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Cluster information : Chlorothricin


close this sectionCompound

Entry nameChlorothricin
Moiety descriptionA: aglycone
B: 5-chloro-6-methyl-O-methylsalicylic acid moiety
PKS TypeA: TypeI modular
B: TypeI iterative
Starter UnitA: malonyl-CoA
B: acetyl-CoA
Chain LengthA: 12
B: 4
Sugar UnitD-olivose
ClassificationTetronic Acid Derivative
ActivityAntibacterial
CompositionC50H63ClO16
Chlorothricin

close this sectionOriginal source

Organism
StrainDSM 40725
Contig
Click on the icon to see Genetic map.

close this sectionPKS/NRPS Module

AChth_00260
chlA1
0 malonyl-CoA
1 methylmalonyl-CoA
2 malonyl-CoA
Chth_00270
chlA2
3 malonyl-CoA
4 malonyl-CoA
Chth_00290
chlA3
5 malonyl-CoA
6 malonyl-CoA
7 malonyl-CoA
Chth_00300
chlA4
8 malonyl-CoA
Chth_00310
chlA5
9 malonyl-CoA
10 methylmalonyl-CoA
Chth_00320
chlA6
11 malonyl-CoA
BChth_00060
chlB1
1 acetyl-CoA
malonyl-CoA
KSQ44..418
AT588..913
ACP994..1064
KS1087..1462
AT1638..1956
DH2005..2181
KR2506..2686
ACP2802..2872
KS2894..3270
AT3425..3736
DH3785..3949
KR4248..4429
ACP4537..4607
KS45..422
AT573..884
DH932..1099
KR1415..1595
ACP1692..1762
KS1788..2164
AT2322..2636
DH2685..2857
ER3198..3507
KR3517..3697
ACP3802..3872
KS34..409
AT565..876
DH925..1087
ER1427..1732
KR1757..1940
ACP2045..2115
KS2142..2519
AT2674..2985
DH3034..3202
KR3524..3704
ACP3801..3871
KS3899..4276
AT4429..4740
DH4789..4958
KR5275..5455
ACP5557..5627
KS36..413
AT566..880
dh929..1093
KR1416..1596
ACP1695..1765
KS33..409
AT564..876
DH924..1096
ER1435..1740
KR1750..1930
ACP2030..2104
KS2127..2504
AT2658..2976
DH3024..3189
KR3506..3692
ACP3788..3858
KS34..411
AT568..887
kr1225..1402
ACP1495..1564
KS17..391
AT556..871
TH879..1022
KR1380..1575
ACP1661..1734

close this sectionReference

Cloning and characterization of a bacterial iterative type I polyketide synthase gene encoding the 6-methylsalicyclic acid synthase.
Shao L, Qu XD, Jia XY, Zhao QF, Tian ZH, Wang M, Tang GL, Liu W
[PMID: 16677607]Biochem Biophys Res Commun. 345 (2006) 133-9
Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis.
Jia XY, Tian ZH, Shao L, Qu XD, Zhao QF, Tang J, Tang GL, Liu W
[PMID: 16793515]Chem Biol. 13 (2006) 575-85
Cloning and characterization of the tetrocarcin A gene cluster from Micromonospora chalcea NRRL 11289 reveals a highly conserved strategy for tetronate biosynthesis in spirotetronate antibiotics.
Fang J, Zhang Y, Huang L, Jia X, Zhang Q, Zhang X, Tang G, Liu W
[PMID: 18586939]J Bacteriol. 190 (2008) 6014-25
Dissection of two acyl-transfer reactions centered on acyl-S-carrier protein intermediates for incorporating 5-chloro-6-methyl-O-methylsalicyclic acid into chlorothricin.
He QL, Jia XY, Tang MC, Tian ZH, Tang GL, Liu W
[PMID: 19266533]Chembiochem. 10 (2009) 813-9
Insights into bacterial 6-methylsalicylic acid synthase and its engineering to orsellinic acid synthase for spirotetronate generation.
Ding W, Lei C, He Q, Zhang Q, Bi Y, Liu W
[PMID: 20534347]Chem Biol. 17 (2010) 495-503
Coordinative Modulation of Chlorothricin Biosynthesis by Binding of the Glycosylated Intermediates and End Product to a Responsive Regulator ChlF1.
Li Y, Li J, Tian Z, Xu Y, Zhang J, Liu W, Tan H
[PMID: 26750095]J Biol Chem. 291 (2016) 5406-17
Insights into 6-Methylsalicylic Acid Bio-assembly by Using Chemical Probes.
Parascandolo JS, Havemann J, Potter HK, Huang F, Riva E, Connolly J, Wilkening I, Song L, Leadlay PF, Tosin M
[PMID: 26833898]Angew Chem Int Ed Engl. 55 (2016) 3463-7

close this sectionData download

Compound nameGenomic
nucleotide
sequence
Nucleotide
sequences of the
coding regions
Translation of the
coding sequences
List of genes/ORFsGBK
Chlorothricin
Genomic nucleotide sequence
113 KB
Nucleotide sequences of the coding regions
108 KB
Translation of the coding sequences
37 KB
List of genes/ORFs
6 KB

 

 
GenBank format
214 KB

close this sectionHistory

  • 2016-12-27[Update]
  • 2012-10-02[Update]
  • 2012-08-03[Update]
  • 2012-04-26[Release]