHistory

  • 2015-02-12[Update]
  • 2014-06-25[Update]
  • 2013-12-27[Update]
  • 2012-08-03[Update]
  • 2012-06-04[Update]
  • 2012-04-26[Release]