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Cluster information : Phoslactomycin


close this sectionCompound

Entry namePhoslactomycin
PKS TypeTypeI modular
Starter Unitcyclohexylcarbonyl-CoA
Chain Length8
Sugar Unitnone
Classification
ActivityAntifungal
Antitumor
CompositionA: C29H46NO10P
Phoslactomycin

close this sectionOriginal source

Organism
StrainHK803
Contig
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close this sectionPKS/NRPS Module

Phosl_00180
plm1
0
1 malonyl-CoA
Phosl_00190
plm2-3
2 malonyl-CoA
3 malonyl-CoA
Phosl_00060
plm4
4 ethylmalonyl-CoA
not conserved XAGHS
Phosl_00070
plm5
5 malonyl-CoA
Phosl_00080
plm6
6 ethylmalonyl-CoA
not conserved XAGHS
Phosl_00090
plm7
7 malonyl-CoA
AT201..517
ACP567..640
KS661..1034
AT1188..1491
DH1534..1698
KR1998..2177
ACP2283..2353
KS38..411
AT564..867
DH908..1075
KR1383..1563
ACP1662..1732
KS1755..2131
AT2310..2611
KR2931..3110
ACP3207..3277
KS4..352
AT510..829
KR1157..1336
ACP1446..1516
KS34..410
AT565..886
DH931..1095
KR1423..1603
ACP1710..1780
KS13..382
AT546..861
KR1141..1321
ACP1426..1496
KS27..405
AT551..852
KR1149..1330
ACP1432..1502
TE1595..1799

close this sectionReference

Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster.
Palaniappan N, Kim BS, Sekiyama Y, Osada H, Reynolds KA
[PMID: 12819191]J Biol Chem. 278 (2003) 35552-7
The plmS2-encoded cytochrome P450 monooxygenase mediates hydroxylation of phoslactomycin B in Streptomyces sp. strain HK803.
Ghatge MS, Reynolds KA
[PMID: 16291670]J Bacteriol. 187 (2005) 7970-6
Genetic manipulation of the biosynthetic process leading to phoslactomycins, potent protein phosphatase 2A inhibitors.
Ghatge M, Palaniappan N, Das Choudhuri S, Reynolds K
[PMID: 16609856]J Ind Microbiol Biotechnol. 33 (2006) 589-99
Modular polyketide synthases and cis double bond formation: establishment of activated cis-3-cyclohexylpropenoic acid as the diketide intermediate in phoslactomycin biosynthesis.
Alhamadsheh MM, Palaniappan N, Daschouduri S, Reynolds KA
[PMID: 17256943]J Am Chem Soc. 129 (2007) 1910-1
Application of a newly identified and characterized 18-o-acyltransferase in chemoenzymatic synthesis of selected natural and nonnatural bioactive derivatives of phoslactomycins.
Ghatge MS, Palaniappan N, Alhamadsheh MM, DiBari J, Reynolds KA
[PMID: 19304832]Appl Environ Microbiol. 75 (2009) 3469-76
Structural and stereochemical analysis of a modular polyketide synthase ketoreductase domain required for the generation of a cis-alkene.
Bonnett SA, Whicher JR, Papireddy K, Florova G, Smith JL, Reynolds KA
[PMID: 23790488]Chem Biol. 20 (2013) 772-83

close this sectionData download

Compound nameGenomic
nucleotide
sequence
Nucleotide
sequences of the
coding regions
Translation of the
coding sequences
List of genes/ORFsGBK
Phoslactomycin
Genomic nucleotide sequence
77 KB
Nucleotide sequences of the coding regions
72 KB
Translation of the coding sequences
25 KB
List of genes/ORFs
4 KB

 

 
GenBank format
148 KB

close this sectionHistory

  • 2016-01-27[Update]
  • 2013-09-04[Update]
  • 2012-04-26[Release]