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Cluster information : Kirromycin


close this sectionCompound

Entry nameKirromycin
PKS TypeTrans-AT
PKS-NRPS hybrid
Starter Unitacetyl-CoA
Chain Length15
Sugar Unitnone
ClassificationPeptide-Polyketide Hybrid
ActivityAntibacterial
CompositionC43H60N2O12
Kirromycin

close this sectionOriginal source

Organism
StrainTü365
Contig
Click on the icon to see Genetic map.

close this sectionPKS/NRPS Module

Kirro_00220
kirAI
0
1
2
Kirro_00230
kirAII
2
3
4
5
Kirro_00240
kirAIII
6 glycine
7
Kirro_00250
kirAIV
7
8
9
10
11
12
Kirro_00260
kirAV
12
13
Kirro_00270
kirAVI
14 methylmalonyl-CoA
15 malonyl-CoA
not conserved HAFHS(H->T)
Kirro_00290
kirB
16 beta-alanine
Kirro_00300
kirCI
17 malonyl-CoA
Kirro_00340
kirCII
18 ethylmalonyl-CoA
ACP292..365
KS386..757
KR1113..1313
ACP1393..1462
KS1516..1885
DH16..155
ACP301..371
KR582..743
KS850..1215
KR1697..1864
MT2121..2224
ACP2329..2398
KS2433..2802
KR3171..3347
ACP3435..3504
KS3557..3936
ACP4212..4281
C37..336
A521..900
PCP998..1067
KS1126..1491
DH139..292
KR565..738
ACP821..889
KS937..1304
DH1512..1668
KR2002..2183
MT2433..2536
ACP2648..2716
KS2778..3139
ACP3429..3498
KS3516..3891
KR4295..4477
ACP4562..4631
KS4698..5066
KR5492..5693
ACP5829..5898
KS5928..6296
DH2..155
ACP286..357
KR555..727
KS808..1177
DH1447..1592
KR1878..2044
ACP2123..2197
KS34..394
AT549..865
DH908..1065
KR1343..1516
ACP1615..1685
KS1706..2078
AT2227..2537
ACP2821..2891
C18..286
A483..875
PCP963..1030
at5..305
AT347..601
er660..944
AT91..400

close this sectionReference

Molecular analysis of the kirromycin biosynthetic gene cluster revealed beta-alanine as precursor of the pyridone moiety.
Weber T, Laiple KJ, Pross EK, Textor A, Grond S, Welzel K, Pelzer S, Vente A, Wohlleben W
[PMID: 18291322]Chem Biol. 15 (2008) 175-88
The kirromycin gene cluster of Streptomyces collinus Tu 365 codes for an aspartate-alpha-decarboxylase, KirD, which is involved in the biosynthesis of the precursor beta-alanine.
Laiple KJ, Hartner T, Fiedler HP, Wohlleben W, Weber T
[PMID: 19609288]J Antibiot (Tokyo). 62 (2009) 465-8
The phosphopantetheinyl transferase KirP activates the ACP and PCP domains of the kirromycin NRPS/PKS of Streptomyces collinus Tu 365.
Pavlidou M, Pross EK, Musiol EM, Kulik A, Wohlleben W, Weber T
[PMID: 21401713]FEMS Microbiol Lett. 319 (2011) 26-33
Supramolecular templating in kirromycin biosynthesis: the acyltransferase KirCII loads ethylmalonyl-CoA extender onto a specific ACP of the trans-AT PKS.
Musiol EM, Hartner T, Kulik A, Moldenhauer J, Piel J, Wohlleben W, Weber T
[PMID: 21513880]Chem Biol. 18 (2011) 438-44
Poly specific trans-acyltransferase machinery revealed via engineered acyl-CoA synthetases.
Koryakina I, McArthur J, Randall S, Draelos MM, Musiol EM, Muddiman DC, Weber T, Williams GJ
[PMID: 23083014]ACS Chem Biol. 8 (2013) 200-8
The AT2 Domain of KirCI Loads Malonyl Extender Units to the ACPs of the Kirromycin PKS.
Musiol EM, Greule A, Hartner T, Kulik A, Wohlleben W, Weber T
[PMID: 23828654]Chembiochem. 14 (2013) 1343-52
Complete genome sequence of the kirromycin producer Streptomyces collinus Tu 365 consisting of a linear chromosome and two linear plasmids.
Ruckert C, Szczepanowski R, Albersmeier A, Goesmann A, Iftime D, Musiol EM, Blin K, Wohlleben W, Puhler A, Kalinowski J, Weber T
[PMID: 24140291]J Biotechnol. 168 (2013) 739-40
Reprogramming Acyl Carrier Protein Interactions of an Acyl-CoA Promiscuous trans-Acyltransferase.
Ye Z, Musiol EM, Weber T, Williams GJ
[PMID: 24726832]Chem Biol. 21 (2014) 636-46
Comparative analysis of the substrate specificity of trans- versus cis-acyltransferases of assembly line polyketide synthases.
Dunn BJ, Watts KR, Robbins T, Cane DE, Khosla C
[PMID: 24871074]Biochemistry. 53 (2014) 3796-806
Discovery of a new family of Dieckmann cyclases essential to tetramic acid and pyridone-based natural products biosynthesis.
Gui C, Li Q, Mo X, Qin X, Ma J, Ju J
[PMID: 25621700]Org Lett. 17 (2015) 628-31

close this sectionData download

Compound nameGenomic
nucleotide
sequence
Nucleotide
sequences of the
coding regions
Translation of the
coding sequences
List of genes/ORFsGBK
Kirromycin
Genomic nucleotide sequence
134 KB
Nucleotide sequences of the coding regions
128 KB
Translation of the coding sequences
45 KB
List of genes/ORFs
7 KB

 

 
GenBank format
249 KB

close this sectionHistory

  • 2016-01-27[Update]
  • 2014-06-25[Update]
  • 2013-12-27[Update]
  • 2012-10-02[Update]
  • 2012-08-03[Release]