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Cluster information : Pimaricin


close this sectionCompound

Entry namePimaricin
SynonymNatamycin
PKS TypeTypeI modular
Starter Unitacetyl-CoA
Chain Length13
Sugar Unitmycosamine
ClassificationPolyene Macrolide
ActivityAntifungal
CompositionC33H47NO13
Pimaricin

close this sectionOriginal source

Organism
StrainATCC 27448 (=NBRC 13367)
Contig

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close this sectionPKS/NRPS Module

Pimar_00160
pimS0
0
Pimar_00170
pimS1
1 malonyl-CoA
2 malonyl-CoA
3 malonyl-CoA
4 malonyl-CoA
Pimar_00070
pimS2
5 malonyl-CoA
6 malonyl-CoA
7 methylmalonyl-CoA
8 malonyl-CoA
9 malonyl-CoA
10 malonyl-CoA
Pimar_00060
pimS3
11 malonyl-CoA
Pimar_00050
pimS4
12 malonyl-CoA
CoL57..462
ACP560..642
ks661..1037
AT1209..1532
ACP1722..1766
KS34..410
AT565..885
KR1191..1375
ACP1470..1540
KS1561..1933
AT2104..2414
DH2463..2629
KR2928..3104
ACP3204..3274
KS3299..3673
AT3829..4140
DH4189..4349
KR4631..4807
ACP4905..4975
KS5002..5374
AT5543..5854
DH5903..6063
KR6357..6533
ACP6636..6706
KS34..398
AT561..887
DH935..1097
KR1416..1596
ACP1697..1767
KS1789..2162
AT2318..2636
KR2960..3139
ACP3235..3309
KS3331..3703
AT3859..4177
KR4487..4666
ACP4767..4837
KS4858..5234
AT5393..5714
KR6025..6204
ACP6309..6379
KS6402..6773
AT6914..7219
kr7523..7701
ACP7804..7874
KS7902..8273
AT8431..8743
KR9061..9240
ACP9343..9413
KS35..408
AT552..857
DH905..1062
KR1371..1551
ACP1652..1722
KS38..410
AT551..856
DH904..1061
KR1370..1550
ACP1651..1721
TE1809..2018

close this sectionReference

The biosynthetic gene cluster for the 26-membered ring polyene macrolide pimaricin. A new polyketide synthase organization encoded by two subclusters separated by functionalization genes.
Aparicio JF, Colina AJ, Ceballos E, Martin JF
[PMID: 10187796]J Biol Chem. 274 (1999) 10133-9
A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis.
Aparicio JF, Fouces R, Mendes MV, Olivera N, Martin JF
[PMID: 11094342]Chem Biol. 7 (2000) 895-905
Identification of PimR as a positive regulator of pimaricin biosynthesis in Streptomyces natalensis.
Anton N, Mendes MV, Martin JF, Aparicio JF
[PMID: 15090496]J Bacteriol. 186 (2004) 2567-75
Characterization of the polyene macrolide P450 epoxidase from Streptomyces natalensis that converts de-epoxypimaricin into pimaricin.
Mendes MV, Anton N, Martin JF, Aparicio JF
[PMID: 15228385]Biochem J. 386 (2005) 57-62
Cholesterol oxidases act as signaling proteins for the biosynthesis of the polyene macrolide pimaricin.
Mendes MV, Recio E, Anton N, Guerra SM, Santos-Aberturas J, Martin JF, Aparicio JF
[PMID: 17379143]Chem Biol. 14 (2007) 279-90
PimM, a PAS domain positive regulator of pimaricin biosynthesis in Streptomyces natalensis.
Anton N, Santos-Aberturas J, Mendes MV, Guerra SM, Martin JF, Aparicio JF
[PMID: 17768260]Microbiology. 153 (2007) 3174-83
Enzymology of the polyenes pimaricin and candicidin biosynthesis.
Martin JF, Aparicio JF
[PMID: 19362642]Methods Enzymol. 459 (2009) 215-42
PimT, an amino acid exporter controls polyene production via secretion of the quorum sensing pimaricin-inducer PI-factor in Streptomyces natalensis.
Vicente CM, Santos-Aberturas J, Guerra SM, Payero TD, Martin JF, Aparicio JF
[PMID: 19505319]Microb Cell Fact. 8 (2009) 33
Molecular control of polyene macrolide biosynthesis: direct binding of the regulator PimM to eight promoters of pimaricin genes and identification of binding boxes.
Santos-Aberturas J, Vicente CM, Guerra SM, Payero TD, Martin JF, Aparicio JF
[PMID: 21187288]J Biol Chem. 286 (2011) 9150-61
Hierarchical Control on Polyene Macrolide Biosynthesis: PimR Modulates Pimaricin Production via the PAS-LuxR Transcriptional Activator PimM.
Santos-Aberturas J, Vicente CM, Payero TD, Martin-Sanchez L, Canibano C, Martin JF, Aparicio JF
[PMID: 22693644]PLoS One. 7 (2012) e38536
In vivo investigation of the substrate recognition capability and activity affecting amino acid residues of glycosyltransferase FscMI in the biosynthesis of candicidin.
Lei X, Kong L, Zhang C, Liu Q, Yao F, Zhang W, Deng Z, You D
[PMID: 23324745]Mol Biosyst. 9 (2013) 422-30
Biotechnological production and application of the antibiotic pimaricin: biosynthesis and its regulation.
Aparicio JF, Barreales EG, Payero TD, Vicente CM, de Pedro A, Santos-Aberturas J
[PMID: 26512010]Appl Microbiol Biotechnol. 100 (2016) 61-78

close this sectionData download

Compound nameGenomic
nucleotide
sequence
Nucleotide
sequences of the
coding regions
Translation of the
coding sequences
List of genes/ORFsGBK
Pimaricin
Genomic nucleotide sequence
93 KB
Nucleotide sequences of the coding regions
25 KB
Translation of the coding sequences
15 KB
List of genes/ORFs
1 KB

 

 
GenBank format
170 KB
FM864219
Genomic nucleotide sequence
3 KB
Nucleotide sequences of the coding regions
717 B
Translation of the coding sequences
272 B
List of genes/ORFs
275 B

 

 

 
AM493721
Genomic nucleotide sequence
4 KB
Nucleotide sequences of the coding regions
3 KB
Translation of the coding sequences
1 KB
List of genes/ORFs
424 B

 

 

 
AJ278573
Genomic nucleotide sequence
86 KB
Nucleotide sequences of the coding regions
83 KB
Translation of the coding sequences
28 KB
List of genes/ORFs
2 KB

 

 

 

close this sectionHistory

  • 2016-12-27[Update]
  • 2013-05-28[Update]
  • 2013-01-16[Update]
  • 2012-10-02[Update]
  • 2012-03-28[Release]